Probiotic properties of Oxalobacter formigenes: an in vitro examination

Ellis, Melissa L.; Dowell, Alexander E.; Li, Xingsheng; Knight, John

doi:10.1007/s00203-016-1272-y

Cited by 20 publications

(13 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is known that several anaerobic bacteria show oxygen tolerance and possess systems to respond to oxygen (46)(47)(48). In this study, we demonstrated that O. formigenes survives in fecal pellets excreted from animals monocolonized with O. formigenes, which is consistent with our recent finding that O. formigenes survives in static broth cultures exposed to the air (49). Of note, the genomes of two O. formigenes strains (50) and the proteome of O. formigenes OxCC13 (51) contain superoxide dismutase, which may play a key role in the survival of O. formigenes during periods of oxygen exposure.…”

Section: Discussionsupporting

confidence: 92%

“…Interestingly, group-housed GF/OxF animals had small numbers of viable O. formigenes organisms throughout the gastrointestinal tract. This finding not only highlights the coprophagic behavior of group-housed mice in regular cages but also supports previous in vitro reports showing that O. formigenes tolerates exposure to acid and bile (3,49).…”

Section: Discussionsupporting

confidence: 90%

See 1 more Smart Citation

Response of Germfree Mice to Colonization by Oxalobacter formigenes and Altered Schaedler Flora

Ellis

Dowell

et al. 2016

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

Colonization with Oxalobacter formigenes may reduce the risk of calcium oxalate kidney stone disease. To improve our limited understanding of host-O. formigenes and microbe-O. formigenes interactions, germfree mice and mice with altered Schaedler flora (ASF) were colonized with O. formigenes. Germfree mice were stably colonized with O. formigenes, which suggests that O. formigenes does not require other organisms to sustain its survival. Examination of intestinal material indicated no viable O. formigenes in the small intestine and ϳ4 ؋ 10 6 CFU O. formigenes per 100 mg contents in the cecum and proximal colon, with ϳ0.02% of total cecal O. formigenes cells being tightly associated with the mucosa. O. formigenes did not alter the overall microbial composition of ASF, and ASF did not affect the capacity of O. formigenes to degrade dietary oxalate in the cecum. Twentyfour-hour collections of urine and feces in metabolic cages in semirigid isolators demonstrated that the introduction of ASF into germfree mice significantly reduced urinary oxalate excretion. These experiments also showed that O. formigenes-monocolonized mice excreted significantly more urinary calcium than did germfree mice, which may be due to degradation of calcium oxalate crystals by O. formigenes and subsequent intestinal absorption of free calcium. In conclusion, the successful establishment of mouse models with defined flora and O. formigenes should improve our understanding of O. formigenes-host and O. formigenes-microbe interactions. These data support the use of O. formigenes as a probiotic that has limited impact on the composition of the resident microbiota but provides an efficient oxalate-degrading function. Oxalobacter formigenes is part of the bacterial flora in the large intestine of many humans and other mammalian species and is unique in that it is the only bacterium yet identified in the intestines of mammals that requires oxalate as both an energy source and a carbon source. Recent evidence suggests that a lack of colonization with this oxalate-degrading specialist is a risk factor for idiopathic recurrent calcium oxalate stone disease (1, 2). A review of worldwide data indicated that 38% to 77% of a normal population and only 17% of stone formers were colonized with O. formigenes (1). The ability to recolonize individuals lacking O. formigenes was previously addressed in a study in which two healthy adults not colonized with O. formigenes became colonized following the ingestion of cultured O. formigenes (3) and remained colonized for 9 months. However, studies in which O. formigenes was provided in either a lyophilized form in enteric coated capsules or as a frozen paste to patients suffering from primary hyperoxaluria resulted in only a minority of the patients remaining colonized posttreatment (4, 5). Therefore, although it seems quite possible that O. formigenes colonization of noncolonized stone formers may be an effective way to minimize the risk of recurrent calcium oxalate stone disease, a better understanding o...

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 90%

Response of Germfree Mice to Colonization by Oxalobacter formigenes and Altered Schaedler Flora

Ellis

Dowell

et al. 2016

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Among them, oxalate occupies the vast majority, exceeding 85%. The microbiota refers to the entire microbial population that colonizes in a specific location and includes not only bacteria but also other microorganisms such as fungi, archaea, viruses, and protozoa [ 8 ]. The scientific community has shown great interest in the gut microbiota in recent years; the gut microbiota is associated with many human diseases, such as intestinal diseases such as inflammatory bowel disease (IBD) [ 9 ] and irritable bowel syndrome (IBS) [ 10 ], metabolic diseases such as obesity and diabetes [ 11 ], and allergic diseases [ 12 ] to neurodevelopmental diseases, although the strength of some evidence is not sound in many of them.…”

Section: Discussionmentioning

confidence: 99%

[Retracted] The Relationship between Urinary Stones and Gut Microbiomeby 16S Sequencing

Zhou

Zhao

et al. 2020

BioMed Research International

View full text Add to dashboard Cite

Objective. To understand the relationship between urinary stones and the gut microbiome and to screen for microbial species that may be involved in stone formation. Methods. Stool samples were collected from patients with urolithiasis and healthy patients between March and December 2017. The samples were analyzed by 16S sequencing to determine differences in the microbiome profiles between the two groups. The mouse model was established and was divided into two groups. Fecal samples were collected from the mice before gavage and three weeks postgavage for microbiome analysis. The microbial population of each group was analyzed to screen for microbial species that may affect the formation of urinary stones. Differences in the number of crystals in the renal tubules of the mice were examined by necropsy. Results. The microbial composition was different between urolithiasis patients and healthy controls. The urolithiasis patients had significantly reduced microbial abundance; however, increased proportions of Bacteroidetes and Actinobacteria were detected compared to healthy controls. Furthermore, the abundance of Alistipesindistinctus and Odoribactersplanchnicus was significantly increased in the urolithiasis patients compared to the healthy controls. In addition, the incidence of urolithiasis was much higher in the experimental mouse group (stone solution + urolithiasis patient stool) than in the control mouse group. However, the microbial abundance before gavage was not significantly different from that seen three weeks postgavage. Conclusion. Theurolithiasis patients in this study had a different gut microbiome when compared with that of healthy individuals. The altered microbiome increased the rate of crystal formation in renal tubules and accelerated urinary stone formation in the mouse model of urolithiasis.

show abstract

“…The genus Adlercreutzia only has one species, namely, A. equolifaciens , which can produce equol [ 60 ]. The genus Oxalobacter commonly inhabits the intestine and can degrade oxalate as its major energy [ 61 ] (Liu et al, 2016). The desulfitobacter spp.…”

Section: Discussionmentioning

confidence: 99%

Zearalenone Changes the Diversity and Composition of Caecum Microbiota in Weaned Rabbit

Yang

Zhang

Huang

et al. 2018

BioMed Research International

View full text Add to dashboard Cite

Mycotoxins exhibit several severe effects on intestinal health, but few studies have assessed mycotoxins effect on the intestinal microflora and its repercussions to humans and animals. In this study, we evaluated the effect of zearalenone (ZEA), one of the most harmful mycotoxins on the structure of caecal microbiota in rabbits. Twenty-eight male weaned rabbits were randomly divided into four groups and orally given different concentrations of ZEA (400, 800, and 1600 μg/kg.b.w). Microbial communities in caecum samples of rabbits were analyzed for 16S rRNA by Illumina sequencing through Illumina Miseq platform after being fed for 28 days. The results showed that increasing ZEA doses increased the species richness but did not significantly increased the species diversity of the caecum microbiota in the rabbits. In addition, the caecum microbiota from the samples in different ZEA-treated groups was clustered according to their dosing regimens. At the phylum level, ZEA decreased the abundance of Actinobacteria and significantly increased the abundance of Cyanobacteria, Synergistetes, and Proteobacteria. At the genus level, there were declines in the abundance of Adlercreutzia, Blautia, Desulfitobacter, Lactobacillus, Oxalobacter, and p-75-a5. The decrease of abundance in Lactobacillus, Desulfitobacter, and p-75-a5 was particularly noticeable. In conclusion, zearalenone could increase α-diversity but significantly decrease the abundance of some bacteria with the important metabolic functions. These findings suggested that ZEA could modify the caecum microbiota.

show abstract

Probiotic properties of Oxalobacter formigenes: an in vitro examination

Cited by 20 publications

References 36 publications

Response of Germfree Mice to Colonization by Oxalobacter formigenes and Altered Schaedler Flora

Response of Germfree Mice to Colonization by Oxalobacter formigenes and Altered Schaedler Flora

[Retracted] The Relationship between Urinary Stones and Gut Microbiomeby 16S Sequencing

Zearalenone Changes the Diversity and Composition of Caecum Microbiota in Weaned Rabbit

Contact Info

Product

Resources

About