Probiotic characterization of indigenous Bacillus velezensis strain DU14 isolated from Apong, a traditionally fermented rice beer of Assam

Borah, Twinkle; Gogoi, Bhargab; Khataniar, Ankita; Gogoi, Madhurjya; Das, Aparoop; Borah, Debajit

doi:10.1016/j.bcab.2019.01.046

“…The spectrum of exopolysaccharide was composed of the following bands at: (1) 1091.51 and 1095.37 cm −1 (corresponding with C–H bending vibrations); (2) 1430.92 cm −1 (attributed to CH 2 symmetrical stretching); (3) 1656.55 cm −1 (the –C=C stretching vibrations); (4) 3322.75 cm −1 (the C=C–H group stretching) [ 35 ]. The spectrum of the kaolinite particles obtained after EPS adsorption contained two specific bands, typical for selected macromolecular compound.…”

Section: Resultsmentioning

confidence: 99%

Impact of Sinorhizobium meliloti Exopolysaccharide on Adsorption and Aggregation in the Copper(II) Ions/Supporting Electrolyte/Kaolinite System

Szewczuk-Karpisz

¹

,

Tomczyk

²

,

Komaniecka

³

et al. 2021

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To obtain insight into physicochemical interactions between Cu(II) ions, kaolinite, and exopolysaccharide (EPS) synthesized by Sinorhizobium meliloti Rm 1021 soil bacteria, an adsorption, electrokinetic, and aggregation study was performed in the selected systems. The obtained data showed that supporting electrolyte type affects both EPS and Cu(II) ions adsorption. For initial Cu(II) concentration 100 mg/L, 4.36 ± 0.25 mg/g (21.80 ± 1.00%) of the ions were adsorbed in 0.001 M NaCl and 3.76 ± 0.20 mg/g (18.80 ± 1.00%) in 0.001 M CaCl2. The experimental data were best fitted to the Langmuir model as well as pseudo second-order equation. The EPS adsorbed amount on kaolinite was higher in the CaCl2 electrolyte than in NaCl one. For an initial polymer concentration of 100 mg/L, the EPS adsorbed amount was 4.69 ± 0.08 mg/g (23.45 ± 0.40%) in 0.001 M NaCl and 5.26 ± 0.15 mg/g (26.32 ± 0.75%) in 0.001 M CaCl2. In the mixed system, regardless of electrolyte type, exopolysaccharide contributed to immobilization of higher amount of copper(II) ions on the clay mineral. Also, in the samples containing heavy metal ions and exopolysaccharide simultaneously, the aggregation of kaolinite particles was the strongest. The results presented in the paper may be very helpful in soil bioremediation, especially in the development of technologies reducing the mobility of heavy metals in the environment.

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“…Due to its rich metabolites and wide distribution in nature, B. velezen-sis has become a new strain used in aquaculture in recent years [9]. The antimicrobial properties of probiotics were considered one of the criteria for application to aquatic animal feed [36]. Secondary metabolites produced by B. velezensis include bacilysin, difficidin, macrolactin, acetoin, and others [37].…”

Section: Discussionmentioning

confidence: 99%

Effects of Dietary Bacillus velezensis LSG2-5 on Growth, Immunity, Antioxidant Capacity, and Disease Resistance of Amur minnow (Rhynchocypris lagowskii Dybowski)

Zhang

¹

,

Yu

²

,

Lin³

et al. 2022

Aquaculture Nutrition

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The study was aimed at investigating the effects of Bacillus velezensis LSG2-5 isolated from host’s intestinal tract on growth performance, immune response, antioxidant capacity, and disease resistance of Amur minnow (Rhynchocypris lagowskii Dybowski). 535 fish were divided into 5 groups with 3 replicates per group. Diets containing different doses (0, 106, 107, 108, and 109 CFU/g, respectively) of B. velezensis LSG2-5 were used to feed fish ( 9.95 ± 0.05 g) for 56 days. Results showed that compared with control, weight gain rate and specific growth rate of fish were significantly increased by dietary 107-109 CFU/g B. velezensis ( p < .05 ). While for the feed conversion ratio, all treatment groups were significantly decreased ( p < .05 ). In serum, the contents of lysozyme and immunoglobulin M were significantly increased by dietary 106-109 CFU/g B. velezensis ( p < .05 ); the activities of component 3, component 4, and alkaline phosphatase were significantly increased by dietary 108 CFU/g B. velezensis ( p < .05 ). The activities of catalase, glutathione peroxidase, total antioxidant capacity, and superoxide dismutase in hepatopancreas were significantly increased by dietary 108-109 CFU/g B. velezensis ( p < .05 ). The activities of catalase, glutathione, glutathione peroxidase, total antioxidant capacity, and superoxide dismutase in the intestinal tract of 108 CFU/g group were significantly increased ( p < .05 ). However, malondialdehyde levels in the hepatopancreas and intestines were significantly decreased by dietary 107-108 CFU/g B. velezensis ( p < .05 ). Dietary 107-109 CFU/g B. velezensis significantly upregulated catalase, glutathione peroxidase, and copper/zinc superoxide dismutase mRNA levels ( p < .05 ). The levels of transforming growth factor β and interleukin-10 mRNA in the hepatopancreas of fish fed with B. velezensis of 108 CFU/g were significantly increased ( p < .05 ), and these in the intestinal tract of 106-109 CFU/g groups were upregulated ( p < .05 ). Feeding dietary 107-108 CFU/g B. velezensis significantly downregulated interleukin-8, interleukin-1β, and tumour necrosis factor α mRNA levels in hepatopancreas and intestines ( p < .05 ). After 56 days of feeding, 225 fish ( 23.20 ± 0.59 g) were challenged with Aeromonas hydrophila (108 CFU/g), and survival rates were recorded for the next 14 days. Meanwhile, the serum immune parameters of fish were determined. Results showed that compared with control, the survival rates of the treatment groups were significantly increased ( p < .05 ). Lysozyme, component 3, component 4, immunoglobulin M, acid phosphatase, and alkaline phosphatase levels in serum by dietary106-109 CFU/g B. velezensis showed significant promoting effects ( p < .05 ). In conclusion, dietary 107-109 CFU/g B. velezensis boosts the growth, immunity, antioxidant capacity, and against A. hydrophila infection of Amur minnow. Regression analysis suggested that the optimal dose of B. velezensis LSG2-5 in the Amur minnow diet was 5.00 × 10 8 CFU/g.

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“…Fermented drinks derived from cereals and legumes can aid in the production of a better functional beverage ( Das et al, 2016 ; Panda et al, 2018 ). Another study was conducted to isolate and characterize the effects of traditionally prepared probiotic-containing functional fermented rice beer of Assam, where it was found that the isolates were gamma-hemolytic in nature and the cell-free supernatant concentrate displayed no toxicity against mouse liver cells ( Borah et al, 2019 ). In another study, Ghosh et al (2015) used the probiotic strain Lb.…”

Section: Rice Beverage As a Source Of Pro- And Prebioticsmentioning

confidence: 99%

Synbiotic Effects of Fermented Rice on Human Health and Wellness: A Natural Beverage That Boosts Immunity

Fuloria

¹

,

Mehta

²

,

Talukdar

³

et al. 2022

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Fermented foods have been an important component of the human diet from the time immemorial. It contains a high amount of probiotics that have been associated to a wide range of health benefits, including improved digestion and immunity. This review focuses on the indigenously prepared prebiotic- and probiotic-containing functional fermented rice (named Xaj-pani) by the Ahom Community from Assam, in Northeast India, including all the beneficial and potential effects on human health. Literature was searched from scientific databases such as PubMed, ScienceDirect and Google Scholar. Glutinous rice (commonly known as bora rice of sali variety) is primarily employed to prepare beverages that are recovered through the filtration process. The beer is normally consumed during religious rites, festivals and ritual practices, as well as being used as a refreshing healthy drink. Traditionally, it is prepared by incorporating a variety of medicinal herbs into their starter culture (Xaj-pitha) inoculum which is rich in yeasts, molds and lactic acid bacteria (LAB) and then incorporated in alcoholic beverage fermentation. The Ahom communities routinely consume this traditionally prepared alcoholic drink with no understanding of its quality and shelf life. Additionally, a finally produced dried cake, known as vekur pitha act as a source of Saccharomyces cerevisiae and can be stored for future use. Despite the rampant use in this community, the relationship between Xaj-pani’s consumption, immunological response, infectious and inflammatory processes remains unknown in the presence of factors unrelated or indirectly connected to immune function. Overall, this review provides the guidelines to promote the development of prebiotic- and probiotic-containing functional fermented rice that could significantly have an impact on the health of the consumers.

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Probiotic characterization of indigenous Bacillus velezensis strain DU14 isolated from Apong, a traditionally fermented rice beer of Assam

Cited by 31 publications

References 28 publications

Impact of Sinorhizobium meliloti Exopolysaccharide on Adsorption and Aggregation in the Copper(II) Ions/Supporting Electrolyte/Kaolinite System

Impact of Sinorhizobium meliloti Exopolysaccharide on Adsorption and Aggregation in the Copper(II) Ions/Supporting Electrolyte/Kaolinite System

Effects of Dietary Bacillus velezensis LSG2-5 on Growth, Immunity, Antioxidant Capacity, and Disease Resistance of Amur minnow (Rhynchocypris lagowskii Dybowski)

Synbiotic Effects of Fermented Rice on Human Health and Wellness: A Natural Beverage That Boosts Immunity

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