Problems of rabies surveillance and control in the Russian Federation

Симонова, Е. Г.; Zubareva, K.Yu.; Kartavaya, S.A.; Раичич, С. Р.; Shabeykin, A.A.; Gulukin, A.M.

doi:10.20953/1729-9225-2018-3-31-36

Cited by 3 publications

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“…into the cells of peripheral nervous system. It is considered that, at the place of penetration into organism, rabies virus can replicate in muscle cells as well as virion direct penetration, without preliminary replication, into nervous cells is possible [10][11][12]. Nevertheless, on all of the early stages of organism contamination, rabies virus uses masking strategy from immune system and his replication stays on minimal level during all of this period.…”

Section: Introductionmentioning

confidence: 99%

“…From this moment, virus particles can be disclosed by laboratory methods in organism different tissues and liquids such as saliva, smear preparation from eye cornea, skin biopsy samples [17]. Because of quickly flowing terminal stage, express-methods such as fluorescent spectroscopy [11] as well as Reverse Transcription Polymerase Chain Reaction (RT-PCR) [17] are more vital for rabies intravital diagnostics.…”

Section: Introductionmentioning

confidence: 99%

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Intravital and postmortem laboratory diagnostics of hydrophobia clinical cases

et al. 2021

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Rabies excitant is a neurophilic virus (Rabiesvirus), regarding to Mononegavirales order, Rhabdoviridae family, Lyssavirus genus. The situation with rabies morbidity in Russia is characterized as extremely unpleasant. Saliva, tear liquid, patient’s cornea prints were used in the work as well as, after their death, various segments of brain and salivary glands were used. Additionally, antibody concentration in blood serum of humans passing antirabies therapy after animal bites were investigated. Research Research of eye cornea prints from patient A, suffered from wolf bite, with antibody fluorescent method showed positive result 6 days before the death. The obtained results were confirmed postmortem while the investigation of brain various segments and salivary glands with the use of antibody fluorescent spectroscopy and enzyme-linked analysis method, ELISA, and in biosamples. Intravital research of various biosamples picked from the patient B, suffered from homeless dog bite, showed the precedence of Virus 9 days before the death. Rabies virus antigen was discovered on the basis of research results of eye cornea prints with confocal spectroscopy, saliva samples - with ELISA method, as well as rabies virus genome in saliva and tear liquid was demonstrated with Nested Enzyme-Linked Analysis with Reverse Transcription (Nested RT-PCR). Intravital diagnostics results of hydrophobia patient were confirmed afterwards postmortem while the investigation of brain various segments with confocal fluorescence spectroscopy, ELISA, method and biopsy. Hydrophobia of injured was going on in clinically well-expressed manner. The results of intravital and postmortem laboratory diagnostics of hydrophobia clinical cases in Russian Federation were considered. These cases were the result of wolf and homeless dog bites. Both hydrophobia cases, caused by wild predator and dog bite, were laboratory confirmed yet during patient’s life. Introduction of intravital laboratory rabies diagnostics into medical practice is an important element of differential diagnostics.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Intravital and postmortem laboratory diagnostics of hydrophobia clinical cases

et al. 2021

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Synthetic oligonucleotide primers to glycoprotein gene of rabies virus and based by nested polymerase chain reaction method for RNA Rabies virus detection

Gulyukin¹,

Shabeykin²,

Белименко³

et al. 2020

IOP Conf. Ser.: Earth Environ. Sci.

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One of widely used methods to detect rabies virus RNA is a reverse transcription chain polymerase reaction (RT-PCR) allowing to determine a diagnosis in 5 hours. Thus, we have elaborated the method that allows to pursue effective elicitation of RNA strains and isolates of rabies virus in pathological and clinical material as well as to decrease research timespan to 6 hours, to lower by 9.8 times the diagnostics prime cost, labor expenditures – by 40 times. The way includes performance of nested RT-PCR with oligonucleotide primers having definite nucleotide sequences and synthesized to conservative glycoprotein gene. Wherein RT-PCR is pursued in two rounds. In the case of positive reaction, fragment corresponding to the size in the first round – 755 bp, in the second round – 259 bp, is synthesized.

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Molecular characteristics isolates of rabies virus isolated from humans in Ukraine

Polupan¹,

Недосеков

Степанова

et al. 2021

IOP Conf. Ser.: Earth Environ. Sci.

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The article presents the results of molecular-genetic investigation of isolates of rabies virus was isolated from 2 rabies patients in Ukraine. It has been confirmed that the virus that caused the deaths belongs to animal lyssavirus, namely the rabies virus. It has been proved that street isolates from humans by their genetic characteristics belong to the first genotype (RABV), the first phylogroup of animal lyssavirus. High homology between experimental samples and materials from cats, dogs, and foxes from the territory of Ukraine and with isolates in fox populations in South-Eastern Europe was revealed. RT-PCR confirmed that 2 people died of rabies, the isolates belong to the first phylogroup of lissavirus, the first genotype (RABV). It is proved that 2 street isolates from people from Ukraine are similar in degree of homology to the isolates isolated in fox populations in South-Eastern Europe.

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Problems of rabies surveillance and control in the Russian Federation

Cited by 3 publications

References 0 publications

Intravital and postmortem laboratory diagnostics of hydrophobia clinical cases

Intravital and postmortem laboratory diagnostics of hydrophobia clinical cases

Synthetic oligonucleotide primers to glycoprotein gene of rabies virus and based by nested polymerase chain reaction method for RNA Rabies virus detection

Molecular characteristics isolates of rabies virus isolated from humans in Ukraine

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