Probiotics and Blueberry Attenuate the Severity of Dextran Sulfate Sodium (DSS)-Induced Colitis

Abstract

We studied the anti-inflammatory properties of probiotic strains and blueberry in a colitis model. The disease activity index (DAI) was significantly lower on days 9 and 10 in all groups compared to the colitis control. Myeloperoxidase (MPO) and bacterial translocation to the liver and to the mesenteric lymph nodes (MLN) decreased significantly in all groups compared to colitis control. Cecal Enterobacteriaceae count decreased significantly in blueberry with and without probiotics compared to the other groups. Lactobacillus plantarum reisolated from the cecal content in the presence of blueberry, contrary to Lactobacillus fermentum. Colonic MDA decreased significantly in all groups, except the L. fermentum group, compared to the colitis control. The cecal concentration of acetic, propionic, and butyricbutyric acid was significantly higher in the L. plantarum group, while the L. fermentum group yielded the highest concentration of lactic acid compared with all other groups. Lactobacillus plantarum DSM 15313, Lactobacillus fermentum 35D, and blueberry alone and in combination improve the DAI, reduce bacterial translocation, and reduce inflammation.

Bilberry-Derived Anthocyanins Prevent IFN-γ-Induced Pro-Inflammatory Signalling and Cytokine Secretion in Human THP-1 Monocytic Cells

“…It has been previously demonstrated that anthocyanins from bilberries decrease pro-inflammatory cytokine serum levels and attenuate the severity of experimental colitis in mice [11,12,13]. Here, we have shown that BE clearly inhibits the pro-inflammatory effects of IFN-γ in human THP-1 monocytes as well as in human PBMCs.…”

“…A number of studies showed that polyphenols attenuate disease activity in IBD patients [11,12,13,14]. The aim of this cell culture model was to provide more information about the cellular mechanisms underlying the protective and anti-inflammatory properties of anthocyanins.…”

“…Various studies provide evidence that anthocyanin extracts inhibit inflammatory gene expression in vitro [8,9,10]. Moreover, it has been demonstrated that anthocyanins from bilberries (also called blueberries) decrease pro-inflammatory cytokine serum levels and attenuate the severity of experimental colitis in mice [11,12,13]. In an open-label pilot study in patients with mild to moderate ulcerative colitis, despite standard treatment, an anthocyanin-rich bilberry preparation significantly reduced disease activity, endoscopy scores and faecal calprotectin levels [14].…”

Effet de Lactobacillus LB inactivés et Milieu de Culture Fermenté sur la sévérité des symptômes : Observatoire sur 297 patients IBS diarrhéiques

“…Le rôle de la flore est de permettre la digestion des sucres grâce à la glycolyse dont les substrats finaux sont les acides gras volatils (acétate, butyrate et propionate). Ces acides représentent un apport énergétique et semblent capables d'interagir sur la micro inflammation [3]. La protéolyse dont certaines bactéries du colon sont capables apporte azote et carbone ; c'est la putréfaction.…”

The Effect of Inactivated <i>Lactobacillus</i> LB Fermented Culture Medium on Symptom Severity: Observational Investigation in 297 Patients with Diarrhea-Predominant Irritable Bowel Syndrome

“…The role of intestinal flora is the digestion of sugar through the process of glycolysis, the final substrates of which are volatile fatty acids (acetate, butyrate, and propionate). These acids provide energy and seem to have an effect on microinflammation [3]. The proteolytic activity of some bacteria in the colon results in the release of nitrogen and carbon, activating the process of putrefaction.…”