Probiotics modify body weight together with anxiety states via pro-inflammatory factors in HFD-treated Syrian golden hamster

Avolio, Ennio; Fazzari, Gilda; Zizza, Merylin; Lorenzo, Antonino De; Renzo, Laura Di; Alò, Raffaella; Facciolo, Rosa Maria; Canonaco, Marcello

doi:10.1016/j.bbr.2018.09.010

Cited by 39 publications

(27 citation statements)

References 55 publications

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“…In animals subjected to 21 days of restraint stress, Lactobacillus helveticus NS8 supplementation increased time spent in the open arms in EPM [14]. Similarly, a recent study shows that animals that received a probiotics treatment exhibited anxiolytic-like behaviours after exposure to an unpredictable chronic mild stress (UCMS) test for four weeks [68]. Moreover, several clinical studies have demonstrated significant health benefits after consumption of probiotics, including decreased anxiety ratings and a lowered stress response in healthy volunteers, reinforcing the benefits of probiotic supplementation in the treatment of mood- and stress-related diseases [69,70,71].…”

Section: Resultsmentioning

confidence: 99%

In Vitro Probiotic and Antioxidant Potential of Lactococcus lactis subsp. cremoris LL95 and Its Effect in Mice Behaviour

et al. 2019

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The composition of intestinal microbiota is widely believed to not only affect gut health but also influence behaviour. This study aimed to evaluate the probiotic characteristics, antioxidant activity, and antidepressant- and anxiolytic-like activities of Lactococcus lactis subsp. cremoris LL95. This strain showed probiotic properties such as resistance in a simulated gastric tract model and survival at different concentrations of NaCl and bile salts. Moreover, antioxidant activity of LL95 was demonstrated through DPPH radical scavenging activity, scavenging of ABTS•+ radical and ferric ion reducing antioxidant power (FRAP) assays. Female C57BL/6 mice received LL95 orally at a dose of 109 UFC/day for 28 days. LL95 improved depressive- and anxiety-like behaviour, demonstrated by decreased immobility time in the tail suspension test and forced swim test and increased per cent of time spent in the open arms on the elevated plus maze. These findings indicate the potential antioxidant activity of LL95 and its role in behaviour, suggesting that probiotic may have therapeutic applications.

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Section: Resultsmentioning

confidence: 99%

In Vitro Probiotic and Antioxidant Potential of Lactococcus lactis subsp. cremoris LL95 and Its Effect in Mice Behaviour

et al. 2019

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“…These tests were conducted to confirm that there were no differences in behavioral parameters between each group. The six-week uCMS was a slightly modified variation of the procedure previously described by other researchers [47,48,49,50]. Control and RU486 rats were housed together with the uCMS and uCMS + RU486 rats.…”

Section: Methodsmentioning

confidence: 99%

Mechanisms of Stress-Induced Spermatogenesis Impairment in Male Rats Following Unpredictable Chronic Mild Stress (uCMS)

Zou

Wang

et al. 2019

IJMS

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The negative association between psychological stress and male fertility has been known for many years. This study was aimed at (i) identifying spermatogenesis impairment induced by psychological stress in rats and (ii) exploring the role of glucocorticoid receptor (GR) signaling in these adverse effects (if they exist). Male Sprague Dawley rats were exposed to a six-week period of unpredictable chronic mild stress (uCMS) along with cotreatment of GR antagonist RU486 (1 mg/kg/day). Testicular damage was assessed by testicular pathological evaluation, epididymal sperm concentration, serum testosterone levels, testicular apoptotic cell measurements, and cell cycle progression analyses. Rats in the uCMS group had decreased levels of serum testosterone and decreased epididymal sperm concentration. The uCMS-treated rats also had decreased numbers of spermatids and increased levels of apoptotic seminiferous tubules; additionally, cell cycle progression of spermatogonia was arrested at the G0/G1 phase. Furthermore, uCMS exposure caused an increase in serum corticosterone level and activated GR signaling in the testes including upregulated GR expression. RU486 treatment suppressed GR signaling and alleviated the damaging effects of stress, resulting in an increased epididymal sperm concentration. Overall, this work demonstrated for the first time that the activation of GR signaling mediates stress-induced spermatogenesis impairment and that this outcome is related to cell apoptosis and cell cycle arrest in germ cells.

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“…The fact that placebo-treated rats reared more with support than probiotic-treated rats is noteworthy, as probiotic treatment is reported to improve anxiety in animal studies [ 18 , 24 ] and decrease the stress response [ 15 , 16 , 62 , 63 ]. Rearing is a complex behaviour (especially supported rearing) [ 45 ], and should be interpreted with caution as the act of performing a supported rear can vary based on the state of the animal performing the behaviour (i.e., escape behaviour or exploratory behaviour) [ 61 ].…”

Section: Discussionmentioning

confidence: 99%

“…In fact, probiotic animals displayed higher levels of pro-inflammatory analyte, IL-1α, but also of the anti-inflammatory analyte, IL-10, and the regulatory analytes, M-CSF and MIP-3α. Prior studies have shown that probiotic treatment decreases pro-inflammatory analytes and factors in response to chronic stress (e.g., IL-6 in rats [ 23 ]; IL-1β and NF-κB in Syrian golden hamsters [ 24 ]). Furthermore, Dai and colleagues [ 79 ] administered a probiotic including Bifidobacteria , Lactobacilli , and one Streptococcus species in a rat model of colitis and found that treatment similarly induced production of IL-10 (anti-inflammatory), but concurrently, reduced production of pro-inflammatory analytes (e.g., TNF-α and IL-6 in colon and serum).…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, specific probiotic formulations have been shown to decrease the response to stress in rodent models (e.g., Companilactobacillus farciminis [ 15 ]; CEREBIOME ® [ 16 ]; Lacidofil ® [ 17 ]), improve anxiety symptomatology in humans and anxiety-like behaviours in rats (e.g., CEREBIOME ® [ 18 ]), improve obesogenic outcomes (e.g., Lactiplantibacillus plantarum [ 19 ]; Bifidobacterium spp. [ 20 , 21 ]; L. helveticus R0052 [ 22 ]), and reduce the inflammatory response (e.g., B. infantis 35624 [ 23 ]; 8-strain combination probiotic [ 24 ]; 3-strain combination probiotic [ 25 ]).…”

Section: Introductionmentioning

confidence: 99%

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Supplementation with Combined Lactobacillus helveticus R0052 and Bifidobacterium longum R0175 Across Development Reveals Sex Differences in Physiological and Behavioural Effects of Western Diet in Long–Evans Rats

et al. 2020

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The gut microbiome affects various physiological and psychological processes in animals and humans, and environmental influences profoundly impact its composition. Disorders such as anxiety, obesity, and inflammation have been associated with certain microbiome compositions, which may be modulated in early life. In 62 Long–Evans rats, we characterised the effects of lifelong Bifidobacterium longum R0175 and Lactobacillus helveticus R0052 administration—along with Western diet exposure—on later anxiety, metabolic consequences, and inflammation. We found that the probiotic formulation altered specific anxiety-like behaviours in adulthood. We further show distinct sex differences in metabolic measures. In females, probiotic treatment increased calorie intake and leptin levels without affecting body weight. In males, the probiotic seemed to mitigate the effects of Western diet on adult weight gain and calorie intake, without altering leptin levels. The greatest inflammatory response was seen in male, Western-diet-exposed, and probiotic-treated rats, which may be related to levels of specific steroid hormones in these groups. These results suggest that early-life probiotic supplementation and diet exposure can have particular implications on adult health in a sex-dependent manner, and highlight the need for further studies to examine the health outcomes of probiotic treatment in both sexes.

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Probiotics modify body weight together with anxiety states via pro-inflammatory factors in HFD-treated Syrian golden hamster

Cited by 39 publications

References 55 publications

In Vitro Probiotic and Antioxidant Potential of Lactococcus lactis subsp. cremoris LL95 and Its Effect in Mice Behaviour

In Vitro Probiotic and Antioxidant Potential of Lactococcus lactis subsp. cremoris LL95 and Its Effect in Mice Behaviour

Mechanisms of Stress-Induced Spermatogenesis Impairment in Male Rats Following Unpredictable Chronic Mild Stress (uCMS)

Supplementation with Combined Lactobacillus helveticus R0052 and Bifidobacterium longum R0175 Across Development Reveals Sex Differences in Physiological and Behavioural Effects of Western Diet in Long–Evans Rats

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