Probiotic features of two oral Lactobacillus isolates

Abstract

In this study, we checked lactobacilli strains of human origin for their potential as probiotic. Samples were collected from oral mucosa of 16 healthy individuals, out of which twenty isolates were obtained and two of them were selected and identified as Lactobacillus plantarum (G1) and L. casei (G3). Both isolates exhibited antagonistic action towards pathogenic microorganisms such as Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella abony, and Clostridium sporogenes, but not on the growth of Candida albicans. The bacteriocin activity against Staphylococcus aureus ATCC 6358-P was shown only by L. plantarum G1. Moreover, the isolates G1 and G3 showed good viability in the acid gastric environment and in the gut environment containing bovine bile salts. The viability of G1 and G3 isolates in the gastrointestinal tract, and the adhesion to the intestinal mucosa were also confirmed in vivo. The biochemical tests of blood samples revealed lower levels of serum triglycerides and cholesterol, as well as reduced activity of alkaline phosphatase in all lactobacilli-treated Wistar rats, compared to control ones. No toxicity for NMRI Ham mice was observed. According to our experimental results, these findings imply that L. plantarum G1 and L. casei G3 could be characterized as potential probiotics

Quality Assessment of Bee Pollen: A Cross Sectional Survey in Bulgaria

“…It is also important that in all dry bee pollen samples, the total aerobic plate count possessed less than 100000 CFU/g (Campos et al, 2008). Lactic acid bacteria are known to cause food spoilage such as milk fermentation (Zavisic et al, 2012). The growth of Gram-positive, facultative anaerobes such as Lactobacilli in the pollen samples of the current survey is similar with the findings of Belhadj et al (2014) who isolated Lactobacillus plantarum, L. fermentum, Lactococcus lactis, L. ingluviei, Pediococcus pentosaceus, L. acidipiscis, and Weissella cibaria from raw bee pollen grains collected in some Algerian areas.…”

Probiotics for Prevention and Treatment of Candidiasis and Other Infectious Diseases: Lactobacillus spp. and Other Potential Bacterial Species

“…Their effects on intestinal microbiota in terms of protection include competition for adhesion sites with pathogenic microorganisms and antimicrobial substance production, such as organic acids, lactic acid, carbon dioxide, and bacteriocins [41]. In addition, the regular use of probiotic appears to prevent certain gastrointestinal disorders such as lactose intolerance [42].…”

Short communication: Survival of the characteristic microbiota in probiotic fermented camel, cow, goat, and sheep milks during refrigerated storage

“…The numerous health benefits reported for lactobacilli and bifidobacteria include reduction in lactose intolerance in some individuals, treatment and prevention of diarrhea, alleviation of constipation, contribution to faster recolonization of the intestinal microbiota after administration of antibiotics, possible treatment of inflammatory bowel disease, reduction in serum cholesterol level, increased resistance to microbial infections, effect on immune function, and potential role in cancer prevention (Möller and de Vrese, 2004;Leahy et al, 2005;Zavisic et al, 2012). However, the therapeutic effects exerted by probiotic bacteria are dependent on the number of viable microbial cells reaching the human gut (Leahy et al, 2005;Ghoddusi and Hassan, 2011).…”