Probiotic alleviate fluoride-induced memory impairment by reconstructing gut microbiota in mice

Xin, Jinge; Wang, Hesong; Sun, Ning; Bughio, Shamsuddin; Zeng, Dong; Li, Lianxin; Wang, Yanyan; Khalique, Abdul; Zeng, Yan; Pan, Kangcheng; Jing, Bo; Ma, Hua; Bai, Yang

doi:10.1016/j.ecoenv.2021.112108

Cited by 26 publications

(18 citation statements)

References 53 publications

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“…Used qPCR to determine the relative expression abundance between IL-1β, IL-6, IL-10, IFN-γ, TNF-α and the internal reference gene β-actin in small intestine ( 34 ). The primer sequences used for qPCR are shown in Supplementary Table S3 .…”

Section: Methodsmentioning

confidence: 99%

Surface Display of porcine circovirus type 2 antigen protein cap on the spores of bacillus subtilis 168: An effective mucosal vaccine candidate

Li²,

Dai³

et al. 2022

Front. Immunol.

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The oral mucosal vaccine has great potential in preventing a series of diseases caused by porcine circovirus type 2 (PCV2) infection. This study constructed a recombinant Bacillus subtilis RB with PCV2 Capsid protein (Cap) on its spore surface and cotB as a fusion partner. The immune properties of the recombinant strain were evaluated in a mouse model. IgA in intestinal contents and IgG in serum were detected by enzyme-linked immunosorbent assay (ELISA). The results demonstrated that recombinant spores could activate strong specific mucosal and humoral immune responses. In addition, spores showed good mucosal immune adjuvant function, promoting the proliferation of CD3+, CD4+ and CD8+ T cells and other immune cells. We also found that the relative expression of inflammatory cytokines such as IL-1b, IL-6, IL-10, TNF-a and IFN in the small intestinal mucosa was significantly up-regulated under the stimulation of recombinant bacteriophage. These effects are important for the balance of Th1/Th2-like responses. In summary, our results suggest that recombinant B. subtilis RB as a feed additive provides a new strategy for the development of novel and safe PCV2 mucosal subunit vaccines.

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Section: Methodsmentioning

confidence: 99%

Surface Display of porcine circovirus type 2 antigen protein cap on the spores of bacillus subtilis 168: An effective mucosal vaccine candidate

Li²,

Dai³

et al. 2022

Front. Immunol.

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“…34 Furthermore, the probiotic Lactobacillus johnsonii BS15 was shown to mitigate fluoride-induced recognition memory deficits by restoring gut microbiota and reducing intestinal permeability and inflammatory cytokines TNF-α and IL-1β in mice. 44 In addition, Mohammadi et al ( 2019) have revealed that L. helveticus and B. longum can attenuate memory deficits, as well as serum and hippocampal levels of TNF-α and IL-1β in LPS-treated rats. 28 A human study has reported the safety and efficacy of the probiotic Bifidobacterium breve in ameliorating memory functions in elderly individuals with suspected mild cognitive impairment.…”

Section: ■ Discussionmentioning

confidence: 99%

“…thermophilus were found to improve learning and memory dysfunction in d -galactose-treated mice by inhibiting neuronal apoptosis and brain injury . Furthermore, the probiotic Lactobacillus johnsonii BS15 was shown to mitigate fluoride-induced recognition memory deficits by restoring gut microbiota and reducing intestinal permeability and inflammatory cytokines TNF-α and IL-1β in mice . In addition, Mohammadi et al (2019) have revealed that L.…”

Section: Discussionmentioning

confidence: 99%

Preventive Effect of Saccharomyces boulardii on Memory Impairment Induced by Lipopolysaccharide in Rats

Babaei

Mirzababaei

Dargahi

et al. 2022

ACS Chem. Neurosci.

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Recent studies have indicated that dysfunction of gut microbiota, living microorganisms of the digestive tract, plays a role in the pathogenesis of neurodegenerative disorders, indicating the valuable impact of probiotics as a potential preventive or therapeutic strategy. Saccharomyces boulardii is a yeast probiotic with beneficial effects on various disorders, ranging from inflammatory gastrointestinal diseases to brain and behavioral disorders. Herein, we examined the effect of S. boulardii on memory impairment induced by lipopolysaccharide (LPS) in Wistar rats. Four groups of rats were used in this study (N = 10): (1) control [Cnt], (2) LPS, (3) LPS + S. boulardii [LPS + S], and (4) S. boulardii [S]. Animals were orally administered S. boulardii (250 mg/ rat) or saline by gavage for 4 weeks. From the 14th day of the study, animals were administered intraperitoneal LPS (0.25 mg/kg/ day) or saline for 9 days. We assessed memory impairment, neuroinflammation, and amyloid-β deposition. S. boulardii ameliorated LPS-induced memory dysfunction. We observed that S. boulardii significantly reduced the elevated levels of serum interleukin (IL)-1β, IL-6, and tumor necrosis factor-α, as well as hippocampal levels of NLRP3 and caspase-1 in the LPS model. Moreover, S. boulardii alleviated amyloid-β deposition in the rat hippocampus. Collectively, our findings indicated that S. boulardii could inhibit memory impairment, neuroinflammation, and amyloid-β accumulation induced by LPS, possibly by modifying the gut microbiota.

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“…Our study also verified the preventative influence of L. johnsonii BS15 on drunkenness. The normal animal memory level relies upon a delicate change in the intestinal flora, organizational barrier, and circulation system (Sun et al, 2020;Xin et al, 2020Xin et al, , 2021aWang et al, 2021). Although various factors, such as neuroinflammation, lipid peroxidation, autophagy, and apoptosis, can affect brain health, the gut microbiome plays a significant role as one of the main drivers.…”

Section: Discussionmentioning

confidence: 99%

Psychoactive Effects of Lactobacillus johnsonii BS15 on Preventing Memory Dysfunction Induced by Acute Ethanol Exposure Through Modulating Intestinal Microenvironment and Improving Alcohol Metabolic Level

et al. 2022

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The negative effects of ethanol (EtOH) abuse on the body have been widely reported in recent years. Building on the microbiota-gut-brain axis hypothesis, our study aimed to demonstrate the potential psychobiotic role of Lactobacillus johnsonii BS15 in the preventive effects of acute EtOH intake on memory impairment. We also determined whether L. johnsonii BS15 intake could effectively improve resistance to acute drinking and alleviate the adverse effects of EtOH. Male mice were fed L. johnsonii BS15 orally with (Probiotic group) or without (Control and Alcohol groups) daily dose of 0.2 × 109 CFU/ml per mouse for 28 days. Gavage with L. johnsonii BS15 significantly modified the ileal microbial ecosystem (assessed by 16S rRNA gene sequencing) in favor of Firmicutes and Lactobacillus, indicating the ability of BS15 to restore the gut microbiota. The acute EtOH exposure model (7 g/kg EtOH per mice) was established by gavage, which was administered to the alcohol and probiotic groups on day 28 of the experiment. The L. johnsonii BS15 intake effectively reduced alcohol unconsciousness time, blood alcohol concentration, and serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels. Meanwhile, the improvement of ethanol resistance time and the activities of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) in the liver were shown by BS15 in acute alcohol-induced mice. We found that acute EtOH exposure reduced the exploration ratio (assessed by the novel object recognition test), escape latency, number of errors (assessed by passive avoidance test), and spontaneous exploration (assessed by T-maze test) in mice, which were obviously improved by L. johnsonii BS15. In the hippocampus, L. johnsonii BS15 significantly reversed the decrease in antioxidant capacity of superoxide dismutase (SOD), malondialdehyde (MDA), and glutathione (GSH) and mRNA expression of memory-related functional proteins of brain-derived neurotrophic factor (BDNF) and cyclic ampresponse element binding protein (CREB) in the hippocampal tissue after acute EtOH exposure. In conclusion, L. johnsonii BS15 intake appears as a promising psychoactive therapy to ameliorate alcohol-mediated memory impairment by increasing EtOH metabolic levels.

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Probiotic alleviate fluoride-induced memory impairment by reconstructing gut microbiota in mice

Cited by 26 publications

References 53 publications

Surface Display of porcine circovirus type 2 antigen protein cap on the spores of bacillus subtilis 168: An effective mucosal vaccine candidate

Surface Display of porcine circovirus type 2 antigen protein cap on the spores of bacillus subtilis 168: An effective mucosal vaccine candidate

Preventive Effect of Saccharomyces boulardii on Memory Impairment Induced by Lipopolysaccharide in Rats

Psychoactive Effects of Lactobacillus johnsonii BS15 on Preventing Memory Dysfunction Induced by Acute Ethanol Exposure Through Modulating Intestinal Microenvironment and Improving Alcohol Metabolic Level

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