Probiotic potential of autochthonous bacteria from tambaqui Colossoma macropomum

Abstract

In this study, the identification and characterization of potential probiotic bacteria present in natural microbiota of tambaqui Colossoma macromum were performed for the first time. Bacteria from the intestinal mucosa were isolated in agar Man, Rogosa and Sharpe (MRS). Only Gram‐positive and haemolysis‐negative isolates were selected (n = 152). The tests included morphological differentiation, gene sequencing, catalase, bile tolerance, pathogen antagonism, antimicrobial susceptibility and safety to the host. To differentiate the isolates, colonies were morphologically characterized. From 152 initial isolates, 6 strains with probiotic potential were selected and identified through sequencing of 16S rRNA gene of Enterococcus faecalis, Enterococcus hirae, Lactococcus lactis, Pediococcus pentosaceus, Staphylococcus hominis and Staphylococcus saprophyticus. The growth of the six strains was not affected by different levels of bile inclusion. All probiotic strains inhibited the growth of the Lactococcus garvieae pathogen in the antagonism test. When tested against Aeromonas hydrophila, L. lactis and E. hirae strains were prominent. All strains were resistant to some degree against the 11 antibiotics. With this study, it was possible to screen the following six strains with probiotic potential for inclusion in the diet of C. macropomum: S. hominis, S. saprophyticus, L. lactis, P. pentosaceus, E. hirae and E. faecalis.

Probiotic Weissella cibaria KY10 derived from digestive tract of healthy shrimp exhibits strong antibacterial effects against Vibrio parahaemolyticus causing AHPND in shrimp

“…cibaria KY10 was sensitive to tetracycline, chloramphenicol, gentamicin, polymycin B and erythromycin, but resistant to kanamycin, streptomycin and vancomycin (Table 1). Among the antibiotics tested, resistance to vancomycin is a major concern, as it is one of the few antibiotics available to treat infections caused by multidrug‐resistant pathogens (Kotzent et al, 2020). These findings are in agreement with other studies (Choi et al, 2002; Yu, Jang, et al, 2019; Yu, Lee, et al, 2019) concluding that W .…”

Aumento Da Resistência Bacteriana Em Aquacultura

“…Revista Multidisciplinar do Nordeste Mineiro, v.12, 2023ISSN 2178 Atualmente, existem estratégias preventivas que podem ser utilizadas para minimizar as chances de ocorrência dessas infecções zoonóticas e substituir gradativamente a utilização de antibióticos na aquacultura, na qual se destaca a suplementação dietética dos animais com probióticos, prebióticos e simbióticos (Allameh et al, 2017;Yi et al, 2018;Kotzent et al, 2021), uso de fitoterápicos (Schalch et al, 2015;Valladão et al, 2019), a vacinação e a seleção de genótipos resistentes à determinada doença (Longhi et al, 2012;Ariede et al, 2020;Zeng et al, 2021;Miani, 2022). probióticos, prebióticos e simbióticos são definidos como alimentos que estimulam o sistema imunológico dos animais a desenvolver microrganismos benéficos e a consequente diminuição do crescimento dos patogênicos.…”

Synthesis, spectroscopic characterization, and antibacterial activity of chalcone (2E)‐1‐(3′‐aminophenyl)‐3‐(4‐dimethylaminophenyl)‐prop‐2‐en‐1‐one against multiresistant Staphylococcus aureus carrier of efflux pump mechanisms and β‐lactamase

“…Furthermore, the genus Staphylococcus has already been indicated as a promising probiotic [15]; based on this, Ağagündüz et al [16] reported through a review article the promising potential of probiotics as prevention and treatment of certain diseases. The same author further published a review on the role of the microbiome in gastrointestinal cancer, and it was reported that some microorganisms, as well as their metabolites, are able to promote or inhibit tumorigenesis [17].…”