Probiotic Lactobacillus spp. Act Against Helicobacter pylori-induced Inflammation

Chen, Yi Hsing; Tsai, Wen‐Hsiang; Wu, Hui; Chen, Chun Ya; Yeh, Wen Ling; Chen, Ya Hui; Hsu, Hui Ying; Chen, Wei Wei; Chen, Yu Wen; Chang, Wen‐Wei; Lin, Tsung-Jen; Lai, Hsin Chih; Lin, Yu Hsin; Lai, Chih Ho

doi:10.3390/jcm8010090

Cited by 87 publications

(71 citation statements)

References 54 publications

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“…Previous studies have shown that the increased Bacteroidetes is associated with dysfunction of innate immune system, resulting in host present in a pro-inflammation state [36], while de ciency of Lactobacillus spp. is involved in gut in ammation and bacterial infection [37]. These results suggest that alteration of microbiota during aging can cause a potent pro-in ammatory effect on aggravating intraintestinal in ammation response, and it may explain why intestinal permeability is increased.…”

Section: Discussionmentioning

confidence: 79%

Age-Related Changes in the Gut Microbiota Promote Atrial Fibrillation

Zhang¹,

Zhang²,

Luo³

et al. 2020

Preprint

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Background: Aging is the most significant contributor to the increasing prevalence of atrial fibrillation (AF). The gut microbiota dysbiosis is involved in age-associated diseases. However, whether age-associated gut microbial dysbiosis contributes to AF is still unknown. The aim of this study was to evaluate the effect of gut microbiota on the susceptibility of aging-induced AF and to elucidate the underlying mechanisms. Results: The gut microbiota profiling of fecal samples in aged (22-24 months old) and young (2-3 months old) rats was performed by 16S ribosomal RNA gene analysis. A rat model of fecal microbiota transplantation (FMT) was established for analyzing the possible role of age-associated gut microbial dysbiosis in AF. Here, we found that aging process led to marked shift of the microbiota spectrum. The microbiota in young rats following FMT resembled that of aged microbiota and transmitted the increased AF susceptibility by elevation of circulating lipopolysaccharide (LPS). The mechanism for LPS-driven atrial pro-arrhythmic action was dependent on the activation of atrial nucleotide binding and oligomerization domain-like receptor family pyrin domain-containing (NLRP3)-inflammasome. Inhibition of inflammasome by MCC950 resulted in lower atrial fibrosis and AF susceptibility. In addition, atrial fibrosis, plasma LPS concentration, plasma glucose to oral glucose tolerance test (OGTT), intestinal permeability, and gut pathology were significantly increased in elderly human subjects. Finally, altering the microbiota in aged recipient to resemble that of young restored the intestinal barrier dysfunction and LPS and impaired glucose tolerance, and the worse outcomes of aged dysbiosis on atrial fibrosis and AF susceptibility were abrogated. Conclusions: These data suggest that age-associated microbial dysbiosis induces circulating LPS and impairs glucose tolerance, and thereby promotes AF susceptibility through enhanced activity of atrial NLRP3-inflammasome.

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Section: Discussionmentioning

confidence: 79%

Age-Related Changes in the Gut Microbiota Promote Atrial Fibrillation

Zhang¹,

Zhang²,

Luo³

et al. 2020

Preprint

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“…It is notable that WMT may be used for refractory H. pylori infection (defined as those who have failed the first eradication treatment or with recurrence of H. pylori infection). Chen et al found that Lactobacillus rhamnosus and Lactobacillus acidophilus had effective antimicrobial activity against multidrug-resistant H. pylori by inhibiting H. pylori -induced inflammation and promoting the growth of probiotics [ 31 ]. WMT, which is also a microbial therapy, may affect the colonization of H. pylori by increasing the abundance of the above probiotics and reducing the inflammation caused by refractory H. pylori infection.…”

Section: Discussionmentioning

confidence: 99%

The Efficacy of Washed Microbiota Transplantation on Helicobacter pylori Eradication: A Pilot Study

Xia

Zhang

et al. 2020

Gastroenterology Research and Practice

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Aim. The fecal microbiota transplantation by washed preparation was recently coined as washed microbiota transplantation (WMT). This pilot study is aimed at exploring the feasibility and efficacy of WMT on Helicobacter pylori eradication. Methods. Consecutive patients who had been treated with WMT for various indications and who were positive for H. pylori infection before WMT treatment but had never received eradication therapy for H. pylori infection were invited to take a follow-up 13C-urea breath test. The associations of demographic, clinical factors, and laboratory indicators for gastric function and intestinal barrier function with the therapeutic effect were determined. Results. A total of 32 eligible patients were included, and the overall H. pylori eradication rate was 40.6% (13/32). Patients with H. pylori eradication had a higher pepsinogen ratio (PGR) than those without ( 13.00 ± 6.97 vs. 8.31 ± 3.733 ; P = 0.02 ). Female patients had a higher, albeit not statistically significant, eradication rate than male patients (53.85% vs. 31.58%; P = 0.208 ). Compared with lower gastrointestinal tract delivery route, middle gastrointestinal tract delivery route seems to be a more suitable way for the treatment of H. pylori infection (58.33% vs 16.67%; P = 0.152 ). There was no significant difference in other demographic and clinical factors between patients with and without H. pylori eradication. Conclusion. H. pylori infection is eradicated in a proportion of patients who have received WMT. An increased pre-WMT PGR appears to be associated with the therapeutic effect. Further studies are required to confirm the efficacy of WMT, especially in combination with currently recommended regimens in randomized controlled trials.

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“…As an emerging adjuvant, probiotics have been used to treat a series of gastrointestinal diseases, including H. pylori infection. In vitro experiments demonstrated that various probiotics have the potential to antagonize H. pylori through their metabolites or bacterial cells [61][62][63]. Sun et al found four Lactobacillus strains isolated from fermented foods in northeastern China were able to inhibit the growth of H. pylori [63].…”

Section: Antagonistic Mechanismmentioning

confidence: 99%

“…Probiotics can inhibit pro-inflammatory factor expression, thereby mitigating the inflammatory response. Numerous studies have shown that probiotic strains such as L. acidophilus, L. bulgaricus, and L. rhamnosus can reduce the expression of IL-8 in H. pylori-infected cells [17,61,80]. Yang et al demonstrated that although H. pylori infection causes the overexpression of IL-8, TNF-α and other pro-inflammatory factors in MKN45 cells, pretreatment with high doses of L. acidophilus La5 can silence the Smad7 and NF-κB pathways, thus relieving the inflammatory response [79].…”

Section: Antagonistic Mechanismmentioning

confidence: 99%

“…After treatment with L. rhamnosus GMNL-74 or L. acidophilus GMNL-185, the abundance of Bifidobacterium spp. and Akkermansia muciniphilia in H. pylori-infected mice was significantly increased [61]. Wu et al found that the diversity of the gut microbiota is remarkably reduced when H. pylori infected individuals are treated with triple therapy alone, while supplementation with Bacillus subtilis and E. faecalis can inhibit this reduction [56].…”

Section: Gastrointestinal Theorymentioning

confidence: 99%

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Using Probiotics as Supplementation for Helicobacter pylori Antibiotic Therapy

Yang

2020

IJMS

105

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Helicobacter pylori is a well-known pathogen that is highly prevalent in the world population, and H. pylori infection is potentially hazardous to humans because of its relationship to various gastrointestinal diseases, such as gastric ulcers, chronic gastritis, and gastric carcinoma. Therefore, the clinical guidelines recommend taking antibiotic therapy to eradicate the pathogen, which usually leads to the desired therapeutic effect. However, some failure cases of this therapy indicate that the increasing antibiotic resistance and side effects may affect the therapeutic effect. Here we propose that using probiotics as supplementation for antibiotic therapy may provide an extra help. Recent studies have shown that probiotic supplementation therapy has promising application prospects; it can enhance the antibiotic effect to achieve a better therapeutic result and maintain the balance of the host gastrointestinal microbiota. In summary, under global conditions of increasing H. pylori prevalence, probiotic supplementation therapy is worthy of further studies for future clinical application.

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Probiotic Lactobacillus spp. Act Against Helicobacter pylori-induced Inflammation

Cited by 87 publications

References 54 publications

Age-Related Changes in the Gut Microbiota Promote Atrial Fibrillation

Age-Related Changes in the Gut Microbiota Promote Atrial Fibrillation

The Efficacy of Washed Microbiota Transplantation on Helicobacter pylori Eradication: A Pilot Study

Using Probiotics as Supplementation for Helicobacter pylori Antibiotic Therapy

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