Probiotic biomarkers and models upside down: From humans to animals

Trukhachev, Vladimir; Chmykhalo, Victor; Belanova, Anna; Beseda, Darya K.; Chikindas, Michael L.; Bren, Anzhelica B.; Ermakov, Alexey; Донник, И. М.; Belousova, Marya M.; Zolotukhin, Peter

doi:10.1016/j.vetmic.2021.109156

Cited by 14 publications

(10 citation statements)

References 47 publications

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“…Different methods and models have been used to assess the adhesion of probiotics. HT-29 cell lines are the main cell models for adhesion ability assessments, both within and outside of China because these cell lines consist of mature intestinal epithelial cells ( Trukhachev et al, 2021 ). However, bacterial persistence is easily antagonized by other strains in the intestinal microbiota.…”

Section: Introductionmentioning

confidence: 99%

Spatial and Temporal Persistence of Fluorescent Lactiplantibacillus plantarum RS-09 in Intestinal Tract

Zhao

Yang

et al. 2022

Front. Microbiol.

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The beneficial effects of the probiotic strain Lactiplantibacillus plantarum (formerly Lactobacillus plantarum) are based on its adherence and colonization ability in the gut. However, little is known about the migration and long-term gut colonization of the strain. This study evaluated the gut colonization modes of Lactiplantibacillus plantarum RS-09 to identify the strain with long-term gut colonization potential. We established CFDA/SE-labeled RS-09 to study the temporal and spatial distribution of RS-09 in the intestine as well as to analyze its persistence in different parts of the intestine by flow cytometry. This study has shown that the RS-09 strain maintains strong adhesion abilities under acid (pH 2.5) and base (pH 8.5) conditions. In addition, CFDA/SE can be used as an indicator for the labeling of L. plantarum RS-09 in the intestinal tract in vivo. We established a growth kinetics model of RS-09 to elucidate its persistence in the intestine. In vivo persistence experiments showed that the persistence rate of RS-09 was the highest in the cecum (69.5%) and the lowest in the duodenum (12.8%) at 96 h. After 20 days, RS-09 was predominantly localized in the cecum and colon steadily. These studies provide new insights into the long-term persistence of L. plantarum in the gastrointestinal tract. The CFDA/SE label system may be used to study the in vivo colonization dynamics of other probiotic strains.

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Section: Introductionmentioning

confidence: 99%

Spatial and Temporal Persistence of Fluorescent Lactiplantibacillus plantarum RS-09 in Intestinal Tract

Zhao

Yang

et al. 2022

Front. Microbiol.

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“…Generally, aminoglycosides resistance is prevalent in lactic acid bacteria (LAB), where the main concern is the transferability of genetic determinants [ 70 , 71 ]. Regarding the cytotoxicity/anti-proliferative activity of current probiotics, Caco-2 and HT-29 are the most commonly used cell lines [ 72 ]. In the present study, the two E. lactis strains’ CFSs showed a non-significant difference from the control ( P > 0.05) overall.…”

Section: Discussionmentioning

confidence: 99%

Safety assessment of Enterococcus lactis strains complemented with comparative genomics analysis reveals probiotic and safety characteristics of the entire species

Ahmed,

Khattab,

Ragab

et al. 2023

BMC Genomics

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Background The gut microbiota is considered a rich source for potential novel probiotics. Enterococcus genus is a normal component of a healthy gut microbiota, suggesting its vital role. Nosocomial infections caused mainly by E. facalis and E. faecium have been attributed to the plasticity of the Enterococcus genomes. In this study, we assessed the probiotic and safety characteristics of two E. lactis strains isolated from the human gut microbiota using in-vitro and in silico approaches. Additionally, the safety of the E. lactis species was evaluated using comparative genomics analysis. Results The two E. lactis strains 10NA and 50NA showed resistance to bile salts and acid tolerance with antibacterial activity against Escherichia coli, Salmonella typhi, and Clostridioides difficile. For safety assays, the two strains did not display any type of hemolysis on blood agar, and the survival of Caco-2 cells was not significantly different (P-value > 0.05) compared to the control using cell free supernatants at 100% (v/v), 50% (v/v), 10% (v/v), and 5% (v/v) concentrations. Regarding antibiotic susceptibility, both strains were sensitive to vancomycin, tetracycline, and chloramphenicol. Comprehensive whole-genome analysis revealed no concerning associations between virulence or antibiotic resistance genes and any of the identified mobile genetic elements. Comparative genome analysis with closely related E. faecium species genomes revealed the distinctive genomic safety of the E. lactis species. Conclusions Our two E. lactis strains showed promising probiotic properties in-vitro. Their genomes were devoid of any transferable antibiotic resistance genes. In silico comparative analysis confirmed the safety of the E. lactis species. These results suggest that E. lactis species could be a potential source for safer Enterococcus probiotic supplements.

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“…Butyrate-producing probiotic strains (e.g., Butyricicoccus pullicaecorum ), improved feed efficiency and lowered Campylobacter levels in the ceca, by ∼1.5 logs, when fed to Ross 308 chicken broilers (Eeckhaut et al, 2016 ). Probiotic-derived butyrate also reduces the expression of proinflammatory cytokines (interleukin [IL]-6, IL-10, IL-1β, and IFN-γ) and hinders the production of nitric oxide in lipopolysaccharide (LPS)-stimulated cells (Trukhachev et al, 2021 ). Likewise, birds subjected to an oral gavage with a probiotic suspension of Lactobacillus salivarius SMXD51, 10 7 CFU at 14 and 35 d, showed significant reductions (0.82–2.81 logs) in Campylobacter levels (Saint-Cyr et al, 2017 ).…”

Section: Probiotics and Campylobacter Colonization...mentioning

confidence: 99%

“…Probiotics can create a molecular weaponry necessary to outcompete and eliminate pathogens, colonize the environmental niche, and secrete various antimicrobial components. They can deploy enzymatic mechanisms responsible for modifying pathogen toxin receptors, hydrolysis of bacterial toxins, and inhibition of pathogen-induced illness (Trukhachev et al, 2021 ).…”

Section: Probiotics and Preclinical Outcomes In Human Campy...mentioning

confidence: 99%

Anti-Campylobacter Probiotics: Latest Mechanistic Insights

Balta

Butucel

Ştef

et al. 2022

Foodborne Pathogens and Disease

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The Campylobacter genus is the leading cause of human gastroenteritis, with the consumption of contaminated poultry meat as the main route of infection. Probiotic bacteria, such as Lactobacillus , Bacillus , Escherichia coli Nissle, and Bifidobacterium species, have a great immunomodulatory capacity and exhibit antipathogenic effects through various molecular mechanisms. Reducing Campylobacter levels in livestock animals, such as poultry, will have a substantial benefit to humans as it will reduce disease transmissibility through the food chain. Moreover, probiotic-based strategies might attenuate intestinal inflammatory processes, which consequently reduce the severity of Campylobacter disease progression. At a molecular level, probiotics can also negatively impact on the functionality of various Campylobacter virulence and survival factors (e.g., adhesion, invasion), and on the associated colonization proteins involved in epithelial translocation. The current review describes recent in vitro , in vivo , and preclinical findings on probiotic therapies, aiming to reduce Campylobacter counts in poultry and reduce the pathogen's virulence in the avian and human host. Moreover, we focused in particular on probiotics with known anti- Campylobacter activity seeking to understand the biological mechanisms involved in their mode of action.

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Probiotic biomarkers and models upside down: From humans to animals

Cited by 14 publications

References 47 publications

Spatial and Temporal Persistence of Fluorescent Lactiplantibacillus plantarum RS-09 in Intestinal Tract

Spatial and Temporal Persistence of Fluorescent Lactiplantibacillus plantarum RS-09 in Intestinal Tract

Safety assessment of Enterococcus lactis strains complemented with comparative genomics analysis reveals probiotic and safety characteristics of the entire species

Anti-Campylobacter Probiotics: Latest Mechanistic Insights

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