Procedure for recording the simultaneous activity of single neurons distributed across cortical areas during sensory discrimination

Abstract

We report a procedure for recording the simultaneous activity of single neurons distributed across five cortical areas in behaving monkeys. The procedure consists of a commercially available microdrive adapted to a commercially available neural data collection system. The critical advantage of this procedure is that, in each cortical area, a configuration of seven microelectrodes spaced 250 -500 m can be inserted transdurally and each can be moved independently in the z axis. For each microelectrode, the data collection system can record the activity of up to five neurons together with the local field potential (LFP). With this procedure, we normally monitor the simultaneous activity of 70 -100 neurons while trained monkeys discriminate the difference in frequency between two vibrotactile stimuli. Approximately 20 -60 of these neurons have response properties previously reported in this task. The neuronal recordings show good signal-to-noise ratio, are remarkably stable along a 1-day session, and allow testing several protocols. Microelectrodes are removed from the brain after a 1-day recording session, but are reinserted again the next day by using the same or different x-y microelectrode array configurations. The fact that microelectrodes can be moved in the z axis during the recording session and that the x-y configuration can be changed from day to day maximizes the probability of studying simultaneous interactions, both local and across distant cortical areas, between neurons associated with the different components of this task.monkeys ͉ multiple microelectrodes ͉ neuronal ensembles ͉ temporal interactions ͉ brain circuits

α-Oscillations in the monkey sensorimotor network influence discrimination performance by rhythmical inhibition of neuronal spiking

“…We simultaneously recorded LFPs and spikes from primary somatosensory cortex (S1), secondary somatosensory cortex (S2), dorsal premotor cortex (DPC), medial premotor cortex (MPC), and primary motor cortex (M1) while a monkey performed a vibrotactile discrimination task (38) (Fig. 1).…”

“…Both spikes and LFPs were recorded simultaneously from five locations in the somatosensory, premotor, and motor cortex (see ref. 38 for details) (Fig. 1B).…”

“…To address these questions, we recorded neuronal activity simultaneously across somatosensory, premotor, and motor cortices in a behaving monkey (38). Both spikes and local field potentials (LFPs) were acquired while the monkey performed a vibrotactile discrimination task (39).…”

Coherent delta-band oscillations between cortical areas correlate with decision making

“…Data acquisition, amplification, and filtering were described in detail (36). In brief, LFPs and the activity of single neurons were simultaneously recorded with an array of seven independent, movable microelectrodes (1-1.5 MΩ) inserted in each of five cortical areas.…”

“…Electrodes within an area were spaced 305 or 500 μm apart (37). Spike sorting was performed manually on-line, and single neurons were selected if they responded to any of the different components of the discrimination task (7,36). The cortical areas were the S1, S2, MPC, DPC, and M1 (Fig.…”