Probiotic characterization of Bacillus subtilis P223 isolated from kimchi

Jeon, Hyelin; Lee, Na‐Kyoung; Yang, Seung Won; Kim, Won-Suck; Paik, Hyun‐Dong

doi:10.1007/s10068-017-0148-5

Cited by 60 publications

(39 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…was beta-hemolytic in our assays. Similarly, Bacillus clausii isolates had been previously reported as non-hemolytic (Funke et al 1997;Jeon et al 2017), whereas in our assays, Bacillus spp. isolates N23 and N24 possess beta-hemolytic activity.…”

Section: Discussionsupporting

confidence: 61%

“…The distinction between α and β hemolysis is of relevance in some areas, such as the assessment of the safety of probiotics (Jeon et al 2017), but it is not generally made in other settings because both types of hemolysis are usually considered a virulence determinant and one of the many phenotypes studied to determine the clinical relevance of the species and/or strains (Bayley 1997;Ramachandran 2013). For this reason, in this study, isolates with either α-hemolytic or β-hemolytic activity are referred to as hemolytic.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Potentially pathogenic bacteria isolated from diverse habitats in Spitsbergen, Svalbard

2020

View full text Add to dashboard Cite

The Arctic ecosystem, a reservoir of genetic microbial diversity, represents a virtually unlimited source of microorganisms that could interact with human beings. Despite continuous exploration of Arctic habitats and description of their microbial communities, bacterial phenotypes commonly associated with pathogenicity, such as hemolytic activity, have rarely been reported. In this study, samples of snow, fresh and marine water, soil, and sediment from several habitats in the Arctic archipelago of Svalbard were collected during Summer, 2017. Bacterial isolates were obtained after incubation on oligotrophic media at different temperatures and their hemolytic potential was assessed on sheep blood agar plates. Partial (α) or true (β) hemolysis was observed in 32 out of 78 bacterial species. Genes expressing cytolytic compounds, such as hemolysins, likely increase the general fitness of the producing microorganisms and confer a competitive advantage over the availability of nutrients in natural habitats. In environmental species, the nutrient-acquisition function of these compounds presumably precedes their function as toxins for mammalian erythrocytes. However, in the light of global warming, the presence of hemolytic bacteria in Arctic environments highlights the possible risks associated with these microorganisms in the event of habitat melting/destruction, ecosystem transition, and re-colonization.

show abstract

Section: Discussionsupporting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

Potentially pathogenic bacteria isolated from diverse habitats in Spitsbergen, Svalbard

2020

View full text Add to dashboard Cite

show abstract

“…L. plantarum 200655 did not produce b-glucuronidase, alkaline phosphate, esterase, esterase lipase, lipase, cystine arylamidase, trypsin, a-chymotrypsin, acid phosphatase, a-mannosidase, and a-fucosidase. Because b-glucuronidase is a bacterial carcinogenic enzyme that exerts negative effects in the liver, LAB should not produce b-glucuronidase (Jeon et al, 2017). The potential probiotic L. brevis FI10700 and L. perolens FI10842 did not produce b-glucuronidase (Son et al, 2017).…”

Section: Enzyme Production Of L Plantarum 200655mentioning

confidence: 99%

“…Kimchi is a famous traditional vegetable food in Korea that is fermented by lactic acid bacteria (LAB) at low temperature (Jeon et al, 2017). During the storage period, LAB produce the unique flavor of kimchi and compounds beneficial for health by changing the composition of kimchi through fermentation (Lee et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and immune-enhancing effects of probiotic Lactobacillus plantarum 200655 isolated from kimchi

Yang

Lee

Lim

et al. 2018

Food Sci Biotechnol

Self Cite

View full text Add to dashboard Cite

Probiotic properties including antioxidant and immune-enhancing effects of Lactobacillus plantarum 200655 isolated from kimchi were evaluated. The tolerance of three strains (L. plantarum 200655, L. plantarum KCTC 3108, and L. rhamnosus GG to bile salts (0.3% oxgall, 24 h) was similar, and L. plantarum 200655 showed the highest tolerance to gastric juice (0.3% pepsin, 3 h). All strains presented similar autoaggregation ability. L. plantarum 200655 showed higher cell surface hydrophobicity and adhesion ability on HT-29 cells. L. plantarum 200655 did not produce b-glucuronidase and was sensitive to ampicillin, tetracycline, chloramphenicol, and doxycycline. Additionally, L. plantarum 200655 showed the highest antioxidant effects in DPPH and ABTS radical scavenging, and b-carotene bleaching assays. RAW 264.7 cells treated with L. plantarum 200655 produced more nitric oxide, induced nitric oxide synthase, and cytokine related to immune-enhancing effects such as interleukin-1b and interleukin-6. Therefore, L. plantarum 200655 could be useful as a probiotic strain for older people.

show abstract

“…Autoaggregation and coaggregation assays were modified slightly from Jeon et al (2017). The overnight culture of bacterial cells was harvested by centrifugation at 6,000×g for 10 min at 4℃.…”

Section: Autoaggregation and Coaggregation Propertiesmentioning

confidence: 99%

Probiotic Properties of Lactobacillus brevis KU200019 and Synergistic Activity with Fructooligosaccharides in Antagonistic Activity against Foodborne Pathogens

Kariyawasam¹,

Yang²,

Lee³

et al. 2020

Food Sci Anim Resour

Self Cite

View full text Add to dashboard Cite

This study aims to evaluate the probiotic properties of Lactobacillus brevis (L. brevis) KU200019 and the synergistic activity with prebiotics on antimicrobial activity, and the potential application as an adjunct culture in fermented dairy products. The commercial strain, L. brevis ATCC 14869 was used as reference strain. L. brevis KU200019 was showed higher viability in simulated gastric (99.38±0.21%) and bile (115.10±0.13%) conditions compared to reference strain. L. brevis KU200019 exhibited antimicrobial activity against various foodborne pathogens. The supplementation of fructooligosaccharides (FOS) enhanced viability of lactic acid bacteria (>8 Log CFU/mL) and antioxidant activity [2,2diphenyl-2-picrylhydrazyl radical assay (DPPH) assay, 31.23±1.14%; 2,2-azinobis (3ethylbenzothiazoline-6-sulfonic acid (ABTS) assay, 38.82±1.46%] in fermented skim milk during refrigerated storage. L. brevis KU200019 was distinguished from the reference strain by its higher probiotic potential, antimicrobial activity, and higher antioxidant activity in fermented milk. Therefore, L. brevis KU200019 with FOS was demonstrated promising properties for further application in fermented dairy products with enhanced safety and quality.

show abstract

Probiotic characterization of Bacillus subtilis P223 isolated from kimchi

Cited by 60 publications

References 26 publications

Potentially pathogenic bacteria isolated from diverse habitats in Spitsbergen, Svalbard

Potentially pathogenic bacteria isolated from diverse habitats in Spitsbergen, Svalbard

Antioxidant and immune-enhancing effects of probiotic Lactobacillus plantarum 200655 isolated from kimchi

Probiotic Properties of Lactobacillus brevis KU200019 and Synergistic Activity with Fructooligosaccharides in Antagonistic Activity against Foodborne Pathogens

Contact Info

Product

Resources

About