Probiotic nasal spray development by spray drying

Lung-Directed Bacteriotherapy in Cystic Fibrosis: Could It Be an Option?

“…Resuspension of the powder and dispersion through a nasal spray bottle did not lead to significant viability loss. Finally, functional tests demonstrated that some of the formulations maintained a high capability of adhering to the human airway epithelial cell line Calu-3 as compared to fresh L. casei cells and good antimicrobial activity against common upper respiratory tract pathogens [83]. The study highlights that the final benefits supplied by probiotics might be not only strain-specific but also formulation-and process-dependent.…”

“…Specifically, some nanomaterial-based formulations including nanofibers, nanoparticles and nanocomposites seem to meet most of the requirements for delivery systems of probiotics including efficient encapsulation, controlled release and improved stability of the therapeutic bacteria during manufacturing, storage and in vivo applications [82]. For instance, in a recent study, Jokicevic and coworkers analyzed several spray-dried formulations for probiotic nasal delivery [83]. They screened different combinations of excipients (sugars, sugar-alcohols and polymers) for their ability to preserve the viability of a strain of L. casei after spray drying and during a period of 7 months of shelf-life.…”

Open source anaerobic and temperature-controlled in vitro model enabling real-time release studies with live bacteria

“…However, the increasing occurrence of more complex APIs, for example probiotics (live microorganisms that have a health benefit to the host when ingested in sufficient amounts [3] ), leads to the realization that additional experimental parameters have to be included in the in vitro models. Additionally, probiotics are incorporated in increasingly complex drug delivery systems as an attempt to deliver live bacteria to different sites in the GI tract [4] , [5] , [6] . This increases the need for reliable in vitro methods to study the release of probiotics from such drug delivery systems and ensure that the probiotics stay alive during the in vitro study.…”

Advancements in the Pharmaceutical Applications of Probiotics: Dosage Forms and Formulation Technology

“… 69 , 70 Moreover, Jokicevic et al prepared a nasal formulation of the novel probiotic strain, L. casei AMBR2 derived from the upper respiratory tract (URT) by spray drying that could retain the viability and morphology of probiotic bacteria. 71 This formulation was suitable in terms of the high degree of adherence on Calu-3 cells and antimicrobial activity against upper respiratory tract pathogens during antimicrobial assays with the respiratory cell line. 71 …”

“… 71 This formulation was suitable in terms of the high degree of adherence on Calu-3 cells and antimicrobial activity against upper respiratory tract pathogens during antimicrobial assays with the respiratory cell line. 71 …”