Probiotic supplements containing Lactobacillus reuteri does not affect the levels of matrix metalloproteinases and interferons in oral wound healing

Abstract

ObjectiveThe use of beneficial bacteria may stimulate wound healing. We performed a randomized, placebo-controlled double-blind cross-over study comprising ten healthy volunteers. The aim was to investigate the impact of topical and systemic applications of probiotic lactobacilli (Lactobacillus reuteri) on the healing of standardized wounds (punch biopsies) in the oral mucosa. The expression of selected matrix metalloproteinases (MMP’S) and interferons (IFN’s) was analyzed with multiplex immunoassays in the wound exudate during the first healing week (day 2, 5 and 8).ResultsAll participants completed the study and in all cases, the healing after the punch biopsies was uneventful. The concentrations of MMP-1, MMP-2, MMP-3 decreased with time in both the test- and control group. The MMP levels were consistently lower during the probiotic intervention when compared with placebo but the differences were not statistically significant. Likewise, the concentrations if IFN-alpha2, IFN-beta and IFN-gamma decreased with time with no significant differences between the test and placebo interventions. Within the limitations of this pilot study, we were unable to demonstrate an influence of probiotic supplements containing L. reuteri on the concentrations of selected matrix metalloproteinases and interferons from mucosal wounds within 1 week after a standardized punch biopsy.Trial registration ClinicalTrials.gov Identifier NCT03210779. Date of registration: July 7, 2017Electronic supplementary materialThe online version of this article (10.1186/s13104-018-3873-9) contains supplementary material, which is available to authorized users.

The role of topical probiotics on wound healing: A review of animal and human studies

“…In these studies, the mechanism of action was typically not explored, but topical probiotic treatment resulted in improved healing as demonstrated by increased granulation tissue deposition, improved collagen concentration, and stimulation of angiogenesis. [33][34][35][36][37][38][39][40][41][42] However, not all models demonstrated an improvement in topical wound healing with probiotics [33][34][35][36][37][38][39][40][41][42][43][44] (Tables 1 and 2).…”

“…They were able to reduce the erythema and oedema associated with CO 2 laser therapy, reduce skin sensitivity in patients with reactive skin, and increase ceramide concentration and skin hydration. [82][83][84][85] However, not all studies investigating topical probiotics have demonstrated superiority compared with traditional interventions 43,44 (Table 2).…”

Investigating the anti‐inflammatory effects of high molecular weight secretions from Limosilactobacillus reuteri PTCC 1655 on LPS‐stimulated PMA‐differentiated THP‐1 cells

“…Activities of MMPs are regulated by TIMPs (Dollery et al 1995;Nagase et al 2006). Twetman et al (2018) suggested that enrichments encompassing L. reuteri cannot influence the levels of MMP and interferons in oral wound healing. Our results of gelatin zymography and RT-qPCR revealed that the activity and expression of MMP9 significantly decreased by HK-SON, unprocessed CSF and >100 kDa fraction of L. reuteri PTCC 1655 in comparison with the related controls.…”

The role of topical probiotics in skin conditions: A systematic review of animal and human studies and implications for future therapies

“…Numerous human studies have investigated the role of topical probiotics in various skin conditions . Skin conditions that have been investigated include atopic dermatitis, seborrhoeic dermatitis, acne and reactive skin.…”