Probiotic Properties and Proteomic Analysis of Pediococcus pentosaceus 1101

Abstract

Pediococcus pentosaceus 1101 was identified by using 16S rRNA and MALDI-Biotyper. The strain was exposed to conditions that resemble the gastrointestinal tract (GT) to evaluate its probiotic properties. That included the growth kinetics, proteolytic and inhibitory activities within a pH range, survival at low pH and in the presence of bile salts, antagonistic activity, cell-adhesion properties, and antibiotic resistance. The evaluation was followed by a genomic and proteomic analysis that involved the identification of proteins obtained under control and gastrointestinal conditions. The strain showed antagonistic activity against Gram-negative and Gram-positive bacteria, high resistance to acidity (87% logarithmic survival rate, pH 2) and bile salts (99% logarithmic survival rate, 0.5% w/v), and hydrophobic binding, as well as sensitivity to penicillin, amoxicillin, and chloramphenicol. On the other hand, P. pentosaceus 1101 has a genome size of 1.76 Mbp, with 1754 coding sequences, 55 rRNAs, and 33 tRNAs. The proteomic analysis showed that 120 proteins were involved in mechanisms in which the strain senses the effects of acid and bile salts. Moreover, the strain produces at least one lytic enzyme (N-acetylmuramoyl-L-alanine amidase; 32 kDa) that may be related to the antimicrobial activity. Therefore, proteins identified might be a key factor when it comes to the adaptation of P. pentosaceus 1101 into the GT and associated with its technological and probiotic properties.

Evaluation of Safety and Probiotic Traits from a Comprehensive Genome-Based In Silico Analysis of Ligilactobacillus salivarius P1CEA3, Isolated from Pigs and Producer of Nisin S

“…Genes encoding proteins related to tolerance to temperature, acid, pH, bile salts, osmotic and oxidative stress were also identified in the L. salivarius P1CEA3 genome ( Table S4 ), suggesting a high level of stress adaptation and bacterial survival [ 91 , 92 ]. Genes encoding the molecular co-chaperones GroES and GroEL, involved in the refolding of many proteins, also supports the probiotic potential of L. salivarius P1CEA3 to minimize the impact of the high temperatures reached during the processing of animal feed [ 93 ].…”

Use of Lactulose as Prebiotic and Chitosan Coating for Improvement the Viability of Lactobacillus sp. FM4.C1.2 Microencapsulate with Alginate

“…The free probiotic bacteria (T0) exhibited tolerance to bile salts, maintaining viability above 80% during the 3 h of exposure. This behavior reveals that the strain FM4C1.2 evaluated here has high activity of the bile salt hydrolase (BSH) enzyme, responsible for deconjugating bile acid, thus reducing its toxic effect [48] and, also may contribute to the metabolism of fatty acids and cholesterol. The microencapsulation and coating processes improved the viability of the strain.…”

Acetolactate Decarboxylase as an Important Regulator of Intracellular Acidification, Morphological Features, and Antagonism Properties in the Probiotic Lactobacillus reuteri

“…Tolerance to SGF and low pH, which are representative of the acid condition of the stomach, is a fundamental characteristic of probiotic selection. [ 65 ] The extreme environment in the stomach is an important barrier. Overcoming this deficiency is necessary for the development of colonies of probiotic bacteria following in vitro administration.…”