Procedures of Mitochondria Purification and Gene Expression to Study Alternative Respiratory and Uncoupling Pathways in Fruits

Oliveira, Jurandi Gonçalves de; Morales, Luis Miguel Mazorra; Silva, Gláucia Michelle Cosme; Saraiva, Kátia Daniella da Cruz; Santana, Diederson Bortolino; Santos, Clesivan Pereira dos; Oliveira, Marcos Góes; Costa, José Hélio

doi:10.1007/978-1-4939-7292-0_13

Cited by 2 publications

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References 19 publications

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“…Additionally, according with [2] one of the most widely used techniques that has guaranteed effects on large scale conservation is refrigeration. This effect is mainly due to the alteration in metabolic rates, affecting the respiratory process, which in turn delays ripening in climacteric fruits, which leads to an increase in the shelf life of these fruits [3]. However, bananas fruit when stored at temperatures below 13 °C, there is a classical disorders associated with chilling injury, called chilling, causing the skin browning, affecting the visually quality-trait which leads to lower acceptance by consumers [4].…”

Section: Introductionmentioning

confidence: 99%

The Hydrothermal Treatment Associated with Calcium Chloride Improve Banana cv. ‘Prata Gorutuba’ Quality Modulating Primary Metabolism

Paraizo

Jesus

Aguiar

et al. 2019

AFSJ

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The temperature is one of the most used techniques to maintain fruit quality over long time storage and consequently the fruit respiratory metabolism is directly influenced. The combined use of CaCl2 and hydrothermal treatment can be an important alternative to improve banana fruit quality and influences the ratio starch/sugar and consequently quality traits. Based on the hypothesis the calcium and hydrothermal works synergistically modulating banana primary metabolism and skin color changes. The starch content and chrome parameters were kept in higher values at 2% e 3% (w/v) of CaCl2. However, the fruit storage at control condition have shown lower fresh weight loss (%), followed by total soluble solids and sugars content. In addition, our study showed that, fruit firmness, titratable acidity, skin brightness and hue angle were not significantly influenced by the treatments. In the same vein, banana fruits when treated only by hydrothermal treatment maintained better postharvest quality trait when compared to the fruits of the hydrothermal treatment associated with calcium chloride. The hydrothermal treatment when together with CaCl2 cans influences in the banana fruit quality affecting skin color and primary metabolism such as sugar and starch content.

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Section: Introductionmentioning

confidence: 99%

The Hydrothermal Treatment Associated with Calcium Chloride Improve Banana cv. ‘Prata Gorutuba’ Quality Modulating Primary Metabolism

Paraizo

Jesus

Aguiar

et al. 2019

AFSJ

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Mitochondrial ascorbate synthesis acts as a pro-oxidant pathway and down-regulate energy supply in plants

Oliveira

Morales

Silva

et al. 2019

Preprint

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31Attempts to improve the ascorbate (AsA) content of plants are still dealing with the limited 32 understanding of why exists a wide variability of this powerful anti-oxidant molecule in different 33 plant sources, species and environmental situations. In plant mitochondria, the last step of AsA 34 synthesis is catalyzed by the enzyme L-galactone-1,4-lactone dehydrogenase (L-GalLDH). By using 35GalLDH-RNAi silencing plant lines, biochemical and proteomic approaches, we here discovered 36 that, in addition to accumulate this antioxidant, mitochondria synthesize AsA to down-regulate the 37 respiratory activity and the cellular energy provision. The work reveals that the AsA synthesis 38 pathway within mitochondria is a branched electron transfer process that channels electrons 39 towards the alternative oxidase, interfering with conventional electron transport. It was 40 unexpectedly found that significant hydrogen peroxide is generated during AsA synthesis, which 41 affects the AsA level. The induced AsA synthesis shows proteomic alterations of mitochondrial 42 and extra-mitochondrial proteins related to oxidative and energetic metabolism. The most 43 identified proteins were known components of plant responses to high light acclimation, 44 programmed cell death, oxidative stress, senescence, cell expansion, iron and phosphorus 45 starvation, different abiotic stress/pathogen attack responses and others. We propose that 46 changing the electron flux associated with AsA synthesis might be part of a new mechanism by 47 which the L-GalLDH enzyme would adapt plant mitochondria to fluctuating energy demands and 48 redox status occurring under different physiological contexts. 49 50 to mETC but via flavin dinucleotide (FAD) (Sweetlove et al., 2010). These oxidation reactions are all 60 coupled to reduction of the ubiquinone (UQ) to ubiquinol (UQH 2 ) (Schertl and Braun, 2014). 61

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Procedures of Mitochondria Purification and Gene Expression to Study Alternative Respiratory and Uncoupling Pathways in Fruits

Cited by 2 publications

References 19 publications

The Hydrothermal Treatment Associated with Calcium Chloride Improve Banana cv. ‘Prata Gorutuba’ Quality Modulating Primary Metabolism

The Hydrothermal Treatment Associated with Calcium Chloride Improve Banana cv. ‘Prata Gorutuba’ Quality Modulating Primary Metabolism

Mitochondrial ascorbate synthesis acts as a pro-oxidant pathway and down-regulate energy supply in plants

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