Probiotic genomic DNA reduces the production of pro-inflammatory cytokine tumor necrosis factor-alpha

Kim, Chung Hee; Kim, Han Geun; Kim, Joo Yun; Kim, Na Ra; Jung, Bong Jun; Jeong, Ji Hye; Chung, Dae Kyun

doi:10.1111/j.1574-6968.2011.02470.x

Cited by 41 publications

(20 citation statements)

References 23 publications

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“…frailty (Claesson et al, 2012), sarcopenia (Rampelli et al, 2013), cognitive impairment and AD (Porter et al, 2000; Widner et al, 1999)) by reduced production of short-chain fatty acids (SCFAs) which have a powerful immunoregulatory activity (Shapiro et al, 2014). Probiotics have the potential to rebalance gut microbiota and modulate gut immune response inhibiting the NF-κB pathway (Kim et al, 2012). Our findings suggest a significant moderate to large effect size of probiotics supplementation on reduction of inflammatory biomarkers, but it should be considered that this result could be overestimated by a significant small-study effect, at least for IL-6 studies.…”

Section: Discussionmentioning

confidence: 99%

Evidence-based nutritional and pharmacological interventions targeting chronic low-grade inflammation in middle-age and older adults: A systematic review and meta-analysis

Custodero

Mankowski

Lee

et al. 2018

Ageing Research Reviews

124

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Growing evidence suggests chronic low-grade inflammation (LGI) as a possible mechanism underlying the aging process. Some biological and pharmaceutical compounds may reduce systemic inflammation and potentially avert functional decline occurring with aging. The aim of the present meta-analysis was to examine the association of pre-selected interventions on two established biomarkers of inflammation, interleukin-6 (IL-6), and C-reactive protein (CRP) in middle-age and older adults with chronic LGI. We reviewed the literature on potential anti-inflammatory compounds, selecting them based on safety, tolerability, acceptability, innovation, affordability, and evidence from randomized controlled trials. Six compounds met all five inclusion criteria for our systematic review and meta-analysis: angiotensin II receptor blockers (ARBs), metformin, omega-3, probiotics, resveratrol and vitamin D. We searched in MEDLINE, PubMed and EMBASE database until January 2017. A total of 49 articles fulfilled the selection criteria. Effect size of each study and pooled effect size for each compound were measured by the standardized mean difference. I2 was computed to measure heterogeneity of effects across studies. The following compounds showed a significant small to large effect in reducing IL-6 levels: probiotics (−0.68 pg/ml), ARBs (−0.37 pg/ml) and omega-3 (−0.19 pg/ml). For CRP, a significant small to medium effect was observed with probiotics (−0.43 mg/L), ARBs (−0.2 mg/L), omega-3 (−0.17 mg/L) and metformin (−0.16 mg/L). Resveratrol and vitamin D were not associated with any significant reductions in either biomarker. These results suggest that nutritional and pharmaceutical compounds can significantly reduce established biomarkers of systemic inflammation in middle-age and older adults. The findings should be interpreted with caution, however, due to the evidence of heterogeneity across the studies.

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Section: Discussionmentioning

confidence: 99%

Evidence-based nutritional and pharmacological interventions targeting chronic low-grade inflammation in middle-age and older adults: A systematic review and meta-analysis

Custodero

Mankowski

Lee

et al. 2018

Ageing Research Reviews

124

View full text Add to dashboard Cite

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“…Several groups have explored the immunomodulatory potential of Lactobacillus genomic DNA. Kim and colleagues [2012] reported that pretreatment of monocytic THP-1 cell line with L. plantarum gDNA inhibited production of TNFα in response to LPS. Likewise, Bouladoux and colleauges [2012] have identified unique immunosuppressive motifs enriched in the DNA of Lactobacillus species that inhibit activation of dendritic cells in the gastrointestinal tract.…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus priming of the respiratory tract: Heterologous immunity and protection against lethal pneumovirus infection

Garcia-Crespo¹,

Chan²,

Gabryszewski³

et al. 2013

Antiviral Research

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We showed previously that wild-type mice primed via intranasal inoculation with live or heat-inactivated Lactobacillus species were fully (100%) protected against the lethal sequelae of infection with the virulent pathogen, pneumonia virus of mice (PVM), a response that is associated with diminished expression of proinflammatory cytokines and diminished virus recovery. We show here that 40% of the mice primed with live Lactobacillus survived when PVM challenge was delayed for 5 months. This robust and sustained resistance to PVM infection resulting from prior interaction with an otherwise unrelated microbe is a profound example of heterologous immunity. We undertook the present study in order to understand the nature and unique features of this response. We found that intranasal inoculation with L. reuteri elicited rapid, transient neutrophil recruitment in association with proinflammatory mediators (CXCL1, CCL3, CCL2, CXCL10, TNF-alpha and IL-17A) but not Th1 cytokines. IFNγ does not contribute to survival promoted by Lactobacillus-priming. Live L. reuteri detected in lung tissue underwent rapid clearance, and was undetectable at 24 hrs after inoculation. In contrast, L. reuteri peptidoglycan (PGN) and L. reuteri genomic DNA (gDNA) were detected at 24 and 48 hours after inoculation, respectively. In contrast to live bacteria, intranasal inoculation with isolated L. reuteri gDNA elicited no neutrophil recruitment, had minimal impact on virus recovery and virus-associated production of CCL3, and provided no protection against the negative sequelae of virus infection. Isolated PGN elicited neutrophil recruitment and proinflammatory cytokines but did not promote sustained survival in response to subsequent PVM infection. Overall, further evaluation of the responses leading to Lactobacillus-mediated heterologous immunity may provide insight into novel antiviral preventive modalities.

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“…However, p-gDNA inhibits LPS-induced TNF-α production by THP-1 cells. Furthermore, p-gDNA reduces the expression of TLR2, TLR4 and TLR9, which induces the activation of NF-ĸB through the LPS signaling pathway, leading to the upregulation of inflammatory cytokines [153,154]. Pretreatment of p-gDNA inhibited the phosphorylation of MAPKs and NF-ĸB, and also inhibited LPS-induced TNF-α production in subsequent LPS stimulation.…”

Section: Mechanisms Of Action Of Probioticsmentioning

confidence: 99%

“…Pretreatment of p-gDNA inhibited the phosphorylation of MAPKs and NF-ĸB, and also inhibited LPS-induced TNF-α production in subsequent LPS stimulation. In this regard, L. plantarum genomic DNA-mediated inhibition of signaling and TNF-α was accompanied by the suppression of TLR2, TLR4 and TLR9, as well as the induction of IL-1 receptor-associated kinase M (a negative regulator of TLR) [154]. …”

Section: Mechanisms Of Action Of Probioticsmentioning

confidence: 99%

Probiotic Mechanisms of Action

et al. 2012

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Probiotics are live microorganisms that provide health benefits to the host when ingested in adequate amounts. The strains most frequently used as probiotics include lactic acid bacteria and bifidobacteria. Probiotics have demonstrated significant potential as therapeutic options for a variety of diseases, but the mechanisms responsible for these effects have not been fully elucidated yet. Several important mechanisms underlying the antagonistic effects of probiotics on various microorganisms include the following: modification of the gut microbiota, competitive adherence to the mucosa and epithelium, strengthening of the gut epithelial barrier and modulation of the immune system to convey an advantage to the host. Accumulating evidence demonstrates that probiotics communicate with the host by pattern recognition receptors, such as toll-like receptors and nucleotide-binding oligomerization domain-containing protein-like receptors, which modulate key signaling pathways, such as nuclear factor-ĸB and mitogen-activated protein kinase, to enhance or suppress activation and influence downstream pathways. This recognition is crucial for eliciting measured antimicrobial responses with minimal inflammatory tissue damage. A clear understanding of these mechanisms will allow for appropriate probiotic strain selection for specific applications and may uncover novel probiotic functions. The goal of this systematic review was to explore probiotic modes of action focusing on how gut microbes influence the host.

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Probiotic genomic DNA reduces the production of pro-inflammatory cytokine tumor necrosis factor-alpha

Cited by 41 publications

References 23 publications

Evidence-based nutritional and pharmacological interventions targeting chronic low-grade inflammation in middle-age and older adults: A systematic review and meta-analysis

Evidence-based nutritional and pharmacological interventions targeting chronic low-grade inflammation in middle-age and older adults: A systematic review and meta-analysis

Lactobacillus priming of the respiratory tract: Heterologous immunity and protection against lethal pneumovirus infection

Probiotic Mechanisms of Action

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