Probiotic Lactobacillus paracasei A221 improves the functionality and bioavailability of kaempferol-glucoside in kale by its glucosidase activity

Abstract

The interplay between food components and gut microbiota has been considered an important factor affecting the functionality of health-promoting foods. In this study, the effects of the probiotic Lactobacillus paracasei A221 on the functionality and bioavailability of kaempferol-3-o-sophroside (KP3S), a kaempferol-glucoside contained in kale, were investigated in vitro and in vivo. Unlike the type strain NBRC15889, the A221 strain converted standard KP3S as well as the kaempferol-glucosides in kale extract into kaempferol (KP). Using an intestinal barrier model, treatment with A221 significantly improved the effects of kale extract on the barrier integrity in vitro. KP, but not KP3S, clearly induced similar effects, suggesting that KP contributes to the functional improvement of the kale extract by A221. Pharmacokinetics analyses revealed that the co-administration of A221 and KP3S significantly enhanced the amount of deconjugated KP in murine plasma samples at 3 h post-administration. Finally, the oral administration of KP to Sod1-deficinet mice, which is a good mouse model of age-related disease, clearly ameliorated various pathologies, including skin thinning, fatty liver and anemia. These findings suggest that Lactobacillus paracasei A221 is effective for enhancing the anti-aging properties of kaempferol-glucosides by modulating their functionality and bioavailability through the direct bioconversion.

Antioxidant, Gastroprotective, Cytotoxic Activities and UHPLC PDA-Q Orbitrap Mass Spectrometry Identification of Metabolites in Baccharis grisebachii Decoction

“…Peak 12 was identified as rutin (C 27 H 29 O 16 ) and peak 13 has been characterized as the methylated quercetin derivative isorhamnetin (C 16 H 11 O 7 ), while peak 10 showing a parent ion at m / z : 447.09088 and a daughter ion at m / z : 285.03894 (kaempferol) as a kaempferol hexoside, possibly kaempferol 3- O -glucoside or kaempferol 3- O -galactoside, the first one has been regarded as antiaging compound [40] and affects the endothelial function of Ginkgo biloba extract [41] and the second prevented carbon tetrachloride-induced liver injury in mice, being regarded as a liver protective compound [42]. Peak 24 was tentatively identified as the demethylated rhamnacin (3,7-dimethyl quercetin, C 17 H 13 O 7 ), and peak 29 as nevadesin (5,7-dihydroxy-6,8,4′-trimethoxyflavone) which has a variety of pharmacological effects such as anti-mycobacterium tuberculosis, antitussive, anti-inflammatory, antihypertensive and free radical-scavenging activities effects [43,44,45].…”

Bioprocessing of common pulses changed seed microstructures, and improved dipeptidyl peptidase-IV and α-glucosidase inhibitory activities

“…L. plantarum ATCC8014 indeed exhibited high β-glucosidase activity 48 . Deglycosylation and biotransformation by lactic acid bacteria was reported to significantly improve intestinal barrier integrity in vitro , and flavonoid bioavailability in vivo 49 . Similarly, in our study, kaempferol glycosides in green lentils were deglycosylated during SSF with L. plantarum (Table 2).…”

Ginsenoside Compound K: Insights into Recent Studies on Pharmacokinetics and Health-Promoting Activities

“…This is the first research on Japanese subjects which evaluates the health properties of P. ginseng . The positive bio transforming role of L. paracasei A221 modulating bioavailability and functional aspects has also been described [ 25 ]. In recent research, LC-MS was used to spot 13 ginsenosides in the human plasma following two-week recurrent supplementation of red ginseng extract.…”