Probiotic Potential of 3 Lactobacilli Strains Isolated From Breast Milk

Abstract

Breast milk is an important factor in the initiation, development, and composition of the neonatal gut microbiota. In a previous study, the authors isolated lactic acid bacteria from milk of healthy mothers. Since some of the identified isolates belonged to the genus Lactobacillus, the objective of this work was to evaluate the probiotic potential of 2 Lactobacillus gasseri and 1 Lactobacillus fermentum strains. Different assays, including survival to conditions simulating those existing in the gastrointestinal tract, production of antimicrobial compounds, adherence to intestinal cells, production of biogenic amines, degradation of mucin, enzymatic profile, and pattern of antibiotic resistance, were performed. Globally, the results showed that the probiotic potential of lactobacilli isolated from milk of healthy mothers is, at least, similar to that of the strains commonly used in commercial probiotic products. This fact, together with the presence of prebiotic substances, indicates that breast milk is a natural synbiotic food.

Early human milk feeding is associated with a lower risk of necrotizing enterocolitis in very low birth weight infants

“…12,27 HM feeding promotes development of an intestinal ecosystem in which bifidobacteria and lactobacilli are the predominate organisms. [28][29][30] These microorganisms interact with the intestine to diminish intestinal inflammatory response to the pathogenic bacteria and toxins that may contribute to the development of NEC. It may be that high HM proportion of enteral feeding in the first 2 weeks assists with reducing inflammation by promoting symbiotic bacterial colonization and/or through other anti-inflammatory properties.…”

Current Issues of Diagnosis and Treatment of Functional Gastrointestinal Disorders in Children

“…Эффективность подобной диетотерапии оценивают через 2 нед, после чего рекомендуется продолжить без-молочную диету у матери [31,32]. При искусственном вскармливании показаны смеси на основе частичного гидролиза молочного белка, обогащенные олигосаха-ридами, эффективность которых ранее была доказа-на [33,34], при этом некоторые авторы считают, что эффективность других формул, например содержащих пробиотики, должна быть доказана в дополнительных исследованиях [35].…”

“…При отсутствии эффекта назначается обследование ребенка с применением эзофагогастро-дуоденоскопии и/или рентгенологического обследо-вания, ультразвукового исследования, биохимических тестов и др. [34].…”

Allomaternal Nursing in Humans

“…Breast milk is a significant factor in the development and structure of the neonatal gut microbiota, and another woman's breast milk may optimize the young infant's immune system, especially in environments with diverse strains of bacteria (Martin et al 2005). The hypothesis that allomaternal nursing contributes to the bacterial colonization of the young infants' digestive system is also worth considering.…”

“…The authors also predict that allomaternal nursing would be more likely in young mammals with high parasite loads (especially macroparasites) or that are infected with virulent pathogens and that mothers should prevent allomaternal nursing when the risk of transmitting microparasites (e.g., viruses, bacteria) is high. They argue that even for women in the same pathogenic environment, different allonursers can provide infants with a stronger immune response, in part by supplying a different antibody (called "paratopes") from the mother for a particular pathogen.Breast milk is a significant factor in the development and structure of the neonatal gut microbiota, and another woman's breast milk may optimize the young infant's immune system, especially in environments with diverse strains of bacteria (Martin et al 2005). The hypothesis that allomaternal nursing contributes to the bacterial colonization of the young infants' digestive system is also worth considering.…”