Problematic Crops: 1. Potatoes

Jo, Kwang‐Ryong; Zhu, Suxian; Bai, Yuling; Hutten, R.C.B.; Kessel, G.J.T.; Vleeshouwers, Vivianne G. A. A.; Jacobsen, E.; Visser, Richard G. F.; Vossen, Jack H.

doi:10.1002/9781118867716.ch9

Cited by 10 publications

(6 citation statements)

References 81 publications

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“…2012 ). Marker-assisted breeding is then considered efficient for breeding, although R gene activity by functional effector assays seems the best method to distinguish between mechanistically different R genes ( Vleeshouwers et al., 2011b , Jo et al., 2016 ). In this study however, we show that R genes that recognize the same effector (AVR2) can still confer different resistance patterns, which further nuances the strategy to discriminate race-specificity of R genes.…”

Section: Discussionmentioning

confidence: 99%

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Two different R gene loci co-evolved with Avr2 of Phytophthora infestans and confer distinct resistance specificities in potato

Aguilera-Gálvez

Champouret

Rietman

et al. 2018

Studies in Mycology

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Late blight, caused by the oomycete pathogen Phytophthora infestans, is the most devastating disease in potato. For sustainable management of this economically important disease, resistance breeding relies on the availability of resistance (R) genes. Such R genes against P. infestans have evolved in wild tuber-bearing Solanum species from North, Central and South America, upon co-evolution with cognate avirulence (Avr) genes. Here, we report how effectoromics screens with Avr2 of P. infestans revealed defense responses in diverse Solanum species that are native to Mexico and Peru. We found that the response to AVR2 in the Mexican Solanum species is mediated by R genes of the R2 family that resides on a major late blight locus on chromosome IV. In contrast, the response to AVR2 in Peruvian Solanum species is mediated by Rpi-mcq1, which resides on chromosome IX and does not belong to the R2 family. The data indicate that AVR2 recognition has evolved independently on two genetic loci in Mexican and Peruvian Solanum species, respectively. Detached leaf tests on potato cultivar ‘Désirée’ transformed with R genes from either the R2 or the Rpi-mcq1 locus revealed an overlapping, but distinct resistance profile to a panel of 18 diverse P. infestans isolates. The achieved insights in the molecular R – Avr gene interaction can lead to more educated exploitation of R genes and maximize the potential of generating more broad-spectrum, and potentially more durable control of the late blight disease in potato.

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Section: Discussionmentioning

confidence: 99%

“…Therefore, the modern breeding approach is to isolate a variation of R genes and deploy them in pyramids. This is expected to lead to broad-spectrum recognition of P. infestans isolates and might provide a more durable resistance ( Jo et al. 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Two different R gene loci co-evolved with Avr2 of Phytophthora infestans and confer distinct resistance specificities in potato

Aguilera-Gálvez

Champouret

Rietman

et al. 2018

Studies in Mycology

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show abstract

“…Currently breeders isolate variants of R genes and deploy them in pyramids or stacks. It is expected that this will result in broad-spectrum recognition of Pi isolates and might provide a more durable resistance in the field (Jo et al, 2016). Recent screening of germplasm has revealed Rpi genes in wild species of Solanum, particularly broadspectrum resistance genes in blb species (Wang et al, 2008(Wang et al, , 2013Hein et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Introgression of Two Broad-Spectrum Late Blight Resistance Genes, Rpi-Blb1 and Rpi-Blb3, From Solanum bulbocastanum Dun Plus Race-Specific R Genes Into Potato Pre-breeding Lines

et al. 2020

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Potato Late Blight Resistance Genes of the parent cultivar 'Delikat' in field trials. The evaluation of agronomic traits of selected BC 2 clones and of their processing qualities revealed valuable material for breeding late blight durable resistant potato. We show that the combination of somatic hybridization with the additional use of gene specific markers and corresponding Avr effectors is an efficient approach for the successful identification and introgression of late blight resistance genes into the potato gene pool.

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“…The Rpi‐ber1.1 and Rpi‐tar1.1 genes were amplified using primers in the promotor and terminator. The resulting PCR fragments were cloned into pBINPLUS‐PASSA (Jo et al , 2016 ) and were expressed in transgenic Desiree plants under the control of their native regulatory elements. For transient expression analyses, the coding sequences of the allelic variants were cloned under the Rpi‐chc1.1 regulatory elements (900‐bp promotor and 400‐bp terminator) into pDEST using a multisite gateway protocol.…”

Section: Methodsmentioning

confidence: 99%

Allelic variants of the NLR protein Rpi‐chc1 differentially recognize members of the Phytophthora infestans PexRD12/31 effector superfamily through the leucine‐rich repeat domain

et al. 2021

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Phytophthora infestans is a pathogenic oomycete that causes the infamous potato late blight disease. Resistance (R) genes from diverse Solanum species encode intracellular receptors that trigger effective defense responses upon the recognition of cognate RXLR avirulence (Avr) effector proteins. To deploy these R genes in a durable fashion in agriculture, we need to understand the mechanism of effector recognition and the way the pathogen evades recognition. In this study, we cloned 16 allelic variants of the Rpi-chc1 gene from Solanum chacoense and other Solanum species, and identified the cognate P. infestans RXLR effectors. These tools were used to study effector recognition and co-evolution. Functional and non-functional alleles of Rpi-chc1 encode coiled-coil nucleotide-binding leucine-rich repeat (CNL) proteins, being the first described representatives of the CNL16 family. These alleles have distinct patterns of RXLR effector recognition. While Rpi-chc1.1 recognized multiple PexRD12 (Avrchc1.1) proteins, Rpi-chc1.2 recognized multiple PexRD31 (Avrchc1.2) proteins, both belonging to the PexRD12/31 effector superfamily. Domain swaps between Rpi-chc1.1 and Rpi-chc1.2 revealed that overlapping subdomains in the leucine-rich repeat (LRR) domain are responsible for the difference in effector recognition. This study showed that Rpi-chc1.1 and Rpi-chc1.2 evolved to recognize distinct members of the same PexRD12/31 effector family via the LRR domain. The biased distribution of polymorphisms suggests that exchange of LRRs during host-pathogen co-evolution can lead to novel recognition specificities. These insights will guide future strategies to breed durable resistant varieties.

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Problematic Crops: 1. Potatoes

Cited by 10 publications

References 81 publications

Two different R gene loci co-evolved with Avr2 of Phytophthora infestans and confer distinct resistance specificities in potato

Two different R gene loci co-evolved with Avr2 of Phytophthora infestans and confer distinct resistance specificities in potato

Introgression of Two Broad-Spectrum Late Blight Resistance Genes, Rpi-Blb1 and Rpi-Blb3, From Solanum bulbocastanum Dun Plus Race-Specific R Genes Into Potato Pre-breeding Lines

Allelic variants of the NLR protein Rpi‐chc1 differentially recognize members of the Phytophthora infestans PexRD12/31 effector superfamily through the leucine‐rich repeat domain

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