Probiotics administration or the high-fat diet arrest modulates microRNAs levels in hyperlipidemic hamsters

Niculescu, Loredan S.; Dulceanu, Madalina D.; Stancu, Camelia S.; Carnuta, Mihaela G.; Barbalata, Teodora; Sima, Anca V.

doi:10.1016/j.jff.2019.03.036

Cited by 7 publications

(5 citation statements)

References 24 publications

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“…We have previously demonstrated that miR-223 and miR-146a are specifically increased in plasma and HDL fractions from vulnerable CAD patients 18,19 . Also, we showed that miR-223 levels are augmented in plasma and livers from HFD-fed hamsters 17,21 . In other studies, we showed that hsa-miR-146a-5p and hsa-miR-21-5p are specifically associated with hyperlipidemia, being correlated with inflammatory parameters in HL patients 20 .…”

Section: Discussionmentioning

confidence: 68%

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Regulation of microRNAs in high-fat diet induced hyperlipidemic hamsters

Barbalata

Zhang

Dulceanu

et al. 2020

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Dyslipidemia is a documented risk factor for cardiovascular diseases and other metabolic disorders. Therefore, the analysis of hyperlipidemia (HL)-related miRNAs is a potential approach for achieving new prognostic markers in lipid-metabolism related diseases. We aimed to analyze specific distribution of miRNAs in different tissues from HL animals. Golden Syrian hamsters were fed either regular chow (NL) or high-fat diet (HL) for 12 weeks. Microarray miRNAs profiling was performed in liver, heart and small intestine and data analyzed by R-studio software. Functional enrichment bioinformatics analysis was performed using miRWalk and DAVID tools. We observed a dysregulation of miRNAs in HL tissues evidencing a discrete distribution in the heart-liver axis and three lipid metabolism-related miRNAs were identified: hsa-miR-223-3p, hsa-miR-21-5p, and hsa-miR-146a-5p. Expression levels of these miRNAs were increased in HL livers and hearts. Functional bioinformatics analysis showed involvement of these miRNAs in the regulation of biological processes altered in HL conditions such as lipid metabolic process, fat cell differentiation, regulation of smooth muscle cells and cardiac septum development. We identified a set of miRNAs dysregulated in different tissues of HFD-induced HL hamsters. These findings motivate further studies aiming to investigate novel molecular mechanisms of lipid metabolism and atherogenic HL.

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Section: Discussionmentioning

confidence: 68%

“…All the analyzed miRNAs are conserved between human and hamster (Crisetulus griseus). The tissue expression of hsa-miR-223-3p (ID 002295), hsa-miR-21-5p (ID 000397), and hsa-miR-146a-5p (ID 000468) were measured by using TaqMan technology (Thermo Sci., USA) as we previously reported, normalizing against snRNU6 (ID 001973) 17,21 .…”

Section: Methodsmentioning

confidence: 99%

Regulation of microRNAs in high-fat diet induced hyperlipidemic hamsters

Barbalata

Zhang

Dulceanu

et al. 2020

Sci Rep

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“…Published studies demonstrate that plasma miRNAs may be used as markers in the diagnosis of myocardial infarction [38], while EVs and HDL-associated miRNAs levels can discriminate between stable and vulnerable coronary artery disease patients [39][40][41]. It was reported that circulating and hepatic miRNAs expression could be modulated by hypolipidemic dietary interventions, such as probiotics administration [42]. For all these reasons, miRNAs could be very useful targets in nutritional science and could be used to test the pathways modulated by dietary treatments in healthy and/or diseased populations [43].Phytochemicals such as phenolic compounds have been extensively studied, and important data exist on their use in CVD patients.…”

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confidence: 99%

Phenolic Compounds Exerting Lipid-Regulatory, Anti-Inflammatory and Epigenetic Effects as Complementary Treatments in Cardiovascular Diseases

et al. 2020

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Atherosclerosis is the main process behind cardiovascular diseases (CVD), maladies which continue to be responsible for up to 70% of death worldwide. Despite the ongoing development of new and potent drugs, their incomplete efficacy, partial intolerance and numerous side effects make the search for new alternatives worthwhile. The focus of the scientific world turned to the potential of natural active compounds to prevent and treat CVD. Essential for effective prevention or treatment based on phytochemicals is to know their mechanisms of action according to their bioavailability and dosage. The present review is focused on the latest data about phenolic compounds and aims to collect and correlate the reliable existing knowledge concerning their molecular mechanisms of action to counteract important risk factors that contribute to the initiation and development of atherosclerosis: dyslipidemia, and oxidative and inflammatory-stress. The selection of phenolic compounds was made to prove their multiple benefic effects and endorse them as CVD remedies, complementary to allopathic drugs. The review also highlights some aspects that still need clear scientific explanations and draws up some new molecular approaches to validate phenolic compounds for CVD complementary therapy in the near future.In the last decade the scientific researchers turned their attention to phytochemicals, as effective, safe and low-cost natural bioactive compounds for CVD treatment.Dyslipidemia consists of increased blood concentrations of total cholesterol (TC), low density lipoproteins-cholesterol (LDL-C) and/or triglycerides (TG), and decreased high density lipoproteins-cholesterol (HDL-C) [6]. The lipid metabolism is complex and the candidate mechanisms that could generate dyslipidemia include: (i) excessive dietary lipid absorption in the small intestine; (ii) packing of exogenous lipids with cholesterol and fatty acids produced de novo in the liver and their secretion as very low density lipoproteins (VLDL); (iii) hydrolysis of TG from VLDL by lipases and their conversion into LDL, which are taken up by the peripheral tissues through LDL receptor (LDL-R) and scavenger receptors; (iv) diminished production of HDL by the liver and small intestine, thereby decreasing reverse cholesterol transport (RCT) from the peripheral tissues to the liver; (v) lowered excess cholesterol excretion from the liver into gallbladder or to the intestinal lumen through the ATP-binding cassette G5 and G8 transporters (ABCG5/G8) that facilitate trans-intestinal cholesterol efflux (TICE). Dyslipidemia is associated with the accumulation of LDL in the sub-endothelium of the artery wall. At this site, LDL undergoes oxidative modifications (oxLDL) that trigger inflammatory responses, and is taken up by the monocyte-derived macrophages infiltrated in the sub-endothelium which thus become lipid-loaded foam cells, the hallmark of atheroma development [7]. Until now, the most effective lipid-lowering treatment for hyperlipidemic patients was the statin therapy. But recen...

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“…However, the importance of dietary interventions in improving hyperlipidemia is still emphasized in addition to drug therapy. Valuable findings suggest that some dietary supplements (such as probiotics, functional foods) can improve hyperlipidemiar ( 9 , 16 , 24 ). Our results also confirmed the feasibility of probiotic-fermented foods for improving dyslipidemia induced by HFD.…”

Section: Discussionmentioning

confidence: 99%

“…In addition to functional cereals, the anti-hyperlipidemia efficacies of probiotics have been widely studied. Several studies indicated that probiotics might induce anti-hyperlipidemia effects by promoting fatty acid oxidation, reducing lipid-related gene expression, and regulating intestinal microbiota ( 15 , 16 ). Bifidobacterium longum has been shown to reduce serum TG, TC, and LDL-C levels and enhance the activity of superoxide dismutase (SOD) in the liver of HFD-fed mice ( 17 ).…”

Section: Introductionmentioning

confidence: 99%

Coix Seed-Based Milk Fermented With Limosilactobacillus reuteri Improves Lipid Metabolism and Gut Microbiota in Mice Fed With a High-Fat Diet

et al. 2022

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The aim of this study was to investigate the effects of coix seed-based milk (CSM) fermented with Limosilactobacillus reuteri (L. reuteri) on dyslipidemia and the composition of the intestinal microbiota in high fat diet (HFD)-fed mice. Changes in the body weight, serum lipid levels, activities of hepatic oxidative stress factors, expression of lipid-related genes, and composition of the intestinal microbiota of HFD-fed mice after supplementation with CSM were determined. The results showed that intake of CSM reduced the body weight gain as well as serum total cholesterol (TC), triglyceride (TG), and low-density lipoprotein cholesterol (LDL-C) levels, and increased the high-density lipoprotein cholesterol (HDL-C) levels in the mice. Meanwhile, supplementation with CSM could relieve liver oxidative stress, down-regulate the expression of genes related to lipid synthesis, and prevent liver fat accumulation in mice fed with HFD. The 16S rRNA sequencing of the intestinal microbiota showed that CSM regulated the gut microbiota community structure at different taxonomic levels, and reversed gut dysbiosis induced by HFD. The relative abundance of Muribaculaceae, Lachnospiraceae, Dubosiella and Akkermansia which are negatively correlated with blood lipid levels were significantly increased by the intervention of CSM, while the relative abundance of Desulfovibrionaceae, Ruminococca-ceae_UCG-014, Psychrobacter, and Staphylococcus which have positive correlation with blood lipid levels were significantly decreased. These results indicated that CSM might serve as a novel and promising dietary supplement for ameliorating hyperlipidemia and intestinal microbiota disorders caused by HFDs.

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Probiotics administration or the high-fat diet arrest modulates microRNAs levels in hyperlipidemic hamsters

Cited by 7 publications

References 24 publications

Regulation of microRNAs in high-fat diet induced hyperlipidemic hamsters

Regulation of microRNAs in high-fat diet induced hyperlipidemic hamsters

Phenolic Compounds Exerting Lipid-Regulatory, Anti-Inflammatory and Epigenetic Effects as Complementary Treatments in Cardiovascular Diseases

Coix Seed-Based Milk Fermented With Limosilactobacillus reuteri Improves Lipid Metabolism and Gut Microbiota in Mice Fed With a High-Fat Diet

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