Procedure for incorporation of radioisotopically labeled metabolites into embryos of dipterous insects

Eudy, William W.; Bernheim, Naomi J.; Dobrogosz, Walter J.

doi:10.1016/0003-2697(69)90016-5

Cited by 4 publications

(1 citation statement)

References 6 publications

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“…This is probably due to the difficulty investigators have experienced in introducing foreign substances into the egg. Recently, Eudy et al (1969) have succeeded in introducing labelled uridine and ribonucleic acid (RNA) into dipteran embryos. However, this technique has seen only limited use.…”

Section: Introductionmentioning

confidence: 99%

Yolk-protein degradation during embryogenesis of the African migratory locust

McGregor¹,

Loughton²

1974

Can. J. Zool.

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LOUGHTON. 1974. Yolk-protein de adation during embryogenesis of the African migratory locust. Can. I. Zool. 52: 90f917. An analysis of the individual proteins present during [he r d of locust ernbiyogrneir was undertaken using quantitative paIyacrylamide gel electrop oresis. A total or 13 bands could be resoIvtrt, some of which appeared rlr rrntro during developmenl while others decreased-or disappared. It was round that protein IISC and svnthrsis was a well-regulated process tnuolv~ng pcrlods both of Iigh actwit:, and of relative ~naciivi tt . The periods of most conspicuour rotein chaoger coincided with major n~orphoiugical events: Tlie gariunl degradation of swcrsyof the storage proleins was inrerrupted at spclcitic ttmrs of development when they showed striking increases in concentration. rhe periud when cnrbohjdratrs were used as an energy source could be correlated with the gradual disap earancc of the carbohydrate lmund to the major yolk smraee prolein. A nurnbcr at llarrno&mvmph proteins could he $crnonrtraied in the egg extracts. he ~najor yolk protein was round to be immunologcall tdenlical with the female sex ipecilic blood rotein. Thlr protein frrctlon had properties tkit were ~e r y similar to a Ilpowrellin reporLefin another insect spececles. A drop in rile total soluble protein during early development corrrsponded to a previously reported increase In the free amino acid pool. MCGREGOR, D. A., et B. G. LOUGHTON. 1974. Yolk-protein degradation during embryogenesis of the African migratory locust. Can. J. Zool. 52: 907-917. On a procidt B l'analyse des proteines individuelles prkentes chez le criquet durant l'ernbryogenese, par Plectropllorbe sur gel de pcrlyacrylamjde. On o b s e~e un total de 13 bandes, quelc~ues-anes appnrnlssunr OF rrorn durant le dkveloppcmcnt, alors que d'autres dirnin~icnt ou disparaissmt. On constart: ue I'utiIisation e t la syn~hkse des protgines sc font selon un Frocessus bien rdgle ob 11 y a Its ptriodes de grande activ~tC ct d nulres d'activit8 pratlqucmen1 nulle. LRs p6riodes oil on enregistre les chengements de proteines les plus marquis coincident avec les grandes transformauons morphologiques. La dPgrxdatlon graduetle tie plusicurs des protencs de rCscrve est interrompue 2 des moments spccilirlues du developpement et on =s~slc a des augrnenratiom marquees de leurs concentrations. La pPriode d'utllisation des hydrates de carhone comme source d'inergie e5t en corrPlatlon avec la disparltion grrtduelle de l'hvdmte de carbone reIiC h 1s prot6lne de rtserve pnncipale du vitellus. On a pu dhontrer La presence d'un ceria~n nornbrr de prutCines de I'hernolyrnphe dans drs extraits tl'oeurs. LEI protcinr principale dtl v~tellur est ~dentique. au point dr vue imniunologique, h la protCine du sang slkcifique k la femelle. Cette fraclion proteinique a des prnpntltCs trts sernblables jt Itr Irpovitcll~nc prhsmte c h e~ une autre espkce d'imecte. La haisbe rlu contenu protiinique soluble total, enregistree au tlCbut du dPveIoppclmen~. corres ond B I'augrnentation dejh cli-crite du...

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Section: Introductionmentioning

confidence: 99%

Yolk-protein degradation during embryogenesis of the African migratory locust

McGregor¹,

Loughton²

1974

Can. J. Zool.

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Utilization of amino acids during development of the African migratory locust embryo

McGregor

Loughton

1976

Wilhelm Roux' Archiv

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The metabolic fate of amino acids introduced into the locust egg by the addition of amino acids after oviposition is compared to that of amino acids derived from the maternal haemocoel. It was found that amino acids from the two sources did not always follow the same pattern of utilization. Topical application resulted in some of the applied amino acids being converted into other amino acids and ammonia. When the labelled amino acid was administered to the egg via the maternal haemocoel, radioactivity was restricted to the amino acid orginally provided.

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Ribonucleic acid and enzyme synthesis in developing Phormia regina embryos

Eudy

Dobrogosz

1970

Comparative Biochemistry and Physiology

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Procedure for incorporation of radioisotopically labeled metabolites into embryos of dipterous insects

Cited by 4 publications

References 6 publications

Yolk-protein degradation during embryogenesis of the African migratory locust

Yolk-protein degradation during embryogenesis of the African migratory locust

Utilization of amino acids during development of the African migratory locust embryo

Ribonucleic acid and enzyme synthesis in developing Phormia regina embryos

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