Probiotic potential of Lactobacillus paraplantarum BT‐11 isolated from raw buffalo ( Bubalus bubalis ) milk and characterization of bacteriocin‐like inhibitory substance produced

Abstract

This study aimed to isolate bacteriocin‐like inhibitory substance (BLIS) producing strains from raw buffalo milk and characterize their probiotic and antimicrobial potential. The BT‐11 isolate was identified through 16S rDNA gene sequencing and recA gene amplification as Lactobacillus paraplantarum. The BT‐11 strain survived in low pH (2, 3) and bile (0.5, 1, and 2%) for 3 hr of incubation. The strain exhibited adhesion to Caco‐2 cells (69.85%), auto‐aggregation (89.74%), and hydrophobicity (81.76%). The BLIS produced by BT‐11 showed antibacterial activity against indicator bacteria including multidrug‐resistant pathogens. Time–kill kinetic analysis revealed the reduction in the growth of Staphylococcus aureus and Salmonella typhimurium (>3 Log CFU/ml) in time‐dependent manner. BLIS showed biopreservative effect in meat contaminated with Listeria monocytogenes. The results concluded that L. paraplantarum BT‐11 is potential probiotic strain with potential to produce BLISs. Practical applications Raw buffalo milk represents excellent source of unexplored potential probiotics with antimicrobial characteristics. In this study, the Lactobacillus paraplantarum BT‐11 was isolated from raw buffalo milk and its probiotic potential and bacteriocin‐like inhibitory substance (BLIS) was characterized. The results demonstrated that L. paraplantarum BT‐11 is promising potential probiotic. The BLIS produced by L. paraplantarum BT‐11 showed antagonistic activity against pathogens including multidrug‐resistant foodborne bacteria. BLIS also showed biopreservative potential in meat against Listeria monocytogenes. L. paraplantarum and its antimicrobial compound can be used as bio preservative agent in food safety applications.

Antimicrobial Activities and Biopreservation Potential of Lactic Acid Bacteria (LAB) from Raw Buffalo (Bubalus bubalis) Milk

“…One isolate ( L . paraplantarum BT-11) produced bacteriocin-like inhibitory substance (BLIS), which has been already reported by the main author [ 13 ]. Live culture of LAB and their CFS were used against both gram-positive and gram-negative pathogens in several experiments during this study including the biopreservation test to further explore the inhibitory potential of probiotic LAB.…”

“…Lactobacillus paraplantarum 11 (BT), Lactiplantibacillus plantarum 19, Lactobacillus pentosus 42, Limosilactobacillus fermentum 60, Lactobacillus pentosus 93, and Limosilactobacillus reuteri 112, which were previously isolated from buffalo milk were used as probiotic strains in this study [ 11 , 13 ]. The strains were preserved in 20% glycerol and resuscitated in MRS broth.…”

“…The LAB are associated with several health benefits such as improvement of gastrointestinal tract conditions, antibacterial and antifungal activities, antiallergic and antioxidant properties, and lowering cholesterol levels and immunomodulatory activities [ 12 , 13 ]. The LAB produce their antimicrobial activity through the reduction of pathogen translocation, inhibition of pathogen adherence, and production of antimicrobial compounds [ 14 ].…”

“…Lactic acid bacteria and their antimicrobial metabolites can inhibit foodborne pathogens and act as natural biopreservatives. Very limited studies have been reported on biopreservative potential of the probiotics from buffalo milk and their antimicrobial metabolites [ 11 , 13 ]. Therefore, the present study was undertaken to characterize the antimicrobial activity of probiotic LAB isolated from raw buffalo milk and to evaluate their biopreservation potential.…”

“…Therefore, the present study was undertaken to characterize the antimicrobial activity of probiotic LAB isolated from raw buffalo milk and to evaluate their biopreservation potential. [11,13]. The strains were preserved in 20% glycerol and resuscitated in MRS broth.…”