Probiotics consortium synergistically ameliorates aflatoxin B1-induced disruptions in lipid metabolism of female albino rats

Ugbaja, Regina Ngozi; Okedairo, Oluwasegun Micheal; Oloyede, A. R.; Ugwor, Emmanuel Ifeanyichukwu; Akinloye, Dorcas Ibukun; Ojo, Olusola Peter; Ademuyiwa, Oladipo

doi:10.1016/j.toxicon.2020.08.007

Cited by 11 publications

(6 citation statements)

References 42 publications

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“…The decrease in PC32:1, PE, and DAG may be accompanied by inflammation. Previous research showed that AFB1 disrupted intestinal homeostasis, which was marked by a reduction in TAG and cholesterol [76]. Another study pointed out that the plasma and liver levels of TAG in rats were increased after acute exposure to AFB1 [69].…”

Section: Discussionmentioning

confidence: 98%

Integrated Metabolomics and Lipidomics Analysis Reveals Lipid Metabolic Disorder in NCM460 Cells Caused by Aflatoxin B1 and Aflatoxin M1 Alone and in Combination

Yang

Li²,

Gao

et al. 2023

Toxins

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Aflatoxin B1 (AFB1) and aflatoxin M1 (AFM1) are universally found as environmental pollutants. AFB1 and AFM1 are group 1 human carcinogens. Previous sufficient toxicological data show that they pose a health risk. The intestine is vital for resistance to foreign pollutants. The enterotoxic mechanisms of AFB1 and AFM1 have not been clarified at the metabolism levels. In the present study, cytotoxicity evaluations of AFB1 and AFM1 were conducted in NCM 460 cells by obtaining their half-maximal inhibitory concentration (IC50). The toxic effects of 2.5 μM AFB1 and AFM1 were determined by comprehensive metabolomics and lipidomics analyses on NCM460 cells. A combination of AFB1 and AFM1 induced more extensive metabolic disturbances in NCM460 cells than either aflatoxin alone. AFB1 exerted a greater effect in the combination group. Metabolomics pathway analysis showed that glycerophospholipid metabolism, fatty acid degradation, and propanoate metabolism were dominant pathways that were interfered with by AFB1, AFM1, and AFB1+AFM1. Those results suggest that attention should be paid to lipid metabolism after AFB1 and AFM1 exposure. Further, lipidomics was used to explore the fluctuation of AFB1 and AFM1 in lipid metabolism. The 34 specific lipids that were differentially induced by AFB1 were mainly attributed to 14 species, of which cardiolipin (CL) and triacylglycerol (TAG) accounted for 41%. AFM1 mainly affected CL and phosphatidylglycerol, approximately 70% based on 11 specific lipids, while 30 specific lipids were found in AFB1+AFM1, mainly reflected in TAG up to 77%. This research found for the first time that the lipid metabolism disorder caused by AFB1 and AFM1 was one of the main causes contributing to enterotoxicity, which could provide new insights into the toxic mechanisms of AFB1 and AFM1 in animals and humans.

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Section: Discussionmentioning

confidence: 98%

Integrated Metabolomics and Lipidomics Analysis Reveals Lipid Metabolic Disorder in NCM460 Cells Caused by Aflatoxin B1 and Aflatoxin M1 Alone and in Combination

Yang

Li²,

Gao

et al. 2023

Toxins

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“…37 AFB1 reportedly causes abnormal functional and morphological changes and oxidative stress in the duodenum. 38 However, LYC alleviated DON-induced oxidative stress by reducing ROS and MDA accumulation and enhancing the activity of antioxidant enzymes (SOD and GSH-Px) and T-AOC, which are linked to the activation of NRF2 signaling and a decline in KEAP1 expression. 39 Our results showed that co-exposure to ZEN, DON, and AFB1 caused oxidative damage (reduced T-AOC level and increased MDA and ROS contents) in the jejunum of mice.…”

Section: Papermentioning

confidence: 98%

Lycopene alleviates multiple-mycotoxin-induced toxicity by inhibiting mitochondrial damage and ferroptosis in the mouse jejunum

et al. 2022

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“…This beneficial effect is achieved through the inhibition of NF‐κB, iNOS, NOD1, and TLR2 pathways (Guo et al., 2021). Furthermore, the synergistic combination of L. casei , L. plantarum , Lactobacillus fermentum , and Lactobacillus delbrueckii in compound probiotics has been observed to ameliorate AFB 1 ‐induced disruptions in lipid metabolism in female albino rats (Ugbaja et al., 2020). Relative mRNA abundances of Bcl‐2, occludin, and ZO‐1 genes were significantly upregulated, while Bax, caspase‐3, GLUT2, ASCT2, PepT1, and IL‐6 genes were significantly downregulated upon the addition of compound probiotics ( B. subtilis , L. casein , C. utilis , and Aspergillus oryzae ) in IPEC‐J2 cells when compared to groups containing AFB 1 and/or ZEN (Huang et al., 2019).…”

Section: Effect Of Probiotics On Exposure To Foodborne Mycotoxinsmentioning

confidence: 99%

Insights into the intestinal toxicity of foodborne mycotoxins through gut microbiota: A comprehensive review

Ruan,

Huang,

Yue

et al. 2023

Comp Rev Food Sci Food Safe

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Mycotoxins, which are fungal metabolites, pose a significant global food safety concern by extensively contaminating food and feed, thereby seriously threatening public health and economic development. Many foodborne mycotoxins exhibit potent intestinal toxicity. However, the mechanisms underlying mycotoxin‐induced intestinal toxicity are diverse and complex, and effective prevention or treatment methods for this condition have not yet been established in clinical and animal husbandry practices. In recent years, there has been increasing attention to the role of gut microbiota in the occurrence and development of intestinal diseases. Hence, this review aims to provide a comprehensive summary of the intestinal toxicity mechanisms of six common foodborne mycotoxins. It also explores novel toxicity mechanisms through the “key gut microbiota–key metabolites–key targets” axis, utilizing multiomics and precision toxicology studies with a specific focus on gut microbiota. Additionally, we examine the potential beneficial effects of probiotic supplementation on mycotoxin‐induced toxicity based on initial gut microbiota‐mediated mycotoxicity. This review offers a systematic description of how mycotoxins impact gut microbiota, metabolites, and genes or proteins, providing valuable insights for subsequent toxicity studies of mycotoxins. Furthermore, it lays a theoretical foundation for preventing and treating intestinal toxicity caused by mycotoxins and advancing food safety practices.

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Probiotics consortium synergistically ameliorates aflatoxin B1-induced disruptions in lipid metabolism of female albino rats

Cited by 11 publications

References 42 publications

Integrated Metabolomics and Lipidomics Analysis Reveals Lipid Metabolic Disorder in NCM460 Cells Caused by Aflatoxin B1 and Aflatoxin M1 Alone and in Combination

Integrated Metabolomics and Lipidomics Analysis Reveals Lipid Metabolic Disorder in NCM460 Cells Caused by Aflatoxin B1 and Aflatoxin M1 Alone and in Combination

Lycopene alleviates multiple-mycotoxin-induced toxicity by inhibiting mitochondrial damage and ferroptosis in the mouse jejunum

Insights into the intestinal toxicity of foodborne mycotoxins through gut microbiota: A comprehensive review

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