Probiotic characterization and cholesterol assimilation ability of Pichia kudriavzevii isolated from the gut of the edible freshwater snail “Pila globosa”.

Kathade, Suyash Arunrao; Aswani, Mayur Arjun; Anand, Pashmin Kaur; Nirichan, Bipinraj

doi:10.21608/ejabf.2020.119039

Cited by 13 publications

(9 citation statements)

References 19 publications

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“…LC528142.1), and Saccharomyces boulardii (SB) were procured from Pathology lab, Rajiv Gandhi Institute of I.T. and Biotechnology, Bharati Vidyapeeth (Deemed to be University), Pune [23][24][25][26] .…”

Section: Materials and Methods Sample Collectionmentioning

confidence: 99%

Prebiotic Profiling of Indigenous Selected Dioscorea Spp. Using In-vitro Techniques

Aswani

Kathade

Nisar

et al. 2022

Biosci., Biotech. Res. Asia

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The current study used an in-vitro technique to evaluate the functional potential of Dioscorea alata L. and D. bulbifera L. extracts as prebiotics. Prebiotics are nondigestible carbohydrates that undergo a selective fermentation process in the gut to benefit the host, according to Gibson and Roberfroid in 1995. Many wild edible plants are high in carbohydrates and are utilised as both a staple food and medicine for a variety of stomach-related disorders. This study employed sweet tuber (ST), bitter tuber (BT), sweet bulbils (SB), and bitter bulbils (BB) from D. bulbifera, as well as tuber (AT) from D. alata and extracted prebiotics using standard method.The AT plant sample seemed to have the least reducing sugars, with a concentration of 2.83 mg/mL. The prebiotic activity of ST, BT, SB, BB, and AT samples was examined as the sole carbon source for microorganisms; among these, AT exhibited a considerable increase in the growth of recognised probiotics Lactobacillus plantarum, Saccharomyces cerevisiae, S. boulardii, and Pichia spp. in-vitro when compared to fructooligosaccharides (FOS). This preliminary investigation indicates that AT has the potential to be used as a promising prebiotic.

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Section: Materials and Methods Sample Collectionmentioning

confidence: 99%

Prebiotic Profiling of Indigenous Selected Dioscorea Spp. Using In-vitro Techniques

Aswani

Kathade

Nisar

et al. 2022

Biosci., Biotech. Res. Asia

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show abstract

“…The suspension was vortexed for 2 min and incubated at 37ºC for phase separation. The decrease in absorbances of the aqueous phase was measured as per cent hydrophobicity (H %) and calculated as H %=[(A0-A)/A0]*100, where A0 and A are the absorbances of the culture in the aqueous phase before and after extraction respectively (18).…”

Section: Hydrophobicity Assaymentioning

confidence: 99%

Isolation, Characterization, and Diversity of Probiotic Microorganisms from Different Postpartum Milk of Various Animals

Kathade

Aswani

Anand

2022

Int J Health Sci Res

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Most importantly milk contains many beneficial probiotic microorganisms, hence, it has been sourced for the isolation of many probiotic bacteria. Due to the huge microbial diversity and functionality, there is scope to isolate microorganisms, evaluate them for probiotic potential. It is also important to compare the diversity of probiotic microorganisms in the postpartum periods of milk. In the present study, cultures were isolated from three different postpartum periods milk of Goat, Sheep, Cow, and Buffalo. The 182 cultures were isolated, only 127 were selected by colony characterization and 69 were selected after biochemical analysis such as Gram nature, endospore, catalase, acid-fast, and mannitol fermentation, 54 were non-hemolytic, 47 could tolerate a wide range of pH, bile, temperature, and NaCl. 30 isolates could survive in the presence of a gastric and intestinal environment. These cultures showed inhibitory activity against enteropathogens and did satisfy the need for auto and co-aggregation property. 17 cultures showed BSH and hydrophobicity activity, only 6 cultures showed susceptibility against all types of antibiotics. As per the results from the total potential probiotic cultures from various animal milk samples, 80% from first and second postpartum and 20% cultures from third postpartum milk were isolated, as per the results as milk ages, the probiotic concentration in the milk decreases. Key words: Postpartum, Milk, Probiotics, Yeast, Lactobacillus.

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“…In recent years, due to the food habits, people are suffering more with several cardiovascular and cerebrovascular diseases [7] associated with high cholesterol concentration. This also includes heart stroke, peripheral artery disease, diabetes, and high blood pressure [8]. Therefore, the cholesterol-lowering ability of probiotics and the associated mechanisms for cholesterol removal have received a great attention.…”

Section: Introductionmentioning

confidence: 99%

“…Reports have shown that consumption of these probiotics supplemented food can help in cholesterol reduction by producing bile salt hydrolase (BSH) enzyme and therefore, production of BSH has become a crucial selection criterion for probiotic organisms to determine their cholesterol removal ability. Besides producing BSH, probiotics also possess some inherent ability to scavenge cholesterol [8]. With reference to this, we have also screened out our isolates on the basis of BSH production and assessed their potential for cholesterol removal.…”

Section: Introductionmentioning

confidence: 99%

In-vitro investigation of cholesterol removal, ß-galactosidase synthesis, antioxidant, and antidiabetic potential of probiotic organisms

Chanpura¹,

Gupte²

2022

J App Biol biotech

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The present study is aimed to determine some important health beneficial properties of probiotic isolates such as cholesterol removal, β-galactosidase production, antioxidant, and anti-diabetic activity. Nine probiotic isolates were screened for Bile Salt Hydrolase (BSH) activity and potential BSH producers were selected for cholesterol removal study. Isolate B11 has shown significant cholesterol removal ability (88 ± 0.2%) after 24 h and this property was also analyzed with variable concentrations, time profile, with live, and dead cells as well as from egg yolk with simulation treatment. As probiotics improve lactose intolerance, all isolates were assessed for their lactose utilizing and β-galactosidase production ability where maximum enzyme activity was observed in case of isolate D25 (226 ± 0.30 Miller units). All the isolates were also assessed for their antioxidant potential using four different methods such as reducing power assay, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, 2,2-diphenyl-1-picrylhydrazyl assay, and superoxide dismutase activity. Out of nine isolates, isolate D25 and B11 have shown significant antioxidant property. Further, these isolates were also subjected to anti-diabetic study, where isolate D25 (89 ± 0.01%) and B11 (98 ± 0.30%) have shown high α-glucosidase inhibition which indicate their effective anti-diabetic activity. Hence, overall probiotic isolate D25 and B11 have shown significant health beneficial properties and they were further identified as Lactiplantibacillus plantarum and Pediococcus pentosaceus, respectively.

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Probiotic characterization and cholesterol assimilation ability of Pichia kudriavzevii isolated from the gut of the edible freshwater snail “Pila globosa”.

Cited by 13 publications

References 19 publications

Prebiotic Profiling of Indigenous Selected Dioscorea Spp. Using In-vitro Techniques

Prebiotic Profiling of Indigenous Selected Dioscorea Spp. Using In-vitro Techniques

Isolation, Characterization, and Diversity of Probiotic Microorganisms from Different Postpartum Milk of Various Animals

In-vitro investigation of cholesterol removal, ß-galactosidase synthesis, antioxidant, and antidiabetic potential of probiotic organisms

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