Probiotic microorganisms detoxify ochratoxin A in both a chicken liver cell line and chickens

Abstract

BACKGROUND: The administration of probiotics and prebiotics (synbiotics) is a promising method for detoxification of ochratoxin A (OTA) in animals. The aim of this study was to investigate the ability of five probiotic strains of lactic acid bacteria (LAB) and one Saccharomyces cerevisiae yeast strain, from three different synbiotics for poultry, to detoxify OTA. In addition, we also investigated the genotoxicity of faecal water (FW) of chickens after administering OTA and/or synbiotics for 42 days. RESULTS:All tested LAB and yeast strains had the ability to detoxify OTA by significant (P < 0.05) reducing its concentration (by 31.3-47.7% and 31.9%, respectively, after 24 h incubation) and genotoxicity (by 22.6-51.8% and 52.7%, respectively). Synbiotics composed of four and five probiotic strains significantly (P < 0.05) decreased FW genotoxicity of chicks, after exposure to OTA, to the level seen in the control group (21.8% ± 1.7%) and were more effective than synbiotics composed of three probiotic strains (31.5%). CONCLUSION: These results showed that there was a beneficial effect of the synbiotics on the gastrointestinal tract of animals. Furthermore, synbiotic preparations containing four or five of tested strains can be considered as preventive agents in the contamination of poultry with OTA.

The effect of synbiotic preparations on the intestinal microbiota and her metabolism in broiler chickens

“…Probiotic and synbiotic formulas. Synbiotic 27 . For the examination period, from day 1 to 42 of life, in each group, birds were administered synbiotic preparation A, B or C in a dose of 0.5 g kg −1 of feed and commercial probiotic preparation BioPlus YC or Cylactin in a dose of 0.4 g kg −1 or 0.035 g kg −1 of feed ad libitum respectively.…”

“…The body weight of animals in individual study groups was determined on the first and the 7 th , 14 th , 28 th and 42 sd day of life. Final production parameters such as daily cumulative mortality rate (%), feed conversion ratio (FCR), and European Production Efficiency Factor (EPEF) were determined according to the formulas 27 Determination of intestinal microbiota. The composition of microbiota in intestinal content (the jejunum as part of the small intestine and the caecum) and in the excreta was determined on the 2 nd , 7 th , 14 th , 28 th , and 42 nd day of rearing in seven randomly selected chickens in each experimental group.…”

“…The count of analyzed microorganisms was determined using the culture method in accordance with the PN-ISO standards in triplicate, using selective microbial media 28 . The total anaerobic bacterial count (PCA, Merck), Enterobacteriaceae family bacteria count (VRBD, Merck), Escherichia coli count (TBX, Merck) and the count of bacteria belonging to the genes • Safety and efficacy of B. licheniformis DSM 5749 and B. subtilis DSM 5750 has been confirmed in many studies in vivo [25][26][27] .…”

“…Research results on antagonism of pathogens, adherence to Caco-2, inhibition of adherence of pathogens to Caco-2, antibiotic resistance, resistance to bile salts and low pH, auto-and coaggregation and hydrophobicity are being published (authors: Śliżewska K. and Chlebicz A.). Moreover, additional information about beneficial activities of probiotic strains contained in the synbiotics' composition is available in publications about research in vitro and in vivo (Table 1) [26][27][28] . The comparison of the effect of the newly elaborated preparations with commercial preparations is very important.…”

“…The following parameters of the process were used: Aminex HPX-87H column (300 × 7.8 mm), refractometric RI and UV detector, 0.005 M L −1 sulphuric acid as eluent, flow rate 0.6 μL min −1 , single sample analysis time 40 min.Statistical analysis. The normality of the distribution of variables was tested with Shapiro-Wilk's test, and the homogeneity of variances was examined with Bartlett's test27 . Following the confirmation of normality and equal variance, results were analyzed with a one-way ANOVA test and Tukey's post hoc test.…”