Probiotic Lactobacillus rhamnosus modulates the gut microbiome composition attenuates preneoplastic colorectal Aberrant crypt foci

Gamallat, Yaser; Ren, Xiaomeng; Walana, Williams; Meyiah, Abdo; Ren, Xiaoliang; Zhu, Yanyan; Li, Meiqi; Song, Siyuan; Xie, Luhan; Jamalat, Yazeed; Saleem, Muhammad Zubair; Ma, Yufang; Xin, Yi; Shang, Dong

doi:10.1016/j.jff.2018.12.018

Cited by 23 publications

(37 citation statements)

References 57 publications

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“…To the best of our knowledge, no study has been conducted to apply the antitumor activity and protective effects of L. gasseri ATCC 33323 on modulating β-catenin expression in gastric epithelial cells stimulated by H. pylori strains. There are only a few reports which used L. rhamnosus (LGG) and L. casei to down-regulate β-catenin expression, thereby preventing the initiation and progression of colon cancer [54,55]. In line with these studies, our results also showed that L. gasseri ATCC 33323 significantly decreased the expression of β-catenin in AGS cells cocultured with clinical H. pylori strains.…”

Section: Discussionsupporting

confidence: 85%

Effects of a Potential Probiotic Strain Lactobacillus gasseri ATCC 33323 on Helicobacter pylori-Induced Inflammatory Response and Gene Expression in Coinfected Gastric Epithelial Cells

Yarmohammadi

Yadegar

Ebrahimi

et al. 2020

Probiotics & Antimicro. Prot.

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In the present study, we aimed to investigate the modulatory effects of a potential probiotic bacterium Lactobacillus gasseri ATCC 33323 on Helicobacter pylori-induced inflammatory response and gene expression in human gastric adenocarcinoma (AGS) cell line. The gastric epithelial cells were coinfected with a collection of H. pylori clinical strains alone or in combination with L. gasseri at a multiplicity of infection (MOI) of 1:100 for each bacterium, and incubated for different time points of 3, 6, and 12 h. IL-8 secretion from coinfected AGS cells after incubation at each time point was measured by an enzyme-linked immunosorbent assay (ELISA). The mRNA expression of IL-8, Bcl-2, β-catenin, integrin α 5 , and integrin β 1 genes was determined by quantitative RT-PCR amplification of total RNA extracted from coinfected epithelial cells. L. gasseri significantly (P < 0.05 and P < 0.01) decreased the production of IL-8 in AGS cells coinfected with H. pylori strains at 6 h post-infection. We also detected that L. gasseri significantly (P < 0.05) down-regulated the gene expression level of IL-8 in H. pylori-stimulated AGS cells after 6 and 12 h of coinfection. Similarly, L. gasseri caused a significant decrease (P < 0.05) in mRNA expression of Bcl-2, β-catenin, integrin α 5 , and integrin β 1 genes in AGS cells at 3 and 6 h after infection with H. pylori strains as compared with non-infected control cells. In conclusion, our results demonstrated that L. gasseri ameliorates H. pylori-induced inflammation and could be developed as a supplementation to the current treatment regimens administrated against H. pylori infection.

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Section: Discussionsupporting

confidence: 85%

Effects of a Potential Probiotic Strain Lactobacillus gasseri ATCC 33323 on Helicobacter pylori-Induced Inflammatory Response and Gene Expression in Coinfected Gastric Epithelial Cells

Yarmohammadi

Yadegar

Ebrahimi

et al. 2020

Probiotics & Antimicro. Prot.

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“…Consumption of probiotics could be one of the therapeutic strategies to improve the homeostasis of intestinal microbiota to ameliorate gastrointestinal diseases caused by gut dysbiosis. The role of probiotics in the alteration of the gut microbiome has been verified clinically in several gastrointestinal diseases such as inflammatory bowel syndrome [4]. For example, it was reported that Lactobacillus rhamnosus, a typical probiotic strain, could reinforce the diversity of microbial composition by modulating the colon microecology and preventing aberrant crypt foci formation in a rat model [4].…”

Section: Introductionmentioning

confidence: 99%

“…The role of probiotics in the alteration of the gut microbiome has been verified clinically in several gastrointestinal diseases such as inflammatory bowel syndrome [4]. For example, it was reported that Lactobacillus rhamnosus, a typical probiotic strain, could reinforce the diversity of microbial composition by modulating the colon microecology and preventing aberrant crypt foci formation in a rat model [4]. Lactic acid bacteria (LAB) are major strains of microflora in Asian fermented foods such as Japanese natto, Korean makgeolli, cheonggukjang, and kimchi.…”

Section: Introductionmentioning

confidence: 99%

Probiotic and Antioxidant Properties of Novel Lactobacillus brevis KCCM 12203P Isolated from Kimchi and Evaluation of Immune-Stimulating Activities of Its Heat-Killed Cells in RAW 264.7 Cells

Song¹,

Jang²,

Kim³

et al. 2019

Journal of Microbiology and Biotechnology

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The purpose of this study was to determine the probiotic properties of Lactobacillus brevis KCCM 12203P isolated from the Korean traditional food kimchi and to evaluate the antioxidative activity and immune-stimulating potential of its heat-killed cells to improve their bio-functional activities. Lactobacillus rhamnosus GG, which is a representative commercial probiotic, was used as a comparative sample. Regarding probiotic properties, L. brevis KCCM 12203P was resistant to 0.3% pepsin with a pH of 2.5 for 3 h and 0.3% oxgall solution for 24 h, having approximately a 99% survival rate. It also showed strong adhesion activity (6.84%) onto HT-29 cells and did not produce β-glucuronidase but produced high quantities of leucine arylamidase, valine arylamidase, β-galactosidase, and N-acetyl-βglucosaminidase. For antioxidant activity, it appeared that viable cells had higher radical scavenging activity in the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay, while in the 2azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay, heat-killed cells had higher antioxidant activity. Additionally, L. brevis KCCM 12203P showed higher lipid oxidation inhibition ability than L. rhamnosus GG; however, there was no significant difference (p < 0.05) between heat-killed cells and control cells. Furthermore, heat-killed L. brevis KCCM 12203P activated RAW 264.7 macrophage cells without cytotoxicity at a concentration lower than 10 8 CFU/ml and promoted higher gene expression levels of inducible nitric oxide synthase, interleukin-1β, and interleukin-6 than L. rhamnosus GG. These results suggest that novel L. brevis KCCM 12203P could be used as a probiotic or applied to functional food processing and pharmaceutical fields for immunocompromised people.

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“…Lactobacillus rhamnosus GG (LGG) is widely recognized for its inhibitory effects on colon aberrant crypt foci, and these effects are mediated by modification of the gut microbiota, upregulation of the protein expression of Bax, P53, Caspase‐3, IL‐2, IFN‐γ, and downregulation of the expression of COX‐2, Bcl‐2, IL‐10, β catenin, tumor necrosis factor (TNF)α, and NF‐κB in rats supplemented with dimethylhydrazine 20 . Recently, it was found that oral administration of Lactobacillus casei BL23 strain in an azoxymethane (AOM)/DSS‐induced C57BL/6 mouse model can inhibit CRC by downregulating the expression of IL‐22 cytokine and upregulating the expression of caspase‐7, caspase‐9, and correcting CRC‐related dysbiosis 21 .…”

Section: Intervention On Gut Microbiota Composition Using Probiotics Prebiotics and Dietsmentioning

confidence: 99%

Intervention on gut microbiota may change the strategy for management of colorectal cancer

Taghinezhad-S

Mohseni

2021

J of Gastro and Hepatol

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Dysbiosis in the gut microbiota composition due to environmental or genetic variations can disrupt the immune system and may promote several diseases such as colorectal cancer (CRC). Gut microbiota can alter the toxicity and efficiency of an extensive range of CRC treatment methods, especially surgery, chemotherapy, radiotherapy, and immunotherapy. The recent scientific evidence suggested that gut microbiota modulation exhibits an essential positive influence on inhibition and treatment of CRC. The literature survey revealed that modulating the gut microbiota composition by probiotics, prebiotics, and diets protects CRC patients from treatment‐associated adverse effects. This review summarizes the recent advancements in the association between interventions on gut microbiota and CRC to provide innovative strategies for enhancing the safety and efficiency of CRC therapy.

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Probiotic Lactobacillus rhamnosus modulates the gut microbiome composition attenuates preneoplastic colorectal Aberrant crypt foci

Cited by 23 publications

References 57 publications

Effects of a Potential Probiotic Strain Lactobacillus gasseri ATCC 33323 on Helicobacter pylori-Induced Inflammatory Response and Gene Expression in Coinfected Gastric Epithelial Cells

Effects of a Potential Probiotic Strain Lactobacillus gasseri ATCC 33323 on Helicobacter pylori-Induced Inflammatory Response and Gene Expression in Coinfected Gastric Epithelial Cells

Probiotic and Antioxidant Properties of Novel Lactobacillus brevis KCCM 12203P Isolated from Kimchi and Evaluation of Immune-Stimulating Activities of Its Heat-Killed Cells in RAW 264.7 Cells

Intervention on gut microbiota may change the strategy for management of colorectal cancer

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