Probiotic bacteria change Escherichia coli-induced gene expression in cultured colonocytes: Implications in intestinal pathophysiology

Pinaki,; Panigrahi,; Gheorghe,; Braileanu,; Hegang,; Chen, Guihai; Colin,; Stine,

doi:10.3748/wjg.13.6370

Cited by 13 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cultured colonocytes (Caco-2 cell line) incubated in the presence of a commensal Escherichia coli or L. plantarum resulted in different gene expression profiles (Panigrahi et al, 2007). Shima et al (2008) compared with gene expression from the intestinal epithelial cells of mice from different regions of the gastrointestinal tract after administration of Lactobacillus casei, Bifidobacterium breve, segmented filamentous bacteria, or a fecal suspension.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional profiling of cecal gene expression in probiotic- and Salmonella- challenged neonatal chicks

et al. 2011

View full text Add to dashboard Cite

Probiotics are currently used to improve health and reduce enteric pathogens in poultry. However, the mechanisms by which they reduce or prevent disease are not known. Salmonella are intracellular pathogens that cause acute gastroenteritis in humans, and infections by nontyphoid species of Salmonella also can result in diarrhea, dehydration, and depression in poultry. Frequently, however, no clinical signs of infection are apparent in poultry flocks. In this study, day-of-hatch chicks were challenged with Salmonella enterica serovar Enteritidis (SE) and treated 1 h later with a poultry-derived, Lactobacillus-based probiotic culture (FloraMax-B11, Pacific Vet Group USA Inc., Fayetteville, AR). Cecae were collected 12 and 24 h posttreatment for Salmonella detection and RNA isolation for microarray analysis of gene expression. At both 12 and 24 h, SE was significantly reduced in chicks treated with the probiotic as compared with the birds challenged with only SE (P < 0.05). Microarray analysis revealed gene expression differences among all treatment groups. At 12 h, 170 genes were expressed at significantly different levels (P < 0.05), with a minimum difference in expression of 1.2-fold. At 24 h, the number of differentially regulated genes with a minimum 1.2-fold change was 201. Pathway analysis revealed that at both time points, genes associated with the nuclear factor kappa B complex, as well as genes involved in apoptosis, were significantly regulated. Based on this analysis, probiotic-induced differential regulation of the genes growth arrest-specific 2 (GAS2) and cysteine-rich, angiogenic inducer, 61 (CYR61) may result in increased apoptosis in the cecae of chicks. Because Salmonella is an intracellular pathogen, we suggest that increased apoptosis may be a mechanism by which the probiotic culture reduces Salmonella infection.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcriptional profiling of cecal gene expression in probiotic- and Salmonella- challenged neonatal chicks

et al. 2011

View full text Add to dashboard Cite

show abstract

“…A previous study showed that L. plantarum CGMCC 1258 is able to protect against the disruption of four tight junction proteins caused by Enteroinvasive E. coli ATCC 43893 (serotype O124:NM) [17]. However, another study looking at the effect of L. plantarum ATCC202195 on the expression of genes in Caco-2 cells challenged with Enteroinvasive E. coli ATCC43893 (serotype O124:NM) did not report any changes in tight junction gene expression [31]. This suggests that the L. plantarum protection against tight junction disruption was not due to it altering host gene expression, and was likely due to it inhibiting the action of the pathogen in that study.…”

Section: Discussionmentioning

confidence: 99%

“…In addition to the study that showed that L. plantarum ATCC202195 nullifies changes in Caco-2 cell gene expression induced by Enteroinvasive E. coli ATCC43893 (serotype O124:NM) [31], other published studies investigating whole genome expression in response to L. plantarum have shown that, L. plantarum WCFSI induces increased expression of genes involved in lipid metabolism and cellular growth and development in healthy human duodenum [32] and L. plantarum (strain not given) alters the NF-κB pathway to limit inflammatory responses in healthy human duodenum [33]. However, in these published studies only a few tight junction-related genes had altered expression levels when exposed to L. plantarum , for example increased expression of the ZO-2 gene, so they are unlikely to contribute to changes in tight junction integrity, compared to the changes in 19 tight junction genes induced by L. plantarum MB452 reported in this study.…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus plantarum MB452 enhances the function of the intestinal barrier by increasing the expression levels of genes involved in tight junction formation

et al. 2010

View full text Add to dashboard Cite

BackgroundIntestinal barrier function is important for preserving health, as a compromised barrier allows antigen entry and can induce inflammatory diseases. Probiotic bacteria can play a role in enhancing intestinal barrier function; however, the mechanisms are not fully understood. Existing studies have focused on the ability of probiotics to prevent alterations to tight junctions in disease models, and have been restricted to a few tight junction bridging proteins. No studies have previously investigated the effect of probiotic bacteria on healthy intestinal epithelial cell genes involved in the whole tight junction signalling pathway, including those encoding for bridging, plaque and dual location tight junction proteins. Alteration of tight junction signalling in healthy humans is a potential mechanism that could lead to the strengthening of the intestinal barrier, resulting in limiting the ability of antigens to enter the body and potentially triggering undesirable immune responses.ResultsThe effect of Lactobacillus plantarum MB452 on tight junction integrity was determined by measuring trans-epithelial electrical resistance (TEER) across Caco-2 cell layers. L. plantarum MB452 caused a dose-dependent TEER increase across Caco-2 cell monolayers compared to control medium. Gene expression was compared in Caco-2 cells untreated or treated with L. plantarum MB452 for 10 hours. Caco-2 cell RNA was hybridised to human oligonucleotide arrays. Data was analysed using linear models and differently expressed genes were examined using pathway analysis tools. Nineteen tight junction-related genes had altered expression levels in response to L. plantarum MB452 (modified-P < 0.05, fold-change > 1.2), including those encoding occludin and its associated plaque proteins that anchor it to the cytoskeleton. L. plantarum MB452 also caused changes in tubulin and proteasome gene expression levels which may be linked to intestinal barrier function. Caco-2 tight junctions were visualised by fluorescent microscopy of immuno-stained occludin, zona occludens (ZO)-1, ZO-2 and cingulin. Caco-2 cells treated with L. plantarum MB452 had higher intensity fluorescence of each of the four tight junction proteins compared to untreated controls.ConclusionsThis research indicates that enhancing the expression of genes involved in tight junction signalling is a possible mechanism by which L. plantarum MB452 improves intestinal barrier function.

show abstract

“…2). Many data from different gastrointestinal disorders and conditions are based on results gained by using these cell lines representing the gastrointestinal tract 45–52…”

Section: Discussionmentioning

confidence: 99%

Inactivation of N‐TIMP‐1 by N‐terminal acetylation when expressed in bacteria

et al. 2008

View full text Add to dashboard Cite

The high-affinity binding of tissue inhibitors of metalloproteinases (TIMPs) to matrix metalloproteinases (MMPs) is essential for regulation of the turnover of the extracellular matrix during development, wound healing, and progression of inflammatory diseases such as cancer, atherosclerosis, and arthritis. Bacterially expressed N-terminal inhibitory domains of TIMPs (NTIMPs) have been used extensively for biochemical and biophysical study of interactions with MMPs. Titration of N-TIMP-1 expressed in E. coli indicates, however, that only about 42% of the protein is active as an MMP inhibitor. The separation of inactive from fully active N-TIMP-1 has been achieved both by MMP affinity and by high-resolution cation exchange chromatography at an appropriate pH, based on a slight difference of charge. Purification by cation exchange chromatography with a Mono S column enriches the active portion of N-TIMP-1 to > 95%, with K i of 1.5 nM for MMP-12. Mass spectra reveal that the inactive form differs from active N-TIMP-1 in being N-terminally acetylated, underscoring the importance of the free α-NH 2 of Cys1 for MMP inhibition. N α -acetylation of the CTCVPP sequence broadens the N-terminal sequence motifs reported to be susceptible to α-amino acetylation by E. coli N-acetyl transferases.

show abstract

Probiotic bacteria change Escherichia coli-induced gene expression in cultured colonocytes: Implications in intestinal pathophysiology

Cited by 13 publications

References 0 publications

Transcriptional profiling of cecal gene expression in probiotic- and Salmonella- challenged neonatal chicks

Transcriptional profiling of cecal gene expression in probiotic- and Salmonella- challenged neonatal chicks

Lactobacillus plantarum MB452 enhances the function of the intestinal barrier by increasing the expression levels of genes involved in tight junction formation

Inactivation of N‐TIMP‐1 by N‐terminal acetylation when expressed in bacteria

Contact Info

Product

Resources

About