Probiotic Bacillus subtilis 29,784 improved weight gain and enhanced gut health status of broilers under necrotic enteritis condition

Keerqin, Chake; Rhayat, Lamya; Zhang, Z.-H.; Gharib-Naseri, Kosar; Kheravii, Sarbast K.; Devillard, Estelle; Crowley, Tamsyn M.; Wu, Shu‐Biao

doi:10.1016/j.psj.2021.01.004

Cited by 27 publications

(17 citation statements)

References 70 publications

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“…Furthermore, birds fed PE and AM had downregulated CLDN5 gene compared to the CC group in male birds but the expression of CLDN5 gene was not different between birds fed PE and AM. These results were consistent with previous findings in broilers where birds fed antimicrobials had downregulated CLDN5 gene compared to the birds fed without additives under sub-clinical NE [ 11 ] and clinical NE challenged conditions [ 43 ]. These results indicate the similar effects of PE supplementation to AM implying its positive role in protecting the intestinal barrier of birds from pathogenic bacterial infection.…”

Section: Discussionsupporting

confidence: 93%

“…In this study, the caecal microbiota structure and composition were analysed via 16S rRNA sequencing to further investigate the mode of action underlying PE in alleviating NE-induced infection in the intestine. The results observed in the present study showed that NE challenge significantly decreased alpha diversity indicated by richness and evenness as also shown before Keerqin et al [ 11 ]. In addition, PCoA and RDA analyses showed that NE challenge altered beta diversity where the CC and UC were distinctly grouped.…”

Section: Discussionsupporting

confidence: 91%

“…The microbiota profoundly influenced the physiological, nutritional, metabolic and immunological status of the host. It is widely accepted that enteric diseases such as NE alters the microbiota composition of birds in the small intestine [ 4 , 11 , 68 , 69 ]. Such microbiota changes during disease outbreaks may indicate the immune-modulatory and inflammatory responses of the intestine.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, NE damages intestinal mucosa and barrier functions by altering the expression of genes coding tight junction proteins. It also changes the microbial community balance and reduces diversity which leads to a disruption of balanced microbial inhabitants [ 9 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

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A Microencapsulated Mixture of Eugenol and Garlic Tincture Supplementation Mitigates the Effect of Necrotic Enteritis on Intestinal Integrity and Increases Goblet Cells in Broilers

Kumar

Kheravii

Ionescu³

et al. 2021

Microorganisms

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This study was conducted to examine the effects of a plant extract mixture, a microencapsulated product composed of eugenol and garlic tincture (PE), on intestinal health in broilers under necrotic enteritis (NE) challenge. A total of 960 d-old mixed-sex Cobb 500 chicks were randomly distributed to 48-floor pens housing 20 birds per pen. Six treatments were applied: UC, unchallenged control; CC, challenged control; PE, challenged group plus PE; AM, challenged group plus antimicrobial (AM); FAP, challenged group plus a full dose of AM with PE; HAP, challenged group plus a half dose of AM with PE in starter, grower and finisher phases. Birds in the challenged groups were inoculated with Eimeria spp. on d 9 and Clostridium perfringens on d14. On d 16, the CC group had increased serum fluorescein isothiocyanate dextran (FITC-d), reduced villus surface area, goblet cell number, upregulated CLDN1, JAM2 genes and reduced microbial diversity compared to the UC group (p < 0.05). Birds fed PE had reduced FITC-d, increased goblet cell number and Bifidobacterium compared to the CC group (p < 0.05). Birds fed PE had reduced CLDN5 expression in male birds, and Bacteroides spp. in female birds than CC group (p < 0.05). These findings suggest that PE supplementation mitigates the effect of NE by improving the intestinal health of birds.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Microencapsulated Mixture of Eugenol and Garlic Tincture Supplementation Mitigates the Effect of Necrotic Enteritis on Intestinal Integrity and Increases Goblet Cells in Broilers

Kumar

Kheravii

Ionescu³

et al. 2021

Microorganisms

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“…Keerqin et al found that Bacillus subtilis 29,784 significantly improved broilers weight gain and enhanced their gut health status, as well as increased the abundance of Oscillospira in the intestinal tract. 16 Liu et al added Bacillus subtilis to the diets of pullets, which was able to improve the growth performance and intestinal function and induce Oscillospira to gradually become the dominant species in the gut. 76 Similarly, during neonatal broiler chicken rearing, the addition of probiotic bacillus preparations ( Bacillus amyloliquefaciens plus Bacillus subtilis ) increased the Oscillospira abundance and significantly reduced the horizontal transmission of pathogenic E. coli , and alleviated the severity of the infection.…”

Section: Factors Affecting the Abundance Of Oscillospiramentioning

confidence: 99%

Oscillospira - a candidate for the next-generation probiotics

et al. 2021

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Oscillospira is a class of organism that often appears in high-throughput sequencing data but has not been purely cultured and is widely present in the animal and human intestines. There is a strong association between variation in Oscillospira abundance and obesity, leanness, and human health. In addition, a growing body of studies has shown that Oscillospira is also implicated in other diseases, such as gallstones and chronic constipation, and has shown some correlation with the positive or negative changes in its course. Sequencing data combined with metabolic profiling indicate that Oscillospira is likely to be a genus capable of producing short-chain fatty acids (SCFAs) such as butyrate, which is an important reference indicator for screening “next-generation probiotics “. Considering the positive effects of Oscillospira in some specific diseases, such as obesity-related metabolic diseases, it has already been characterized as one of the next-generation probiotic candidates and therefore has great potential for development and application in the future food, health care, and biopharmaceutical products.

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Selenium-enriched Bacillus subtilis Improves Growth Performance, Antioxidant Capacity, Immune Status, and Gut Health of Broiler Chickens

Qiu

Gao

Hou

et al. 2023

Biol Trace Elem Res

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This study aimed to investigate the effects of selenium (Se) -enriched Bacillus subtilis (Se-BS) on growth performance, antioxidant capacity, immune status and gut health in broilers. A total of 240 day-old Arbor Acres broilers were randomly allotted to four treatments of a basal diet supplemented with no Se, sodium selenite (SS), B. subtilis (BS), and Se-BS and raised for 42 days. The results showed that Se-BS supplementation signi cantly increased body weight (BW), average daily gain, the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and peroxidase (POD), total antioxidant capacity (T-AOC), interleukin (IL) -2, IL-4 and immunoglobulin (Ig) G contents in plasma, the index and wall thickness of duodenum, the villus height and crypt depth of jejunum, GPx-1 and thioredoxin reductase 1 (TR-1) mRNA levels in liver and intestine (P < 0.05), and decreased feed conversion ratio (FCR) and malondialdehyde (MDA) content in plasma (P < 0.05). Compared with the SS and BS groups, Se-BS supplementation signi cantly increased BW, the activities of GPx, CAT and POD, and the contents of IL-2, IL-4 and IgG in plasma, the index and wall thickness of duodenum, crypt depth and sIgA content of jejunum, and GPx-1 mRNA level in liver and intestine (P < 0.05), and decreased FCR and MDA content in plasma (P < 0.05). This study provides scientifc basis for further understanding Se-BS as a more effective measure to improve growth performance and healthy of broilers.

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Probiotic Bacillus subtilis 29,784 improved weight gain and enhanced gut health status of broilers under necrotic enteritis condition

Cited by 27 publications

References 70 publications

A Microencapsulated Mixture of Eugenol and Garlic Tincture Supplementation Mitigates the Effect of Necrotic Enteritis on Intestinal Integrity and Increases Goblet Cells in Broilers

A Microencapsulated Mixture of Eugenol and Garlic Tincture Supplementation Mitigates the Effect of Necrotic Enteritis on Intestinal Integrity and Increases Goblet Cells in Broilers

Oscillospira - a candidate for the next-generation probiotics

Selenium-enriched Bacillus subtilis Improves Growth Performance, Antioxidant Capacity, Immune Status, and Gut Health of Broiler Chickens

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