Procedure Modification of AOAC Official Method 962.17, Volatile Oil in Spices

Ertl, Fran

doi:10.1093/jaoac/80.4.901

Cited by 5 publications

(2 citation statements)

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“…Isolation of Essential Oil. Fresh and dried leaves and commercially available plant material (40 g) were subjected to hydrodistillation until there was no significant increase in the volume of the collected oil (4.5 h) in a Clevenger-type apparatus ( − ). The isolated oils were dried over anhydrous sodium sulfate and stored under N 2 in a sealed vial until required.…”

Section: Methodsmentioning

confidence: 99%

Determination of Monoterpene Hydrocarbons and Alcohols in Majorana hortensis Moench by Micellar Electrokinetic Capillary Chromatographic

Rodrigues

Caramão

Arce

et al. 2002

J. Agric. Food Chem.

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Micellar electrokinetic capillary chromatography was used to determine the essential oils obtained by steam distillation of different samples of marjoram (Majorana hortensis Moench) dried leaves and flowers. The electrophoretic method consisted of a running buffer of 10 mM NaH2PO4, 6 mM Na2B4O7, 50 mM SDS, 7 mM gamma-cyclodextrin, and 10% acetonitrile, adjusted to pH 8.0 by the addition of 0.1 M H3PO4. The following monoterpene hydrocarbons and alcohol compounds were extracted from real samples and determined by the method proposed: alpha-pinene, gamma-terpinene, alpha-terpinene, terpinolene, p-cymene, linalool, alpha-terpineol, and terpinen-4-ol. The most prominent component of dried leaves, flowers, and commercial samples was terpinen-4-ol in four of the samples analyzed; only in one sample was alpha-terpineol present as the major compound.

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Section: Methodsmentioning

confidence: 99%

Determination of Monoterpene Hydrocarbons and Alcohols in Majorana hortensis Moench by Micellar Electrokinetic Capillary Chromatographic

Rodrigues

Caramão

Arce

et al. 2002

J. Agric. Food Chem.

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“…Portanto, considerando estes parâmetros, uma proposta de validação de método cromatográfico, para quantificar α-copaeno, β-cariofileno e α-humuleno, a partir de amostras disponíveis comercialmente foi adicionalmente estabelecida ao presente trabalho. pela AOAC para obter óleos voláteis(ERTL, 1997). Diversos pesquisadores usam esta técnica para obtenção deste voláteis a partir de diferentes fontes vegetais(SILVA et al, 2005).…”

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Copaifera langsdorffii: estudo fitoquímico, validação de métodos cromatográficos e análise sazonal

Sousa¹

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Copaiba refers to large trees that grow mainly in the states of Amazonas, Pará, Ceará and northern Brazil. Copaifera langsdorffii can be found in almost all regions of Brazil. Samples of its oilrresin, especially one obtained from C. langsdorffii have been used in folk medicine as anti-inflammatory, antiseptic, urinary antiseptic and wound healing. In addition, the hydroalcoholic leaves extract of this tree has drawn the attention of our research group because of its antilitiasic properties. Therefore, the goals of this work were: to investigate the chemical profile of samples of C. langsdorffii; to develop and validate chromatographic methods capable of quantifying the main components present in the samples of C. langsdorffii; to evaluate the influence of seasonality in the chemical profile of the ethanolic extracts of this species. For preparation of the extracts maceration and percolation were used. Crude extracts and fractions were analyzed and fractionated by thin layer chromatography and silica gel column chromatography. Promising compounds were purified by HPLC and preparative TLC. The chemical structures of the isolated compounds were elucidated by 1 H NMR and 13 C. The method for the analysis of the hydroalcoholic extract was developed by HPLC/UV-DAD. Also, a protocol for sample preparation was also developed, including shaking, temperature and ethanol 70% as solvent extractor. For the seasonal analysis, aliquots from the leaves of trees were collected monthly for 14 months. GC/MS and GC-FID was used to identify and develop the method, considering the main volatile compounds present in the oil and leaves. For both, Clevenger type apparatus was used to obtain these volatiles. The phytochemical study led to the isolation and identification of 13 compounds. Also, 12 sesquiterpenes were identified, including α-copaene, β-caryophyllene, α-humulene and caryophyllene oxide. Kaurenoic acid was isolated from oilrresin and from the extracts of leaves, flowers and fruits of C. langsdorffii. Copalic acid and its derivatives were obtained by fractionation of the oilrresin. Fractionation of extracts from leaves, flowers and fruits also resulted in the isolation of ent-labda-7,13-dien-15-oic acid and 2-hydroxy-entlabda-7,13-dien-15-oic acid. Two flavonols, 3-O-α-rhamnopyranosyl-quercetin and 3-O-αrhamnopyranosyl-kaempferol were isolated from the hydroalcoholic extracts of the leaves of this species. The 2-hidroxyphenylvinyl ethyl ether was isolated from the extract of the fruits. The some fraction was derivatized furnishing methyl kaurenoate, methyl 2-hydroxy-entlabda-7,13-dien-15-oate and the 4-methoxy-ethyl cinamoate. The developed HPLC method allowed the quantification of five flavonols: rutin, 3-O-α-rhamnopyranosyl-quercetin, 3-O-αrhamnopyranosyl-kaempferol, quercetin and kaempferol. This method showed average recovery of 97%. The qualitative analysis of seasonal variation in the hydroalcoholic extracts of leaves indicated the presence of 3-O-α-rhamnopyranosyl-quercetin and 3-O-αrhamnopyranosyl-kaempferol throughou...

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Composition and Cytotoxicity of Essential Oils from Aerial Parts of Thymus tiflisiensis and T. collinus Growing in Georgia

et al. 2022

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Procedure Modification of AOAC Official Method 962.17, Volatile Oil in Spices

Cited by 5 publications

References 0 publications

Determination of Monoterpene Hydrocarbons and Alcohols in Majorana hortensis Moench by Micellar Electrokinetic Capillary Chromatographic

Determination of Monoterpene Hydrocarbons and Alcohols in Majorana hortensis Moench by Micellar Electrokinetic Capillary Chromatographic

Copaifera langsdorffii: estudo fitoquímico, validação de métodos cromatográficos e análise sazonal

Composition and Cytotoxicity of Essential Oils from Aerial Parts of Thymus tiflisiensis and T. collinus Growing in Georgia

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