Probiotic-loaded microcapsule system for human in situ folate production: Encapsulation and system validation

Abstract

This study focused on the use of a new system, an alginate|Ɛ-poly-l-lysine|alginate|chitosan microcapsule (APACM), able to immobilize a folate-producing probiotic, Lactococcus lactis ssp. cremoris (LLC), which provides a new approach to the utilization of capsules and probiotics for in situ production of vitamins. LLC is able to produce 95.25±26μg·L of folate, during 10h, and was encapsulated in the APACM. APACM proved its capacity to protect LLC against the harsh conditions of a simulated digestion maintaining a viable concentration of 6logCFU·mLof LLC. A nutrients exchange capacity test, was performed using Lactobacillus plantarum UM7, a high lactic acid producer was used here to avoid false negative results. The production and release of 2g·L of lactic acid was achieved through encapsulation of L. plantarum, after 20h. The adhesion of APACM to epithelial cells was also quantified, yielding 38% and 33% of capsules adhered to HT-29 cells and Caco-2 cells, respectively.

Effect of the formulation and structure of monoglyceride-based gels on the viability of probioticLactobacillus rhamnosusuponin vitrodigestion

“…These results confirmed that the gastric conditions are particularly stressful for microorganisms. 20,26,49,50 In fact, the acidic environment of the stomach could be responsible for the damage of the cell membrane, DNA and cellular proteins leading to the loss of bacterial viability. 51,52 Different results were obtained upon digestion of MG-gels.…”

Microencapsulation of Probiotic Lactobacillus acidophilus KBL409 by Extrusion Technology to Enhance Survival under Simulated Intestinal and Freeze-Drying Conditions

“…In the oral phase, survival rates showed no significant decrease in all treatment groups (0.74-1. to 10 CFU/ml after incubation in simulated gastric juice (pH 2) for 2 and 1 h, respectively, whereas more than 80% Al/Chi-encapsulated cells survived under these conditions [26]. L. lactis encapsulated with alginate-chitosan also showed high viability (75.0%) upon exposure to gastric condition for 2 h, whereas the number of free cells was less than 1 log within 90 min of treatment [27]. Free B. breve were undetected in the presence of gastric condition (pH 2) for 60 min, while the viability of Al/Chi-encapsulated cells (76.8%) further improved [6].…”

Effect of added prebiotic (Isomalto-oligosaccharide) and Coating of Beads on the Survival of Microencapsulated Lactobacillus rhamnosus GG

“…Sequential digestion was adapted from Ramos et al (2016) with slight modifications. To evaluate the survivability of LGG under simulated gastrointestinal condition, 1 g of capsules or 1 mL of free cells were added to 9 mL of sterile simulated gastric juice (SGJ: 7 mL/L 0.1 M HCl, 2 g/L NaCl, 3.2 g/L pepsin) at pH 2.0 and incubated at 37 °C for 0 -2 h (with interval of 1 h) with constant agitation at 100 rpm.…”