Probiotic properties and immunomodulatory activity of gastrointestinal tract commensal bacterial strains isolated from the edible farmed snail Cornu aspersum maxima

Dushku, Esmeralda; Ioannou, Athanasia; Στάικου, Αλεξάνδρα; Yiangou, Minas

doi:10.1016/j.fsi.2019.06.061

Cited by 15 publications

(9 citation statements)

References 44 publications

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“…One of the most important criteria for selecting LAB with probiotic properties is the ability to tolerate the environment of the gastrointestinal tract, with the pH of pig gastric juices being as low as 2.0, and bile with a pH of about 8.0. Dushku et al [ 14 ] isolated and identified 40 LAB strains from the gastrointestinal tract of local snails, which were artificially simulated to survive the gastrointestinal tract of only two strains. Rao et al [ 15 ] screened L. plantarum AT4 for low pH and up to 0.5% bile salts.…”

Section: Discussionmentioning

confidence: 99%

Screening of Lactobacillus plantarum subsp. plantarum with Potential Probiotic Activities for Inhibiting ETEC K88 in Weaned Piglets

Wang

et al. 2020

Molecules

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For screening excellent lactic acid bacteria (LAB) strains to inhibit enterotoxigenic Escherichia coli (ETEC) K88, inhibitory activities of more than 1100 LAB strains isolated from different materials, and kept in the lab, were evaluated in this study. Nine strains with inhibition zones, at least 22.00 mm (including that of a hole puncher, 10.00 mm), and good physiological and biochemical characteristics identified by 16S DNA gene sequencing and recA gene multiple detection, were assigned to Lactobacillus (L.) plantarum subsp. plantarum (5), L. fermentum (1), L. reuteri (1), Weissella cibaria (1) and Enterococcus faecalis (1), respectively. As investigated for their tolerance abilities and safety, only strain ZA3 possessed high hydrophobicity and auto-aggregation abilities, had high survival rate in low pH, bile salt environment, and gastrointestinal (GI) fluids, was sensitive to ampicillin, and resistant to norfloxacin and amikacin, without hemolytic activity, and did not carry antibiotic resistance genes, but exhibited broad spectrum activity against a wide range of microorganisms. Antibacterial substance may attribute to organic acids, especially lactic acid and acetic acid. The results indicated that the selected strain L. plantarum subsp. plantarum ZA3 could be considered a potential probiotic to inhibit ETEC K88 in weaned piglets for further research.

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Section: Discussionmentioning

confidence: 99%

Screening of Lactobacillus plantarum subsp. plantarum with Potential Probiotic Activities for Inhibiting ETEC K88 in Weaned Piglets

Wang

et al. 2020

Molecules

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“…Gut microbes not only provide various key digestive enzymes for the snail host (Brendelberger, 1997;Ito et al, 2019) but also strongly affect many physiological processes, such as uric acid oxidation (Van Horn et al, 2012), cold hardiness (Nicolai et al, 2015), and amino acid and energy metabolism (Grandiosa et al, 2018), which were once thought to depend only on the gastropod host genome. Some gut microbes can even adhere to intestinal mucosa and stimulate immune response that is mediated by the regulation of the expression of Toll-like receptors in snail intestinal mucosa (Dushku et al, 2019). Moreover, unlike the largely static host genome, the symbiotic microbiome is highly plastic and can rapidly respond to changes in host diet or environmental conditions through changes in community composition, mutations, exchange of genetic material with bacteria from the environment, or changes in gene expression (Shapira, 2016), as well as enhance host adaptation to the fast and drastic environmental changes by influencing various physiological activities of the host (Gilbert et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Responses of Gut Microbial Community Composition and Function of the Freshwater Gastropod Bellamya aeruginosa to Cyanobacterial Bloom

et al. 2022

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Freshwater gastropods are widely distributed and play an important role in aquatic ecosystems. Symbiotic microorganisms represented by gut microbes can affect the physiological and biochemical activities of their hosts. However, few studies have investigated the response of the gut microbial community of snails to environmental stress. In this study, the dynamics of the gut microbiota of the gastropod Bellamya aeruginosa were tracked to explore their responses in terms of their composition and function to cyanobacterial bloom. Differences in gut microbial community structures during periods of non-cyanobacterial bloom and cyanobacterial bloom were determined. Results showed that the alpha diversity of the gut microbiota exposed to cyanobacterial bloom was lower than that of the gut microbiota exposed to non-cyanobacterial bloom. The main genera differentiating the two periods were Faecalibacterium, Subdoligranulum, Ralstonia, and Pelomonas. Microcystins (MCs) and water temperature (WT) were the primary factors influencing the gut microbial community of B. aeruginosa; between them, the influence of MCs was greater than that of WT. Fourteen pathways (level 2) were notably different between the two periods. The pathways of carbohydrate metabolism, immune system, environmental adaptation, and xenobiotics biodegradation and metabolism in these differential pathways exhibited a strong linear regression relationship with MCs and WT. Changes in the functions of the gut microbiota may help B. aeruginosa meet its immunity and energy needs during cyanobacterial bloom stress. These results provide key information for understanding the response pattern of freshwater snail intestinal flora to cyanobacterial blooms and reveal the underlying environmental adaptation mechanism of gastropods from the perspective of intestinal flora.

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“…One of the most important criteria for selecting LAB with probiotic properties is the ability to tolerate the environment of the gastrointestinal tract, with the pH of pig gastric juices being as low as 2.0 and bile with a pH of about 8.0. Dushku et al [10] isolated and identified 40 LAB strains from the gastrointestinal tract of local snails, which were artificially simulated to survive the gastrointestinal tract only 2 strains; Rao et al [11] screened L. plantarum AT4 for low pH and up to 0.5% bile salts. This study found 40 selected strains with excellent inhibition abilities, 13 homo-fermentative isolates could grow at 5-50°C and pH 3.0-10.0, and ferment glucose without producing gas.…”

Section: Treatmentmentioning

confidence: 99%

Screening of Lactobacillus plantarum subsp. plantarum with Potential Probiotic Activities for Inhibiting ETEC K88

Wang¹,

Ma²,

Yu³

et al. 2020

Preprint

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For screening excellent lactic acid bacteria (LAB) strains to inhibit Escherichia (E.) coli (ETEC) K88, inhibitory activities of more than 1100 LAB strains isolated from different materials and kept in the lab were evaluated in this study. Nine strains with inhibition zone at least 22.00 mm (including that of hole puncher 10.00 mm) and good physiological and biochemical characteristics identified by 16S DNA gene sequencing and recA gene multiple detection, were assigned to Lactobacillus (L.) plantarum subsp. plantarum (5), L. fermentum (1), L. reuteri (1), W. cibaria (1) and E. faecalis (1), respectively. As investigated for their tolerance abilities and safety, only strain ZA3 possessed high hydrophobicity and auto-aggregation abilities, had high survival rate in low pH, bile salt environment and GI fluids, sensitive to ampicillin, resistant to norfloxacin and amikacin, without hemolytic activity and didn’t carry antibiotic resistance genes, exhibited broad spectrum activity against a wide range of microorganisms, and antibacterial substance may attribute to organic acids, especially lactic acid and acetic acid. The results indicated that the selected strain L. plantarum subsp. plantarum ZA3 could be considered a potential probiotic to inhibit ETEC K88 for further research.

show abstract

Probiotic properties and immunomodulatory activity of gastrointestinal tract commensal bacterial strains isolated from the edible farmed snail Cornu aspersum maxima

Cited by 15 publications

References 44 publications

Screening of Lactobacillus plantarum subsp. plantarum with Potential Probiotic Activities for Inhibiting ETEC K88 in Weaned Piglets

Screening of Lactobacillus plantarum subsp. plantarum with Potential Probiotic Activities for Inhibiting ETEC K88 in Weaned Piglets

Responses of Gut Microbial Community Composition and Function of the Freshwater Gastropod Bellamya aeruginosa to Cyanobacterial Bloom

Screening of <em>Lactobacillus</em> <em>plantarum</em> subsp. <em>plantarum</em> with Potential Probiotic Activities for Inhibiting ETEC K88

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