Probiotic characteristics and whole-genome sequence analysis of Pediococcus acidilactici isolated from the feces of adult beagles

Abstract

The beneficial effects of lactic acid bacteria are well known and recognized as functional foods that are health benefits for companion animals. This study, for the first time, reports the probiotic properties, safety, and whole-genome sequence of Pediococcus acidilactici GLP06 isolated from feces of beagles. In this study, candidate probiotic bacteria P. acidilactici GLP02 and GLP06 were morphologically characterized and tested for their antimicrobial capacity, tolerance to different conditions (low pH, bile salts, an artificial gastrointestinal model, and high temperature), antibiotic sensitivity, hemolytic activity, cell surface hydrophobicity, autoaggregation activity, and adhesion to Caco-2 cells. P. acidilactici GLP06 showed better probiotic potential. Therefore, P. acidilactici GLP06 was evaluated for in vivo safety in mice and whole-genome sequencing. The results showed, that the supplemented MG06 group (1010 cfu/mL), GLP06 was not only nontoxic to mice, but also promoted the development of the immune system, improved resistance to oxidative stress, and increased the diversity of intestinal microorganisms and the abundance of Lactobacillus. Whole-genome sequencing showed that P. acidilactici GLP06 was 2,014,515 bp and contained 1,976 coding sequences, accounting for 86.12% of the genome, with no drug resistance genes and eight CRISPR sequences. In conclusion, the newly isolated canine-derived P. acidilactici GLP06 had good probiotic potential, was nontoxic to mice and promoted the development of immune organs, improved the biodiversity of the intestinal flora, and had no risk of drug-resistant gene transfer, indicating that P. acidilactici GLP06 can be used as a potential probiotic for the prevention and treatment of gastrointestinal diseases in companion animals.

Impact of Pediococcus acidilactici GLP06 supplementation on gut microbes and metabolites in adult beagles: a comparative analysis

“…Previous studies have demonstrated that P. acidilactici can improve host gut microbiota, stimulate various non-specific immunities, and inhibit the growth of pathogenic bacteria in the gut intestinal tract ( Ferguson et al, 2010 ; Fernandez et al, 2016 ; Kim et al, 2018 ; Zhang et al, 2022 ). Regarding P. acidilactici GLP06, our previous study has shown that canine-derived P. acidilactici GLP06 has probiotic potential and antioxidant capacity and is safe and free of drug-resistance genes ( Zhao et al, 2023 ). In this study, neither ATTD nor nitrogen metabolism was significantly altered in beagles fed with P. acidilactici GLP06.…”

“…P. acidilactici GLP06, used in this study, was isolated from the gastrointestinal tract of healthy beagles and completed the probiotic potential and safety evaluation in our laboratory ( Zhao et al, 2023 ). It was kept in the China Centre for Type Culture Collection (CCTCC; Wuhan, China) under the accession number CCTCC No.…”

Acetolactate Decarboxylase as an Important Regulator of Intracellular Acidification, Morphological Features, and Antagonism Properties in the Probiotic Lactobacillus reuteri

“…Antibiotic susceptibility was classified as sensitive (≥ 21 mm), intermediate (16-20 mm), or resistant (≤15 mm). [52]…”

Evaluation of Probiotic Properties and Safety of Lactobacillus helveticus LH10 Derived from Vinegar through Comprehensive Analysis of Genotype and Phenotype

“…Currently, the integration of whole-genome sequencing and bioinformatics could comprehensively explore the genetic and biological characteristics of strains with enhanced resolution and sensitivity. This approach provides valuable insights into various aspects such as genetic information, evolutionary relationships, physiological traits, probiotic potential, safety profiles, among others [14]. However, it is crucial to acknowledge that phenotypic analysis remains indispensable for probiotic discovery due to potential errors in the sequencing process and limited concordance between genomic sequences and existing databases [15].…”