Probiotic Yogurt Alleviates High-Fat Diet-Induced Lipid Accumulation and Insulin Resistance in Mice via the Adiponectin Pathway

Abstract

A high-fat diet (HFD) easily contributes to the pathogenesis of obesity and insulin resistance. Obesity and insulin resistance have been clinical and public health challenges all over the world. Probiotic-fermented yogurt is one type of popular and functional beverage in people’s daily lives. This study mainly explored the lipid- and glucose-lowering effects of Lactobacillus acidophilus NX2-6-fermented yogurt (LA-Y) in HFD-fed mice. The results showed that LA-Y administration improved the lipid profile in the serum and liver, reduced fasting blood glucose levels, and enhanced insulin sensitivity. Protein analysis showed that LA-Y treatment promoted fatty acid oxidation and suppressed de novo lipogenesis in the adipose tissue and liver. LA-Y effectively alleviated glucose metabolism disorders by activating the insulin signaling pathway, suppressing gluconeogenesis in the liver and muscle, reducing the concentration of pro-inflammatory cytokines in the serum, and promoting glycolysis and gluconeogenesis in the small intestine. LA-Y supplementation also promoted fat browning via the adiponectin/AMPKα/PGC-1α/UCP1 pathway and enhanced mitochondrial biogenesis in the liver and muscle by activating the adiponectin/AdipoR1/APPL1/AMPKα/PGC-1α pathway, leading to increased energy expenditure. Therefore, LA-Y may be a functional dairy food for preventing and alleviating diet-induced metabolic disorders.

Ingestion of Lacticaseibacillus rhamnosus Fmb14 prevents depression-like behavior and brain neural activity via the microbiota–gut–brain axis in colitis mice

“…Western blotting was performed as described by Tang. 81 The colon and prefrontal cortex of brain tissues (500 mg) were placed in a mortar along with liquid nitrogen and promptly crushed into powder. The powder was added to 500 μL of RIPA lysis buffer (10 μL mg −1 ) (Beyotime Biotech, Shanghai, China) containing PMSF and lysed on ice for 30 min after vortexing.…”

Mechanisms of probiotic modulation of ovarian sex hormone production and metabolism: a review

“…182 Currently, yogurt fermented with Lactobacillus acidophilus NX2-6 has been demonstrated to lessen lipid deposition and insulin resistance in mice via the adiponectin pathway. 183 The alleviation of insulin resistance itself reduces androgen secretion. Adiponectin in the ovary is primarily produced by the surrounding fat pad, which significantly influences the production of sex steroid hormones in ovarian granulosa cells.…”

Lacticaseibacillus rhamnosus HF01 fermented yogurt alleviated high-fat diet-induced obesity and hepatic steatosis via the gut microbiota–butyric acid–hepatic lipid metabolism axis

“…Many studies have demonstrated the potential benefits of probiotic fermented yogurt, including its hypotensive, hypocholesterolemic, anti-diabetic, and anti-obesity properties, as well as its ability to reduce non-alcoholic fatty liver and improve intestinal health. [17][18][19] The research shows that the intake of yogurt fermented by Levilactobacillus brevis FZU0713 has changed the composition of the gut microbiota and increased the level of SCFAs. 20 Lacticaseibacillus rhamnosus fermented yogurt can enhance the nutritional value of yogurt, regulate the gut microbiota, and improve gut health.…”