Probiotic properties of native Lactobacillus spp. strains for dairy calves

Abstract

The use of native microorganisms with probiotic capacity is an alternative tool for the treatment and prevention of several diseases that affect animals, such as neonatal calf diarrhoea. The selection of probiotic strains within a collection is based on different in vitro and in vivo assays, which predict their potential. The aim of this study was to characterise a group of native Lactobacillus spp. strains isolated from faeces of healthy calves using an in vitro approach and to assess their ability to colonise the gastrointestinal tract (GIT) of calves. Native Lactobacillus spp. strains were evaluated on their capacity to survive low pH conditions and bile salts presence, biofilm formation and adhesion to both mucus and Caco-2 cells. Based on the in vitro characterisation, four strains (Lactobacillus johnsonii TP1.1, Lactobacillus reuteri TP1.3B, L. johnsonii TP1.6 and Lactobacillus amylovorus TP8.7) were selected to evaluate their capacity to colonise and persist in the GIT of calves. The assessment of enteric persistence involved an in vivo assay with oral administration of probiotics and quantification in faeces of the administered bacterial species with real-time quantitative PCR (qPCR). The study was conducted using 15 calves (1-month-old) which were divided into five groups of three animals, four of which were treated with four different selected strains and one was the control group. Strains TP1.3B and TP1.6 managed to persist in treated animals until ten days after the end of the administration period, indicating that they could be promising candidates for the design of probiotics for calves.

The safety and potential probiotic properties analysis of Streptococcus alactolyticus strain FGM isolated from the chicken cecum

“…Meanwhile, competition from lactic acid bacteria for nutrition also limits the excessive multiplication of pathogenic bacteria and maintains the balance of intestinal microecology (Tallon et al 2007). Lactobacillus known as probiotics showed strong adherence and inhibition to pathogens adhesion and growth (Ma et al 2006;Fernández et al 2018). In this paper, we compared S. alactolyticus strain FGM with three lactobacillus strains on pathogens inhibition.…”

Metagenomics analysis revealed the distinctive ruminal microbiome and resistive profiles in dairy buffaloes

“…It has been found that L. amylovorus and L. acidophilus exert beneficial effects on gastrointestinal immunomodulation and health as candidate bacterial therapeutics, such as mitigating the bovine respiratory pathogens in dairy calves [ 67 ]. The probiotic properties of L. amylovorus and L. acidophilus have been well studied [ 68 , 69 ], suggesting the promising cholesterol-lowering properties [ 70 ], anti-pathogens effect [ 71 ], and prevention of disease in human and dairy calves [ 72 ]. The significantly higher relative abundance of these two species especially for L. amylovorus (2% in young dairy buffaloes) in the rumen of dairy buffaloes may provide probiotic resource for fermented feed.…”

Differences in the fecal microbiota of dairy calves reared with differing sources of milk and levels of maternal contact

“…Lactobacillus spp. have been shown to have probiotic effects for calves and offer protection against calf scours (Fernández et al, 2018). According to Malmuthuge et al (2019), early exposure to Lactobacillus may prime the host (via initial expression of key proinflammatory chemokines) for future anti-inflammatory responses.…”