Probiotic as an adjuvant therapy in chronic urticaria: a blinded randomized controlled clinical trial

Atefi, Najmessadat; Fallahpour, Morteza; Sharifi, S; Ghassemi, Mohammadreza; Roohaninasab, Masoumeh; Goodarzi, A.

doi:10.23822/eurannaci.1764-1489.200

Cited by 16 publications

(22 citation statements)

References 28 publications

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“…On the other hand, symbiotic bacteria establish a mutually beneficial relationship with the host. Some probiotics may become adjuvant therapies in the treatment of certain diseases ( Zhu and Liu, 2017 ; Atefi et al, 2021 ; Xu et al, 2021 ). Therefore, typing pathogenic and probiotic bacteria at the strain level is a tremendous challenge relevant to human health.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of IR Biotyper for Lactiplantibacillus plantarum Typing and Its Application Potential in Probiotic Preliminary Screening

Zhu

Wang

et al. 2022

Front. Microbiol.

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IR Biotyper (IRBT), which is a spectroscopic system for microorganism typing based on Fourier transform infrared (FTIR) technology, has been used to detect the spread of clones in clinical microbiology laboratories. However, the use of IRBT to detect probiotics has rarely been reported. Herein, we evaluated the discriminatory power of IRBT to type Lactiplantibacillus plantarum isolates at the strain level and explored its application potential in probiotic preliminary selection. Twenty Lactiplantibacillus isolates collected from pickled radishes during successive fermentation were used to test the robustness of IRBT at the strain level. IRBT was then compared with genotyping methods such as whole-genome sequencing (WGS), pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST) to evaluate its discrimination power. IRBT distributed the 20 isolates into five clusters, with L. argentoratensis isolate C7-83 being the most distant from the other isolates, which belonged to L. plantarum. IRBT showed good reproducibility, although deviation in the discriminative power of IRBT was found at the strain level across laboratories, probably due to technical variance. All examined methods allowed bacterial identification at the strain level, but IRBT had higher discriminatory power than MLST and was comparable to the WGS and PFGE. In the phenotypic comparison study, we observed that the clustering results of probiotic physiological attributes (e.g., sensitivity to acid and bile salts, hydrophobicity of the cell surface, and resistance to antibiotics) were consistent with the typing results of IRBT. Our results indicated that IRBT is a robust tool for L. plantarum strain typing that could improve the efficiency of probiotic identification and preliminary screening, and can potentially be applied in probiotic traceability and quality control.

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Section: Discussionmentioning

confidence: 99%

Evaluation of IR Biotyper for Lactiplantibacillus plantarum Typing and Its Application Potential in Probiotic Preliminary Screening

Zhu

Wang

et al. 2022

Front. Microbiol.

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“…These results suggest that uncovering the variability of gut flora by sub-classifying the patients according to certain clinical characteristics is warranted. In addition, the evidence that correction of gut dysbiosis in adult RCSU or CSU patients using adjunct therapy with probiotics only achieved limited efficacy based on the UAS7 score implies probiotics supplementation may not always work ( Nettis et al., 2016 ; Atefi et al., 2021 ). It may be reasonable as gut Bifidobacterium and Lactobacillus were not reduced in all patients, as was the case in the current study.…”

Section: Discussionmentioning

confidence: 99%

Altered Gut Microbiota in H1-Antihistamine-Resistant Chronic Spontaneous Urticaria Associates With Systemic Inflammation

Song

Dan

Yao

et al. 2022

Front. Cell. Infect. Microbiol.

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Background and ObjectiveChronic spontaneous urticaria (CSU) is a histamine-mediated inflammatory skin disease, and second-generation non-sedating H1-antihistamines (nsAH) at licensed doses have long been the first-line therapy in CSU. However, about 50% of patients are resistant to nsAH, and the precise pathogenesis remains largely unknown but seems to be associated with low-level systemic or intestinal inflammation. We aim to determine the fecal microbial composition and clarify its correlation with the clinical profiles og CSU with nsAH resistance.MethodsA total of 25 CSU patients with or 19 CSU patients without nsAH resistance and 19 healthy controls (HC) were enrolled in this study. The intestinal microbiome was detected by 16S rRNA sequencing. The data were analyzed using R language software.ResultsSignificantly higher urticarial activity score for 7 days, stool calprotectin, erythrocyte sedimentation rate, serum C-reactive protein, and interleukin-6, but much lower alpha-diversity and evenness of fecal bacterial community were observed in CSU patients with nsAH resistance than in those without (P <0.05 for all variables). Compared to patients with nsAH-responsiveness, the abundance of fecal genera Prevotella, Megamonas, and Escherichia were significantly increased, while that of Blautia, Alistipes, Anaerostipes, and Lachnospira were remarkably reduced in nsAH-resistant patients (uncorrected P <0.05 for all variables). Finally, systemic not intestinal inflammation degree was positively correlated with genera Escherichia, while negatively with genera Blautia, Dorea, Lactobacillus, Eubacterium_hallii_group, and Roseburia. CSU without nsAH resistance and HC individuals showed almost unchanged genera bacterium.ConclusionsAmong CSU patients, pro-inflammation phenotype relating to enteric dysbacteriosis features nsAH resistance in CSU patients. The results provide clues for future microbial-based or anti-inflammatory therapies on nsAH resistant CSU.

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“…In our research, probiotics were administered three hours after IV administration of antibiotics to evade the effect of antibiotics on probiotics. [13][14] . They improved in 36.6% of subjects compare with only 5.8% in the group of placebo.…”

Section: Discussionmentioning

confidence: 99%

Role of Multistrain Probiotic as Supportive Therapy in Reducing the Frequency and Severity of Respiratory Infections in Children

Farrukh¹,

Masood²,

Naseer³

et al. 2021

PJMHS

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Background: Respiratory tract infection is a disease that can result in high mortality and morbidity. Factors related with severe respiratory infections in children comprise the age of young children, male sex, malnutrition, incomplete vaccination status and breastfeeding only children. This analysis targets the Role of multi-strain probiotics as supportive therapy in reducing the frequency and severity of respiratory infections among children. Place and Duration: In the Pediatric Medicine department of Abbasi Shaheed Hospital, Karachi for six months duration from January 2021 to June 2021. Methods: This was a randomized controlled study in 74 children aged 2 months to 5 years with respiratory infections receiving standard therapy and multi strains probiotics versus standard therapy and placebo. The assessment regarding treatment was done in both groups. Results following 7 days of adjuvant treatment alienated into group I (n = 37) (standard therapy and multi strains probiotics) and II Group (n = 37) (placebo and standard therapy). The factors evaluated in this analysis included subject characteristics, duration of stay, duration of fever, dyspnea, withdrawal, C-reactive protein, rales, breast history and probiotics use. The analysis of data was accomplished using SPSS version 20.0. Results: The males were 51.4% and females were 49.6%), breastfeeding (83.8% in Group-I vs 67.6% in Group-II), children who were not malnourished (81.1% in Group-I vs 86.5% in Group-II), and children who completed vaccination history (91.9% in Group-I versus 78.4% in Group-II). As shown in Table 1 in groups I and II, correspondingly, the mean concentration of CRP in subjects diagnosed with respiratory infections was not statistically significant in 1st group (30.10 (20.01-44.60 mg / l), relative to Group II (28.23 (19.20-46.12 mg / l), respectively (P = 0.39).Multivariate analysis with Ancova showed that probiotics can significantly decrease the time of Rale by 5.84 hours (p = 0.021, CI 95% -10.90 - (- 0.87). Conclusions: In this study, it was found that multi strains probiotic therapy significantly reduced the duration of Rale among children with infections of the upper respiratory tract. Keywords: Respiratory tract infections, children, multi strain probiotics, efficiency.

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Probiotic as an adjuvant therapy in chronic urticaria: a blinded randomized controlled clinical trial

Cited by 16 publications

References 28 publications

Evaluation of IR Biotyper for Lactiplantibacillus plantarum Typing and Its Application Potential in Probiotic Preliminary Screening

Evaluation of IR Biotyper for Lactiplantibacillus plantarum Typing and Its Application Potential in Probiotic Preliminary Screening

Altered Gut Microbiota in H1-Antihistamine-Resistant Chronic Spontaneous Urticaria Associates With Systemic Inflammation

Role of Multistrain Probiotic as Supportive Therapy in Reducing the Frequency and Severity of Respiratory Infections in Children

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