Probiotic Potential of Lactic Acid Starter Cultures Isolated from a Traditional Fermented Sorghum-Millet Beverage

Abstract

The purpose of this study was to establish the probiotic potential of lactic acid bacteria (LAB) starter cultures, Lb. plantarum MNC 21, L. lactis MNC 24, and W. confusa MNC 20, isolated from a traditionally fermented sorghum-millet beverage from Uganda. The cultures were examined for tolerance to acid and bile salts, bile salt hydrolase (BSH) activity, antibiotic susceptibility, biogenic amine production, mucin degradation, hydrophobicity, auto-aggregation, adherence to the ileum, coaggregation, and antimicrobial properties against selected pathogenic species. Lb. rhamnosus yoba 2012, a known probiotic, was the reference. The isolates were tolerant to acid (pH = 3) and bile (1%). W. confusa MNC 20 and Lb. plantarum MNC 21 exhibited medium BSH activity (11–15 mm diameter of hydrolysis zone) while L. lactis and Lb. rhamnosus yoba 2012 exhibited low BSH activity (<10 mm diameter of hydrolysis zone). All isolates lacked mucolytic activity. Lb. plantarum MNC 21 and W. confusa MNC 20 produced agmatine. The candidate and reference microorganisms were resistant to 10 of 21 and 5 of 21 antibiotics, respectively. The isolates exhibited hydrophobic, auto-aggregation and coaggregation properties. These three properties were exhibited more (p<0.05) by the reference than the potential probiotics. The ability of the potential probiotics to attach onto the goat ileum (7.3–8.0 log cfu/cm2) was comparable to that of Lb. rhamnosus yoba 2012 (7.6 log cfu/cm2). The four LAB inhibited E. coli, S. aureus, and S. enterica to the same extent (p<0.05). The findings indicated potential probiotic activity of the starter cultures. However, further in vivo examination of these isolates is required to confirm their probiotic capabilities.

Genomic, biochemical and microbial evaluation of probiotic potentials of bacterial isolates from fermented sorghum products

“…The tolerance of some of the isolated strains (including PB2) to acidity and bile salt is vital for bacterial survival and growth in the gastro-intestinal tract. These attributes are the main requirements for bacteria to be considered probiotics [ 44 , 45 ]. Tolerance to bile salts is a prerequisite for colonization and metabolic activity of bacteria in the small intestine of the host [ 46 , 47 ].…”

Technological properties and probiotic potential of Lactiplantibacillus plantarum SJ14 isolated from Algerian traditional cheese “Jben”

“…Based on criterions used to define a probiotic organism, putative probiotic strains should be screened for their functional characteristics (persistence in the gastrointestinal tract, resistance to gastric acidity and bile salts, antimicrobial activity against pathogenic, and adhesion to epithelial cells) and safety properties such as non-pathogenicity and antibiotic resistance characteristics (Byakika et al, 2020). Selection process of probiotic micro-organisms probiotics, must be extended to other criteria such as prevent intestinal disorders, promote antagonistic and antimutagenic properties, improve the immune system and involvement in disease prevention and/or treatment (Abouloifa et al, 2020).…”

Probiotic potential of β‑galactosidase‑producing lactic acid bacteria from fermented milk and their molecular characterization

“…Several studies have reported that 0.3% (w/v) bile salt concentration in the human gut also varies according to diet and the level of pancreatic enzyme secretion ( 33 ). Succi et al ( 34 ) also reported that most of the LAB showed resistance to a 0.5% bile concentration, and Byakika et al ( 35 ) examined whether the probiotic bacteria were tolerant to 1% bile salt. Hsieh et al ( 32 ) also reported that 0.3% bile was used for a bile tolerance test and Lactobacillus TSR332 exhibited the highest viability (4.74x10 8 CFU/ml) indicating high tolerance to bile salt.…”

“…Rashmi and Gayathri ( 13 ) investigated whether gluten-hydrolyzing bacteria adhered to the Caco-2 cell line and inhibited cell invasion by E. coli and L. monocytogenes . Byakika et al ( 35 ) used the goat ileum to assess cell adhesion, and Behbahani et al ( 37 ) used scanning electron microscopy to identify that the adhesion level of the L. plantarum strain L15 to Caco-2 cells was 12%. Aissi et al ( 24 ) employed HT-29, Caco-2 and INT-407 cells, as well as Bifidobacterial strains, and microscopically studied them.…”