Procalcitonin on the Dusty Way to the Holy Grail: A Progress Report

Müller, Beat

doi:10.1007/0-387-26272-5_40

Cited by 4 publications

(1 citation statement)

References 67 publications

(60 reference statements)

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“…Viral infections: Linscheid and coworkers has shown in differentiated adipocyte cell culture that addition of interleukin-1 beta (IL-1β) has induced the synthesis of PCT while the addition of IL-1β and interferon-gamma (IFN-γ) concomitantly has blocked it [12,13]. In viral infections the host T helper lymphocytes are forced to produce IFN-γ which has a negative feed-back effect on the synthesis of (IL-1β), which is the main trigger of PCT induction.…”

Section: Pct Synthesis In Infectionsmentioning

confidence: 99%

The Molecular Basis of Procalcitonin Synthesis in Different Infectious and Non-Infectious Acute Conditions

Becze

Molnár

Fazakas

2016

JHVRV

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Section: Pct Synthesis In Infectionsmentioning

confidence: 99%

The Molecular Basis of Procalcitonin Synthesis in Different Infectious and Non-Infectious Acute Conditions

Becze

Molnár

Fazakas

2016

JHVRV

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The rapid detection of procalcitonin in septic serum using immunoaffinity MALDI chips

Dvorak,

Novakova,

Kraftova

et al. 2023

Clin Proteom

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Background Sepsis is a common worldwide health condition with high mortality. It is caused by a dysregulated immune response to the pathogen. Severe infections resulting in sepsis can be also determined by monitoring several bloodstream biomarkers, one of them being pro-hormone procalcitonin (PCT). PCT concentration in the bloodstream correlates well with sepsis and in severe cases increases up to a thousand times from the healthy physiological values in a short time. In this study, we developed a rapid technique for PCT detection by MALDI-TOF mass spectrometry, that uses in-situ enrichment directly on the specialized immuno MALDI chips that are utilized as MALDI plates. The method’s ability to detect PCT was confirmed by comparing the results with LC–MS bottom-up workflow. The new method detects intact PCT by its m/z and uncovers its alternations in septic serum. Methods The MALDI chips used for the detection of PCT were prepared by ambient ion soft landing of anti-PCT antibody on an ITO glass slide. The chips were used for the development of the rapid MALDI-TOF MS method. A parallel method based on affinity enrichment on magnetic beads followed by LC–MS/MS data-dependent peptide microsequencing was used to prove PCT presence in the sample. All samples were also tested by ELISA to determine PCT concentration prior to analyzing them by mass spectrometry methods. Results The MALDI chip method was optimized using recombinant PCT spiked into the human serum. The PCT detection limit was 10 ng/mL. The optimized method was used to analyze 13 sera from patients suffering sepsis. The PCT results were confirmed by LC–MS/MS. The measurement of the intact PCT by the MALDI chip method revealed that sera of patients with severe sepsis have other forms of PCT present, which show post-processing of the primary sequence by cleavage of PCT, resulting in the formation of N and C termini fragments. Conclusions Procalcitonin from human serum was successfully enriched and detected using immunoaffinity MALDI chips. The intact PCT was characterized in 13 septic patients. The method is more specific compared to non-MS-based immunoaffinity techniques and allows observation of different variants of PCT in septic patients.

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Multiple organ failure: early diagnosis and prognosis

Радивилко¹,

Grigoryev²,

Шукевич³

et al. 2018

Anesteziologiya I Reanimatologiya

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ФГБНУ «Научно-исследовательский институт комплексных проблем сердечно-сосудистых заболеваний», 650002, Кемерово, Россия Полиорганная недостаточность (ПОН)-универсальный синдром критических состояний, вызванный чрезмерным неконтролируемым системным воспалением в ответ на сепсис, гиповолемический шок, искусственное кровообращение. Несмотря на внедрение шкал оценки тяжести ПОН, которые включают распространенные в широкой клинической практике параметры инструментального и биохимического мониторинга, диагностическое и прогностическое значение подобных шкал далеко от совершенства. Использование биохимических маркеров ПОН является наиболее перспективным в отношении расширения возможностей оценки тяжести и прогноза ПОН. В обзоре обсуждается внедрение новых биохимических маркеров в качестве прикроватных методов диагностики ПОН. Ключевые слова: полиорганная недостаточность, биомаркеры, прогноз, сепсис, синдром системного воспалительного ответа. Для корреспонденции: Радивилко Артем Сергеевич-к.м.н., старший научный сотрудник лаборатории критических состояний ФГБНУ «Научно-исследовательский институт комплексных проблем сердечно-сосудистых заболеваний»,

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Procalcitonin on the Dusty Way to the Holy Grail: A Progress Report

Cited by 4 publications

References 67 publications

The Molecular Basis of Procalcitonin Synthesis in Different Infectious and Non-Infectious Acute Conditions

The Molecular Basis of Procalcitonin Synthesis in Different Infectious and Non-Infectious Acute Conditions

The rapid detection of procalcitonin in septic serum using immunoaffinity MALDI chips

Multiple organ failure: early diagnosis and prognosis

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