Probiotics and Iletis

Jobin, Christian

doi:10.4161/gmic.1.3.12485

Cited by 15 publications

(7 citation statements)

References 52 publications

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“…Current medical treatments for IBD generally include 5-aminosalicylates or sulfasalazine, antibiotics, corticosteroids, immunomodulators and biological therapies (e.g., monoclonal antibodies). However, despite their efficacy, some patients are unresponsive to these therapies and often suffer from numerous side effects [40-42]. Thus, it is still necessary to develop new and specific therapies for IBD.…”

Section: Discussionmentioning

confidence: 99%

Plant flavonol isorhamnetin attenuates chemically induced inflammatory bowel disease via a PXR-dependent pathway

Dou

Zhang

et al. 2014

The Journal of Nutritional Biochemistry

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Isorhamnetin is an O-methylated flavonol present in fruit and vegetables. We recently reported the identification of isorhamnetin as an activator of the human pregnane X receptor (PXR), a known target for abrogating inflammation in inflammatory bowel disease (IBD). The current study investigated the role of isorhamnetin as a putative mouse PXR activator in ameliorating chemically induced IBD. Using two different models (Ulcerative colitis-like and Crohn’s disease-like) of experimental IBD in mice, we demonstrated that isorhamnetin abrogated inflammation through inhibiting the activity of myeloperoxidase (MPO), the levels of TNF-α and IL-6, the mRNA expression of pro-inflammatory mediators (iNOS, ICAM-1, COX2, TNF-α, IL-2 and IL-6), and the phosphorylation of IκBα and NF-κB p65. PXR gene overexpression inhibited NF-κB luciferase activity, and the inhibition was potentiated by isorhamnetin treatment. PXR knockdown by siRNA demonstrated the necessity for PXR in isorhamnetin-mediated upregulation of xenobiotic metabolism genes. Ligand pocket-filling mutants (S247W/C284W and S247W/C284W/S208W) of human PXR weakened the effect of isorhamnetin on PXR activation. Molecular docking studies and time-resolved fluorescence resonance energy transfer (TR-FRET) competitive binding assays confirmed the ligand (isorhamnetin) binding affinity. These results clearly demonstrated the ameliorating effect of isorhamnetin on experimental IBD via PXR-mediated upregulation of xenobiotic metabolism and downregulation of NF-κB signaling. The novel findings may contribute to the effective utilization of isorhamnetin or its derivatives as a PXR ligand in the treatment of human IBD.

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Section: Discussionmentioning

confidence: 99%

Plant flavonol isorhamnetin attenuates chemically induced inflammatory bowel disease via a PXR-dependent pathway

Dou

Zhang

et al. 2014

The Journal of Nutritional Biochemistry

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“…Many probiotic effects are mediated through immune regulation, particularly through balance control of proinflammatory and anti-inflammatory cytokines (Di Giacinto, Marinaro, Sanchez, Strober, & Boirivant, 1950;Foligne et al, 2010;Hacini-Rachinel et al, 1950;Jobin, 2010;Li, Xia, & Li, 2009). However, several animal and human studies have provided unequivocal evidence that specific strains of probiotics are able to stimulate several aspects of innate immunity (Amit-Romach et al, 2010;Boirivant et al, 2008;Boirivant & Strober, 2007;Farnell et al, 2006;Romanin et al, 2010;Weiss et al, 2010) as well as to increase humoral immunity (Fang, Elina, Heikki, & Seppo, 2000;Galdeano, de Leblanc Ade, Carmuega, Weill, & Perdigon, 2009;Leblanc, Fliss, & Matar, 2004;Nermes, Kantele, Atosuo, Salminen, & Isolauri, 2011).…”

Section: A Lactic Acid Bacteria-based Probiotic For Salmonella Contromentioning

confidence: 99%

Probiotics/direct fed microbials for Salmonella control in poultry

Téllez

Pixley

Wolfenden

et al. 2012

Food Research International

127

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“…Unfortunately, therapeutic options currently available for the treatment of IBD are largely limited to symptoms of the disease, with some exceptions to the molecular pathways associated with intestinal inflammation ( Jobin, 2010 ; Okafor et al, 2013 ). Despite advances in therapeutics, some patients didn’t respond to treatment or suffered from side effects or complications ( Dou et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Polysaccharides From Chrysanthemum morifolium Ramat Ameliorate Colitis Rats via Regulation of the Metabolic Profiling and NF-κ B/TLR4 and IL-6/JAK2/STAT3 Signaling Pathways

et al. 2018

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Studies have indicated that Chrysanthemum polysaccharides (CP) could prominently ameliorate colitis rats, but its possible mechanism remains unclear. In this study, the underlying mechanism of CP was explored by the metabolic profiling analysis and correlated signaling pathways. TNBS/ethanol induced colitis was used to investigate the intervention efficacy following oral administration of CP. The levels of cytokines such as TNF-α, IL-6, IFN-γ and IL-1β, and the activities of SOD, MPO, and MDA were determined. We also performed western-blot for p65, TLR4, p-JAK2, and STAT3 protein expression in the colon tissue to probe their mechanisms of correlated signaling pathways. What’s more, the metabolic changes in plasma and urine from colitis rats were investigated based on UPLC-Q-TOF/MS combined with MetabolynxTM software. The potential biomarkers and metabolic pathways were also tentatively confirmed. The metabolic profiles of plasma and urine were clearly improved in model rats after oral administration of CP. Thirty-two (17 in serum and 15 in urine) potential biomarkers were identified. The endogenous metabolites were mainly involved in linoleic acid, retinol, arachidonic acid, glycerophospholipid and primary bile acid metabolism in plasma, and nicotinate and nicotinamide, ascorbate and aldarate, histidine and β-alanine metabolism in urine. After polysaccharides intervention, these markers turned back to normal level at some extent. Meanwhile, the elevated expression levels of pp65, TLR4, p-STAT3, and p-JAK2 were significantly decreased after treatment. Results suggested that CP would be a potential prebiotics for alleviation of TNBS-induced colitis. The study paved the way for the further exploration of the pathogenesis, early diagnosis and curative drug development of the colitis.

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Probiotics and Iletis

Cited by 15 publications

References 52 publications

Plant flavonol isorhamnetin attenuates chemically induced inflammatory bowel disease via a PXR-dependent pathway

Plant flavonol isorhamnetin attenuates chemically induced inflammatory bowel disease via a PXR-dependent pathway

Probiotics/direct fed microbials for Salmonella control in poultry

Polysaccharides From Chrysanthemum morifolium Ramat Ameliorate Colitis Rats via Regulation of the Metabolic Profiling and NF-κ B/TLR4 and IL-6/JAK2/STAT3 Signaling Pathways

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