Probiotic bacteria inhibit the bovine respiratory pathogen<i>Mannheimia haemolytica</i>serotype 1<i>in vitro</i>

Verdú, Samuel; Subramanian, Senthil Kumar; Timsit, Edouard; Alexander, Trevor W.

doi:10.1111/lam.12723

Cited by 23 publications

(17 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The BT cocktail reduced nasal colonization by M. haemolytica, which was expected based on our previous in vitro studies (19,22). Indeed, all six Lactobacillus strains inoculated have been shown to inhibit M. haemolytica adherence to bovine turbinate cells by 32% to 78% (22).…”

Section: Discussionsupporting

confidence: 73%

“…The fact that Lactococcus could antagonize M. haemolytica is in agreement with a previous study (21) which showed that Lactococcus was more abundant in the lower respiratory tract of healthy feedlot cattle than cattle with BRD (which had a higher abundance of M. haemolytica). Furthermore, we have previously shown that Lactococcus lactis could inhibit the growth of M. haemolytica in vitro (19). Lactococcus spp.…”

Section: Discussionmentioning

confidence: 96%

“…Increasing evidence indicates that commensal bacteria in the bovine nasopharynx may prevent the colonization and proliferation of bacterial pathogens through a variety of mechanisms, including direct antagonism (i.e., antimicrobial properties), competition for nutrients and adhesion sites, and host immunomodulatory effects (15)(16)(17)(18). Among commensal bacteria, lactic acid-producing bacteria (LAB), and more specifically Lactobacillus spp., may be important in providing colonization resistance against bacterial respiratory pathogens (19)(20)(21). In a previous study, we used a targeted approach to characterize bacterial therapeutic (BT) candidates originating from the bovine nasopharynx (22).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Intranasal Bacterial Therapeutics Reduce Colonization by the Respiratory Pathogen Mannheimia haemolytica in Dairy Calves

et al. 2020

View full text Add to dashboard Cite

Six Lactobacillus strains originating from the nasopharyngeal microbiota of cattle were previously characterized in vitro and identified as candidate bacterial therapeutics (BTs) for mitigating the bovine respiratory pathogen Mannheimia haemolytica. In the present study, these BT strains were evaluated for their potential to (i) reduce nasal colonization by M. haemolytica, (ii) modulate the nasal microbiota, and (iii) stimulate an immune response in calves experimentally challenged with M. haemolytica. Twenty-four Holstein bull calves (1 to 3 weeks old) received either an intranasal BT cocktail containing 6 Lactobacillus strains (3 × 109 CFU per strain; BT + Mh group) 24 h prior to intranasal M. haemolytica challenge (3 × 108 CFU) or no BTs prior to challenge (Mh, control group). Nasal swab, blood, and transtracheal aspiration samples were collected over the course of 16 days after BT inoculation. Counts of M. haemolytica were determined by culturing, and the nasal and tracheal microbiotas were evaluated using 16S rRNA gene sequencing. Serum cytokines (interleukin-6 [IL-6], IL-8, and IL-10) were quantified by enzyme-linked immunosorbent assay (ELISA). Administration of BT reduced nasal colonization by M. haemolytica (P = 0.02), modified the composition and diversity of the nasal microbiota, and altered interbacterial relationships among the 10 most relatively abundant genera. The BT + Mh calves also had a lower relative abundance of Mannheimia in the trachea (P < 0.01) but similar cytokine levels as Mh calves. This study demonstrated that intranasal BTs developed from the bovine nasopharyngeal Lactobacillus spp. were effective in reducing nasal colonization by M. haemolytica in dairy calves. IMPORTANCE Bovine respiratory disease (BRD) is one of the significant challenges for the modern dairy industry in North America, accounting for 23 to 47% of the total mortality among pre- and postweaned dairy heifers. Mass medication with antibiotics is a common practice to control BRD in dairy cattle. However, the emergence of multidrug-resistant BRD pathogens highlights the importance of developing alternatives to antibiotics for BRD mitigation. Using a targeted approach, we recently identified 6 Lactobacillus strains originating from the bovine respiratory microbiota as candidates to be used as bacterial therapeutics (BTs) for the mitigation of the BRD pathogen Mannheimia haemolytica. Here, we demonstrated that intranasal inoculation of the BT strains reduced nasal colonization by M. haemolytica in dairy calves experimentally challenged with this pathogen. This study, for the first time, shows the potential use of intranasal BTs as an alternative to mitigate BRD pathogens in cattle.

show abstract

Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 96%

mentioning

confidence: 99%

See 1 more Smart Citation

Intranasal Bacterial Therapeutics Reduce Colonization by the Respiratory Pathogen Mannheimia haemolytica in Dairy Calves

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Recent studies have also suggested an association between the nasopharyngeal microbiota and development of BRD in feedlot cattle (15,16). This notion is further supported by studies associating a greater relative abundance of nasopharyngeal Lactobacillaceae at the time of feedlot entry with protection against BRD (17) and also specific inhibition of M. haemolytica in vitro (18). Hence, maintaining a stable microbial community in the nasopharynxes of cattle after feedlot placement may offer protection against BRD development, and bacteria colonizing the bovine respiratory tract may have potential for use as therapeutics to mitigate BRD pathogens.…”

mentioning

confidence: 79%

Development of Bacterial Therapeutics against the Bovine Respiratory Pathogen Mannheimia haemolytica

Amat

Timsit

Baines

et al. 2019

Appl Environ Microbiol

View full text Add to dashboard Cite

Bovine respiratory disease (BRD) is a major cause of morbidity and mortality in beef cattle. Recent evidence suggests that commensal bacteria of the bovine nasopharynx have an important role in maintaining respiratory health by providing colonization resistance against pathogens. The objective of this study was to screen and select bacterial therapeutic candidates from the nasopharynxes of feedlot cattle to mitigate the BRD pathogen Mannheimia haemolytica. In a stepwise approach, bacteria (n = 300) isolated from the nasopharynxes of 100 healthy feedlot cattle were identified and initially screened (n = 178 isolates from 12 different genera) for growth inhibition of M. haemolytica. Subsequently, selected isolates were evaluated for the ability to adhere to bovine turbinate (BT) cells (n = 47), compete against M. haemolytica for BT cell adherence (n = 15), and modulate gene expression in BT cells (n = 10). Lactobacillus strains had the strongest inhibition of M. haemolytica, with 88% of the isolates (n =33) having inhibition zones ranging from 17 to 23 mm. Adherence to BT cells ranged from 3.4 to 8.0 log10 CFU per 105 BT cells. All the isolates tested in competition assays reduced M. haemolytica adherence to BT cells (32% to 78%). Among 84 bovine genes evaluated, selected isolates upregulated expression of interleukin 8 (IL-8) and IL-6 (P < 0.05). After ranking isolates for greatest inhibition, adhesion, competition, and immunomodulation properties, 6 Lactobacillus strains from 4 different species were selected as the best candidates for further development as intranasal bacterial therapeutics to mitigate M. haemolytica infection in feedlot cattle. IMPORTANCE Bovine respiratory disease (BRD) is a significant animal health issue impacting the beef industry. Current BRD prevention strategies rely mainly on metaphylactic use of antimicrobials when cattle enter feedlots. However, a recent increase in BRD-associated bacterial pathogens that are resistant to metaphylactic antimicrobials highlights a pressing need for the development of novel mitigation strategies. Based upon previous research showing the importance of respiratory commensal bacteria in protecting against bronchopneumonia, this study aimed to develop bacterial therapeutics that could be used to mitigate the BRD pathogen Mannheimia haemolytica. Bacteria isolated from the respiratory tracts of healthy cattle were characterized for their inhibitory, adhesive, and immunomodulatory properties. In total, 6 strains were identified as having the best properties for use as intranasal therapeutics to inhibit M. haemolytica. If successful in vivo, these strains offer an alternative to metaphylactic antimicrobial use in feedlot cattle for mitigating BRD.

show abstract

“…A recent study revealed that the relative abundance of nasopharyngeal LAB in cattle entering the feedlot was significantly greater in animals that remained healthy compared with those that developed BRD [52], suggesting that a certain LAB are important to bovine respiratory mucosal health. Furthermore, an in vitro pilot study that we conducted to test antimicrobial properties of commercially available LAB strains (Lactobacillus) against M. haemolytica demonstrated the possibility of using probiotics to mitigate BRD pathogens [53]. The Lactobacillus strains isolated from the nasopharynx of healthy feedlot cattle displayed antimicrobial activity against the growth of M. haemolytica in vitro [54].…”

Section: Using Probiotics To Mitigate Brd Bacterial Pathogensmentioning

confidence: 99%

Bovine Respiratory Disease in Feedlot Cattle: Antimicrobial Resistance in Bovine Respiratory Bacterial Pathogens and Alternative Antimicrobial Approaches

Amat¹

2019

Bacterial Cattle Diseases

View full text Add to dashboard Cite

Bovine respiratory disease (BRD) is the leading cause of morbidity and mortality in feedlot cattle in North America. The BRD is a complex multifactorial disease because its onset depends on the interaction between number of factors including host, environment, management and viral and bacterial infectious agents. The main bacterial pathogens associated with BRD are Mannheimia haemolytica, Pasteurella multocida, Histophilus somni and Mycoplasma bovis. Treatment and prevention of BRD in the feedlots are aimed mainly at bacterial pathogens through antimicrobial use. Although antimicrobial use has increased, the prevalence of BRD has also increased potentially due to the emergence of multidrug-resistant bacterial pathogens, which poses a serious threat to both animal and public health and necessitates the development of alternative antimicrobial approaches to mitigate BRD pathogens in feedlot cattle. The objective of this chapter is to provide a brief overview of pathogenesis of BRD, to review the current status of antimicrobial resistance in bacterial pathogens associated with BRD, and to discuss the potential antimicrobial alternative strategies, including probiotic and essential oil (EO) approaches, to mitigate bovine respiratory pathogens in feedlot cattle.

show abstract

Probiotic bacteria inhibit the bovine respiratory pathogenMannheimia haemolyticaserotype 1in vitro

Cited by 23 publications

References 34 publications

Intranasal Bacterial Therapeutics Reduce Colonization by the Respiratory Pathogen Mannheimia haemolytica in Dairy Calves

Intranasal Bacterial Therapeutics Reduce Colonization by the Respiratory Pathogen Mannheimia haemolytica in Dairy Calves

Development of Bacterial Therapeutics against the Bovine Respiratory Pathogen Mannheimia haemolytica

Bovine Respiratory Disease in Feedlot Cattle: Antimicrobial Resistance in Bovine Respiratory Bacterial Pathogens and Alternative Antimicrobial Approaches

Contact Info

Product

Resources

About