The specimens of the genus Cobboldina Leiper, 1911 and family Atractidae (Railliet, 1917) Travassos, 1919 recovered from the faecal matter of Hippopotamus (Hippopotamus amphibius Linnaeus, 1758) from the Alipore Zoological Garden, Kolkata, India recognized as a new species after careful observation. The collected nematode differs from the only valid species Cobboldina vivipara Leiper, 1911, in the presence of gubernaculum (13.2-29.7 lm in length) and the number of caudal papillae (10 pairs) and named as Cobboldina gubernacularia sp. n., This is the second species of the genus Cobboldina recorded from the host Hippopotamus amphibius Linnaeus, 1758 remaining captive in the Alipore Zoological Garden, Kolkata, India.