Probing the weak interaction of proteins with neutral and zwitterionic antifouling polymers

Wu, Jiang; Zhao, Chao; Hu, Rundong; Lin, Weifeng; Wang, Qiuming; Zhao, Jun; Bilinovich, Stephanie M.; Leeper, Thomas C.; Li, Lingyan; Cheung, H. Michael; Chen, Shengfu; Zheng, Jie

doi:10.1016/j.actbio.2013.09.038

Cited by 70 publications

(47 citation statements)

References 53 publications

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“…These fluorophores can be excited at the wavelength (λ exc ) of ~285 nm; however most of the fluorescence attributed to Trp because of the efficient resonance energy transfer (RET) from tyrosine to tryptophan [46]. Since the Trp fluorophore can be excited at the wavelength (λ exc ) of ~285 nm, the excitation of all other amino acids in the protein can be avoided as these absorb at shorter wavelength [20]. The fluorescence of Trp in the protein is sensitive to environmental changes such as polarizability [47], and thus it can be used as an optical probe to analyze the effect of co-solute on the conformational changes of the protein [48].…”

Section: Resultsmentioning

confidence: 99%

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Exploring the Behavior of Bovine Serum Albumin in Response to Changes in the Chemical Composition of Responsive Polymers: Experimental and Simulation Studies

et al. 2016

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Knowledge of the interactions between polymer and protein is very important to fabricate the potential materials for many bio-related applications. In this regard, the present work investigated the effect of copolymers on the conformation and thermal stability of bovine serum albumin (BSA) with the aid of biophysical techniques such as fluorescence spectroscopy, circular dichroism (CD) spectroscopy and differential scanning calorimetry (DSC). In comparison with that of copolymer PGA-1.5, our fluorescence spectroscopy results reveal that the copolymer PGA-1, which has a lower PEGMA/AA ratio, shows greater influence on the conformation of BSA. Copolymers induced unfolding of the polypeptide chain of BSA, which was confirmed from the loss in the negative ellipticity of CD spectra. DSC results showed that the addition of PGA-1 and PGA-1.5 (0.05% (w/v) decreased the transition temperature by 14.8 and 11.5˝C, respectively). The results from the present study on the behavior of protein in response to changes in the chemical composition of synthetic polymers are significant for various biological applications such as enzyme immobilization, protein separations, sensor development and stimuli-responsive systems.

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Section: Resultsmentioning

confidence: 99%

“…The stability of horseradish peroxidase (HRP) was improved upon incorporation in PNIPAM-based hydrogels [17]. Generally speaking, these proteins interacted with the polymeric materials through hydrophobic interactions, electrostatic interactions and hydrogen bonds [20].…”

Section: Introductionmentioning

confidence: 99%

Exploring the Behavior of Bovine Serum Albumin in Response to Changes in the Chemical Composition of Responsive Polymers: Experimental and Simulation Studies

et al. 2016

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“…The reduction of platelet deposition associated with SB block copolymer conjugation are likely related to changes of the surface properties and the reduced protein (e.g., fibrinogen) adsorption, as confirmed in our previous study. 52 The antifouling mechanisms associated with zwitterion-modified surfaces have also been extensively considered in terms of surface property changes (interactions with water and salts) in previous publications 53,54 while the antifouling effectiveness on the SB modified surface could be variable based upon salt concentration, buffer composition, solution pH level and temperature. 55,56 The majority of studies developing thromboresistant coatings have focused on a single material and application.…”

Section: Figurementioning

confidence: 99%

Development of zwitterionic sulfobetaine block copolymer conjugation strategies for reduced platelet deposition in respiratory assist devices

Malkin

Lee

et al. 2018

J Biomed Mater Res

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Respiratory assist devices, that utilize ∼2 m of hollow fiber membranes (HFMs) to achieve desired gas transfer rates, have been limited in their adoption due to such blood biocompatibility limitations. This study reports two techniques for the functionalization and subsequent conjugation of zwitterionic sulfobetaine (SB) block copolymers to polymethylpentene (PMP) HFM surfaces with the intention of reducing thrombus formation in respiratory assist devices. Amine or hydroxyl functionalization of PMP HFMs (PMP-A or PMP-H) was accomplished using plasma-enhanced chemical vapor deposition. The generated functional groups were conjugated to low molecular weight SB block copolymers with N-hydroxysuccinimide ester or siloxane groups (SBNHS or SBNHSi) that were synthesized using reversible addition fragmentation chain transfer polymerization. The modified HFMs (PMP-A-SBNHS or PMP-H-SBNHSi) showed 80-95% reduction in platelet deposition from whole ovine blood, stability under the fluid shear of anticipated operating conditions, and uninhibited gas exchange performance relative to non-modified HFMs (PMP-C). Additionally, the functionalization and SBNHSi conjugation technique was shown to reduce platelet deposition on polycarbonate and poly(vinyl chloride), two other materials commonly found in extracorporeal circuits. The observed thromboresistance and stability of the SB modified surfaces, without degradation of HFM gas transfer performance, indicate that this approach is promising for longer term pre-clinical testing in respiratory assist devices and may ultimately allow for the reduction of anticoagulation levels in patients being supported for extended periods. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2681-2692, 2018.

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“…Besides, inspired by the self‐cleaning effect of fish skin (such as skin of sharks), various fouling‐resistant hydrophilic surfaces have been conceived as more promising candidates for preventing the attachment of biofoulants (not only in marine application but even more commonly in the biomedical and biotechnology fields) because of its low interfacial energy with water. This makes the interaction between microorganism and substrate is thermodynamically unfavorable since the highly hydrated surface increases the energetic barrier for replacing the combined water molecules with microorganisms . At present, the dependence of antibiofouling behavior on film physicochemical properties has been investigated mainly in protein adsorption, bacteria settlement, and cell proliferation behavior .…”

Section: Introductionmentioning

confidence: 99%

Enhancement of graft density and chain length of hydrophilic polymer brush for effective marine antifouling

Yang

Zhang

Yang

et al. 2018

J of Applied Polymer Sci

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2-Hydroxyethyl methacrylate polymer brushes with various grafting densities and chain lengths were prepared through surface-initiated atom transfer radical polymerization. X-ray photoelectron spectra, ellipsometry measurement, contact angle measurement, and atom force microscope were used to characterize the prepared polymer brush. The biofouling assays of polymer brush were investigated by adhesion of Dunaliella tertiolecta, Navcular sp., and Bovine Serum Albumin protein and by static marine immersion field test. Besides, hydroxyl and sulfonate-terminated self-assembled monolayers, anionic charged 3-sulfopropyl methacrylate potassium salt polymer brush were prepared for comparison. Results suggest that the settlement of microorganisms can be largely reduced by polymer with enough polymer chain length and grafting density. More importantly, static immersion field tests indicate that hydrophilic polymer film with enough hydration layer thickness is necessary for long-term marine antifouling application. This comprehensive investigation is of great importance to understanding their influence on the adhesion of marine microorganism.

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Probing the weak interaction of proteins with neutral and zwitterionic antifouling polymers

Cited by 70 publications

References 53 publications

Exploring the Behavior of Bovine Serum Albumin in Response to Changes in the Chemical Composition of Responsive Polymers: Experimental and Simulation Studies

Exploring the Behavior of Bovine Serum Albumin in Response to Changes in the Chemical Composition of Responsive Polymers: Experimental and Simulation Studies

Development of zwitterionic sulfobetaine block copolymer conjugation strategies for reduced platelet deposition in respiratory assist devices

Enhancement of graft density and chain length of hydrophilic polymer brush for effective marine antifouling

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