Problems and possibilities of chemistry on dry reagent carriers

Greyson, Jerome

doi:10.1155/s1463924681000199

Cited by 23 publications

(5 citation statements)

References 2 publications

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“…The larger SEE for the dry chemistry method can probably be ascribed to a higher sensitivity to matrix effects (16). The precision for creatine kinase-MB is consistent with earlier investigations (3,5,10,11,17).…”

Section: Discussionsupporting

confidence: 86%

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Comparison of Two Immunoinhibitory Methods with Agarose Gel-Electrophoresis for Measuring the MB Isoenzyme of Creatine Kinase in Serum from Cases of Suspected Myocardial Infarction

Hadberg¹,

Hassager²,

Hildebrandt³

et al. 1990

Clinical Chemistry and Laboratory Medicine

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Summary:Two immunoinhibitory methods for measuring creatine kinase-MB (a dry chemical and a wet chemical method) were compared with the commonly used agarose gel electrophoretic method, using 563 serum samples from 235 patients with suspected acute myocardial infarction. Comparison of the electrophoretic and the dry chemistry methods showed the linear relationship: electrophoretic method = -6.5 U/l + 1.22 χ dry chemistry method, r = 0.943. For the wet chemistry method the relationship was: electrophoretic method = -7.2 U/l + 1.19 x wet chemistry method, r = 0.854. Parallel determinations of total creatine kinase were also done and the methods were virtually identical in performance.Compared with the electrophoretic method (which showed a 15% prevalence of acute myocardial infarction), these classifications showed sensitivities of 0.92 and 0.67 and specificities of 0.94 and 0.99 (dry and wet chemistry, respectively; using methods recommended by the manufacturers). After optimization of discriminators the sensitivity was increased to 0.94/0.92 and the specificity to 0.99/0.99. Introduction." ., ,. . , specific antibodies against the subumt M. The antiAcute myocardial infarction is one of the commonest bodies have either been used in radioimmunoassays causes of hospitalization and death in the western or for specific inhibition of the enzymatic activity of industrialized countries. It is therefore of great im-the subunit M, followed by measurement of the reportance to have a fast, inexpensive, sensitive, and maining enzymatic activity (2, 5). The aim of this specific diagnostic test. The most widely used single study is to compare two different immunoinhibition laboratory test is electrophoretic determination of the methods (a dry and a wet chemistry method) with the MB isoenzyme of creatine kinase (EC 2.7.3.2) 1 ) (crea-commonly used but laborious electrophoretic method, tine kinase-MB) (1, 2). This method is very specific and reasonably sensitive, but it is rather tedious and expensive (3, 4). The last decade has seen the devel-Materials and Methods opment of easier analytical methods, which utilize Samples

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Section: Discussionsupporting

confidence: 86%

“…The accuracy of the methods was estimated by comparison with the electrophoretic method; various lyophilized materials with assigned values were inappropriate for analysis by the dry chemistry method, because their viscosity differed from that of serum (16).…”

Section: Discussionmentioning

confidence: 99%

Comparison of Two Immunoinhibitory Methods with Agarose Gel-Electrophoresis for Measuring the MB Isoenzyme of Creatine Kinase in Serum from Cases of Suspected Myocardial Infarction

Hadberg¹,

Hassager²,

Hildebrandt³

et al. 1990

Clinical Chemistry and Laboratory Medicine

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show abstract

“…The Reflectometer output the results in 2-3 minutes without requiring much effort. So, this technology has a more advantage whenever there is no specialized clinical pathologist and no rapid transport System [5][6][7][8].…”

Section: Dry Chemistrymentioning

confidence: 99%

Therapeutic Drug Monitoring and Its Analytical Methods - An Educational Review

2022

MCMS

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“…The solid components such as fibres scatter part of the light and the liquid component, which contains the coloured reaction products, absorbs part of the light both as it passes into the matrix and as it is reflected out of it. To make the measurement of reflected light more useful several algorithms are available to convert reflectance measurements to functions that are linear with respect to analyte concentration (Greyson 1981). The particular algorithm employed depends on the nature of the illumination, the reflection characteristics of the reagent carrier and the optical geometry of the instrument.…”

Section: Dry-reagent Analysersmentioning

confidence: 99%

Asymptotic behaviour of the fundamental solution of a second-order parabolic equation

Lelikova¹

1995

Sb. Math.

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Problems and possibilities of chemistry on dry reagent carriers

Cited by 23 publications

References 2 publications

Comparison of Two Immunoinhibitory Methods with Agarose Gel-Electrophoresis for Measuring the MB Isoenzyme of Creatine Kinase in Serum from Cases of Suspected Myocardial Infarction

Comparison of Two Immunoinhibitory Methods with Agarose Gel-Electrophoresis for Measuring the MB Isoenzyme of Creatine Kinase in Serum from Cases of Suspected Myocardial Infarction

Therapeutic Drug Monitoring and Its Analytical Methods - An Educational Review

Asymptotic behaviour of the fundamental solution of a second-order parabolic equation

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