Probiotic Lactobacillus casei activates innate immunity via NF-κB and p38 MAP kinase signaling pathways

Kim, Yun Gi; Ohta, Toshihisa; Takahashi, Takuya; Kushiro, Akira; Nomoto, Koji; Yokokura, Teruo; Okada, Nobuhiko; Danbara, Hirofumi

doi:10.1016/j.micinf.2005.10.019

Cited by 101 publications

(84 citation statements)

References 58 publications

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“…to shift cellular immune responses toward Th1 may be advantageous for vaccination strategies, as was recently shown in a model of pneumococcal infection (26). Lactobacillus species have several components that elicit innate immune responses through molecular pattern recognition receptors of mammalian cells, such as peptidoglycan, lipoteichoic acids, and bacterial oligodeoxynucleotides (29,54). Furthermore, there is evidence that some Lactobacillus strains per se can have an adjuvant effect (27).…”

Section: Discussionmentioning

confidence: 99%

Oral Immunization with RecombinantLactobacillus plantarumInduces a Protective Immune Response in Mice with Lyme Disease

Río

Dattwyler

Aroso

et al. 2008

Clin Vaccine Immunol

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Mucosal immunization is advantageous over other routes of antigen delivery because it can induce both mucosal and systemic immune responses. Our goal was to develop a mucosal delivery vehicle based on bacteria generally regarded as safe, such as Lactobacillus spp. In this study, we used the Lyme disease mouse model as a proof of concept. We demonstrate that an oral vaccine based on live recombinant Lactobacillus plantarum protects mice from tick-transmitted Borrelia burgdorferi infection. Our method of expressing vaccine antigens in L. plantarum induces both systemic and mucosal immunity after oral administration. This platform technology can be applied to design oral vaccine delivery vehicles against several microbial pathogens.Lactic acid bacteria are naturally associated with mucosal surfaces, particularly the gastrointestinal tract, and are also indigenous to food-related habitats, including plants, wine, milk, and meat. These gram-positive bacteria include both important pathogens, e.g., several Streptococcus species, and extremely valuable nonpathogenic species that have been used since ancient times for food and feed fermentation (11,37,58). The host is highly adapted to the presence of commensal intestinal bacteria (36). There is evidence that some strains of lactic acid bacteria have a favorable influence on physiologic and pathological processes of the host due to their specific health-promoting probiotic characteristics that relate to modulation of the immune system (15,36,41). Some strains of lactic acid bacteria polarize the naïve immune system by skewing it toward Th1 responses (41, 56).There have been a number of reports of oral vaccine candidates established from genetically modified pathogenic bacteria, such as Salmonella and Listeria species (1, 2, 30, 45, 52), or commensal bacteria, such as Lactococcus lactis and Lactobacillus species (27,31,32,60). The latter are food-grade bacteria that have GRAS status (generally regarded as safe). While both pathogenic and commensal bacteria have advantages and disadvantages as mucosal delivery vehicles, lactic acid bacteria are preferable in terms of safety and a lower risk of side effects (27,47). Presentation of antigens on the surface of lactobacilli is attractive for vaccine design, especially because the peptidoglycan layer of some strains appears to exhibit natural immuno-adjuvanticity (33,35,44,46). Thus, these species are excellent candidates for the development of safe mucosal delivery vehicles of prophylactic and therapeutic molecules. Of the Lactobacillus strains previously used for vaccine delivery, we chose L. plantarum because there is evidence that this strain is a better agent for vaccination with tetanus toxin fragment C (TTFC) than L. casei or L. lactis (22,53).Lyme disease is the most prevalent vector-borne infectious disease in the United States. A vaccine administered via needle inoculation and based on outer surface protein A (OspA) has proved effective in preventing Borrelia burgdorferi infection in animals and humans (7,10,(16)(17)...

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Section: Discussionmentioning

confidence: 99%

Oral Immunization with RecombinantLactobacillus plantarumInduces a Protective Immune Response in Mice with Lyme Disease

Río

Dattwyler

Aroso

et al. 2008

Clin Vaccine Immunol

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“…It also has been shown that defective activation of ERK is responsible for elevated production of IL-12 by macrophages in response to Helicobacter hepaticus (20). As for the cytokine response of phagocytes to probiotic bacteria, the roles of MAPKs, especially ERK, have not yet been fully elucidated, although some reports showed the importance of the MAPK pathways in the regulation of cytokine production in response to probiotics as well as other bacteria (21)(22)(23)(24). Little is known regarding the receptors and active components of probiotics involved in MAPK activation.…”

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confidence: 99%

Bacterial Teichoic Acids Reverse Predominant IL-12 Production Induced by Certain Lactobacillus Strains into Predominant IL-10 Production via TLR2-Dependent ERK Activation in Macrophages

Kaji

Kiyoshima-Shibata

Nagaoka

et al. 2010

The Journal of Immunology

162

119

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“…Total RNA from each sample was subjected to reverse-transcriptase to cDNA using a SuperScript VILO cDNA Synthesis Kit. qPCR was performed using Power SYBR Green PCR Master Mix containing the following primers: TLR-4 [33], SR-MARCO, CD36, SR-BI, MMP-9, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) [34]. Relative quantification of gene expression was determined using a comparative threshold (CT) method.…”

Section: Rna Isolation and Rt-qpcr Assaymentioning

confidence: 99%

Original article. Patterns of microglial innate immune responses elicited by amyloid β_1–42and lipopolysaccharide: the similarities of the differences

Boondam

Cheepsunthorn

2014

Asian Biomedicine

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Background: As part of their innate immune response to changes in the central nervous system environment, normally quiescent microglia become activated and increase expression of pattern recognition receptors, scavenger receptors, and production of inflammatory cytokines, proteinases, reactive oxygen species (ROS), and free radicals. These molecules have been implicated in the pathogenesis and progression of several neurodegenerative disorders including Alzheimer disease (AD).Objective: We compared patterns of microglial innate immune responses elicited by nonfibrillar amyloid β peptide (nfAβ1-42) to those elicited by lipopolysaccharide (LPS).Methods: Murine BV-2 microglial cells were exposed to either nfAβ1-42or LPS for 12 h. Then, total RNA from each condition was isolated and expression levels of Toll-like receptor (TLR)-4, scavenger receptor class A (SRMARCO) and class B (SR-BI), CD36, and matrix metalloproteinase (MMP)-9 were determined by reverse transcription-quantitative real-time polymerase chain reaction. The amount of hydrogen peroxide (H2O2) and nitric oxide (NO) in the cell-free supernatant at 24 h were determined using 10-acetyl-3,7-dihydroxyphenoxazine (Amplex Red) and Griess reagent, respectively.Results: nfAβ1-42and LPS significantly increased expression of TLR-4, SR-MARCO, CD36, and MMP-9 and production of H2O2 and NO in BV-2 microglial cells compared with that of unstimulated cells. However, expression of SR-BI was significantly induced only when the cells were exposed to nfAβ1-42.Conclusion: These findings indicate that pattern of microglial innate immune responses elicited by nfAβ1-42overlap with that elicited by LPS and suggest a specific role of microglial SR-BI expression in AD pathogenesis.

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Probiotic Lactobacillus casei activates innate immunity via NF-κB and p38 MAP kinase signaling pathways

Cited by 101 publications

References 58 publications

Oral Immunization with RecombinantLactobacillus plantarumInduces a Protective Immune Response in Mice with Lyme Disease

Oral Immunization with RecombinantLactobacillus plantarumInduces a Protective Immune Response in Mice with Lyme Disease

Bacterial Teichoic Acids Reverse Predominant IL-12 Production Induced by Certain Lactobacillus Strains into Predominant IL-10 Production via TLR2-Dependent ERK Activation in Macrophages

Original article. Patterns of microglial innate immune responses elicited by amyloid β_1–42and lipopolysaccharide: the similarities of the differences

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Probiotic Lactobacillus casei activates innate immunity via NF-κB and p38 MAP kinase signaling pathways

Cited by 101 publications

References 58 publications

Oral Immunization with RecombinantLactobacillus plantarumInduces a Protective Immune Response in Mice with Lyme Disease

Oral Immunization with RecombinantLactobacillus plantarumInduces a Protective Immune Response in Mice with Lyme Disease

Bacterial Teichoic Acids Reverse Predominant IL-12 Production Induced by Certain Lactobacillus Strains into Predominant IL-10 Production via TLR2-Dependent ERK Activation in Macrophages

Original article. Patterns of microglial innate immune responses elicited by amyloid β1–42and lipopolysaccharide: the similarities of the differences

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Original article. Patterns of microglial innate immune responses elicited by amyloid β_1–42and lipopolysaccharide: the similarities of the differences