Problems in electronic state assignment based on circular dichroism. Optical activity of flavines and 8-substituted lumazines

Harders, H; Förster, Sebastian; Voelter, Wolfgang; Bacher, A.

doi:10.1021/bi00713a028

Cited by 25 publications

(15 citation statements)

References 27 publications

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“…Figure 2 shows the CD spectrum of VB 2 in PBS (pH 6.8) in a conventional 1-cm CD cell. The CD peaks appear at 455, 345, 270, 220 nm, approximately, which are consistent with the cotton bands reported previously [26]. The spectrum obtained may be interpreted by interactions of the side chain centers of chirality with the flavin chromphore (isoalloxazine ring).…”

Section: Resultssupporting

confidence: 90%

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Circular Dichroism Spectroelectrochemical and Voltammetric Studies of Vitamin B2

et al. 2001

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Section: Resultssupporting

confidence: 90%

“…It is essential for the understanding of the optical properties of flavoprotein complexes [26]. CD SEC and voltammetric studies also provided us some electrochemical and adsorption information in the electroreduction process.…”

Section: Introductionmentioning

confidence: 99%

Circular Dichroism Spectroelectrochemical and Voltammetric Studies of Vitamin B2

et al. 2001

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“…The pathway intermediate, 6,7-dimethyl-8-ribityllumazine, differs structurally from the flavocoenzymes by the absence of the benzenoid ring. Despite that, the redox properties of 8-substituted pteridines and 10-substituted isoalloxazine-type flavocoenzymes are similar (50). It is therefore conceivable that lumazine-type coenzymes could have preceded present day flavocoenzymes.…”

Section: Discussionmentioning

confidence: 99%

Biosynthesis of Vitamin B2

Illarionov

Eisenreich

Schramek

et al. 2005

Journal of Biological Chemistry

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The dismutation of 6,7-dimethyl-8-ribityllumazine catalyzed by riboflavin synthase affords riboflavin and 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione. A pentacyclic adduct of two 6,7-dimethyl-8-ribityllumazines has been identified earlier as a catalytically competent reaction intermediate of the Escherichia coli enzyme. Acid quenching of reaction mixtures of riboflavin synthase of Methanococcus jannaschii, a paralog of 6,7-dimethyl-8-ribityllumazine synthase devoid of similarity with riboflavin synthases of eubacteria and eukaryotes, afforded a compound whose optical absorption and NMR spectra resemble that of the pentacyclic E. coli riboflavin synthase intermediate, whereas the circular dichroism spectra of the two compounds have similar envelopes but opposite signs. Each of the compounds could serve as a catalytically competent intermediate for the enzyme by which it was produced, but not vice versa. All available data indicate that the respective pentacyclic intermediates of the M. jannaschii and E. coli enzymes are diastereomers.

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“…The spectral location of these transitions is, however, not fully known. Some authors suggest their appearance at 300 nm, 370 nm or 480 nm (Song 1971;Harders, Förster, Voelter& Bacher, 1974, Drabent,1979. The location of transition moments in absorption and the angle between them has also been investigated and determined among others by Johanssson et al (Johansson, Davidson, Lindblom & Naqvi; and Eaton et al (Eaton, Hofrichter, Makinen, Andersen & Ludwig, 1975 Heelis (1982), Drabent (1977) and Grajek (2007).…”

Section: Spectral and Structural Properties Of Flavin Photoreceptorsmentioning

confidence: 99%

Review - Flavins as photoreceptors of blue light and their spectroscopic properties

Grajek¹

2011

Current Topics in Biophysics

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This review describes 1) the development of studies on flavin photoreceptors as blue light photoreceptors in many living organisms: their kinds and functions; 2) the studies on spectroscopic properties of flavins, both their dimers and monomers; 3) nonradiative excitation energy transport in the presence of monomers and fluorescent/nonflurescent FMN dimers (excitation traps). The existence equilibrated luminescent FMN centers, energy migration and excitation sink to FMN dimers are taken into account.

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Problems in electronic state assignment based on circular dichroism. Optical activity of flavines and 8-substituted lumazines

Cited by 25 publications

References 27 publications

Circular Dichroism Spectroelectrochemical and Voltammetric Studies of Vitamin B2

Circular Dichroism Spectroelectrochemical and Voltammetric Studies of Vitamin B2

Biosynthesis of Vitamin B2

Review - Flavins as photoreceptors of blue light and their spectroscopic properties

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