Problems in the Analysis of Urinary Coproporphyrin III

Effects on the Coproporphyrin Test of Drugs Used in the Treatment of Parasitic Disease

“…The methods mainly used for the quantitative determination of coproporphyrin fall into two groups: fluorimetric (De Langen and ten Berg, 1948;Fikentscher, 1932;Harrold, Meek, and Padden, 1952;Johnson and Whitman, 1950;Maloof, 1950;Parkinson and Cholak, 1952;Schwartz, Hawkinson, Cohen, and Watson, 1947;Schwartz, Zieve, and Watson, 1951) and, less frequently, spectrophotometric (Sveinsson, Rimington, and Barnes, 1949). Practically all the fluorimetric methods described in the literature are based on the extraction of coproporphyrin from acidified urine by means of ether or ethyl acetate and its subsequent further extraction from the organic solvent with hydrochloric acid.…”