Probing the single and combined toxicity of PFOS and Cr(VI) to soil bacteria and the interaction mechanisms

Li, Jie; Zheng, Tongtong; Yuan, Dong; Gao, Canzhu; Liu, Chunguang

doi:10.1016/j.chemosphere.2020.126039

Cited by 33 publications

(6 citation statements)

References 43 publications

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“…Scanning electron microscopy was used to observe the morphology of the bacterium: the LBA119 bacteria were fixed in 1% glutaraldehyde solution for 20 min and then centrifuged, rinsed and centrifuged several times using phosphate buffer, then dehydrated (3–4 times) with increasing concentrations of ethanol (30%, 50%, 70%, 80%, 90%, 100%) step by step, and finally the ethanol solution containing bacteria was dropped on clean polished aluminum sheets, dried, sprayed with gold, and microscopic images of bacterial cells were observed with scanning electron microscopy. The size of individual organisms was approximately 0.8 × 1.3 μm [ 18 ] under electron microscopy ( Figure 1 c).…”

Section: Resultsmentioning

confidence: 99%

Isolation and Identification of Mercury-Tolerant Bacteria LBA119 from Molybdenum-Lead Mining Soils and Their Removal of Hg2+

Yao

Wang

et al. 2023

Toxics

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Aims: To screen heavy metal-tolerant strains from heavy metal-contaminated soil in mining areas and determine the tolerance of the strains to different heavy metals and their removal rates through experiments. Methods: Mercury-resistant strain LBA119 was isolated from mercury-contaminated soil samples in Luanchuan County, Henan Province, China. The strain was identified by Gram staining, physiological and biochemical tests, and 16S rDNA sequences. The LBA119 strain showed good resistance and removal rates to heavy metals such as Pb2+, Hg2+, Mn2+, Zn2+, and Cd2+ using tolerance tests under optimal growth conditions. The mercury-resistant strain LBA119 was applied to mercury-contaminated soil to determine the ability of the strain to remove mercury from the soil compared to mercury-contaminated soil without bacterial biomass. Results: Mercury-resistant strain LBA119 is a Gram-positive bacterium that appears as a short rod under scanning electron microscopy, with a single bacterium measuring approximately 0.8 × 1.3 μm. The strain was identified as a Bacillus by Gram staining, physiological and biochemical tests, and 16S rDNA sequence analysis. The strain was highly resistant to mercury, with a minimum inhibitory concentration (MIC) of 32 mg/L for mercury. Under a 10 mg/L mercury environment, the optimal inoculation amount, pH, temperature, and salt concentration of the LBA119 strain were 2%, 7, 30 °C, and 20 g/L, respectively. In the 10 mg/L Hg2+ LB medium, the total removal rate, volatilization rate, and adsorption rate at 36 h were 97.32%, 89.08%, and 8.24%, respectively. According to tolerance tests, the strain showed good resistance to Pb2+, Mn2+, Zn2+, Cd2+, and other heavy metals. When the initial mercury concentration was 50 mg/L and 100 mg/L, compared with the mercury-contaminated soil that contained an LB medium without bacterial biomass, LBA119 inoculation increased 15.54–37.67% after 30 days of culture. Conclusion: This strain shows high bioremediation potential for mercury-contaminated soil.

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Section: Resultsmentioning

confidence: 99%

Isolation and Identification of Mercury-Tolerant Bacteria LBA119 from Molybdenum-Lead Mining Soils and Their Removal of Hg2+

Yao

Wang

et al. 2023

Toxics

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“…58 It should be reminded that PFOS molecules are negatively charged due to sulfonic acid functional groups (Figure S2). 59 Due to their higher electronegative charge of MXene-TFN compared to bare TFC at every pH, the functionalization of MXene nanosheets onto the PA selective layer would result in the enhancement of the electrostatic interactions of the TFC membrane. 41 The TFC-0.005 MXene membranes showed the most enhanced negative surface charge among the membranes with more than 3 times higher negative surface charge than bare TFC.…”

Section: Acs Applied Materials and Interfacesmentioning

confidence: 99%

Functionalized-MXene Thin-Film Nanocomposite Hollow Fiber Membranes for Enhanced PFAS Removal from Water

Jamshidi

Beidaghi

et al. 2022

ACS Appl. Mater. Interfaces

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Due to adverse health effects and the broad sources of per-and polyfluoroakyl substances (PFAS), PFAS removal is a critical research area in water purification. We demonstrate the functionalization of thin-film composite (TFC) hollow fiber nanofiltration (HFN) membranes by MXene nanosheets during the interfacial polymerization (IP) process for enhanced removal of perfluorooctane sulfonic acid (PFOS) from water. A MXenepolyamide (PA) selective layer was fabricated on top of a polysulfone (PSF) hollow fiber support via IP of trimesoyl chloride (TMC) and a mixture of piperazine (PIP) and MXene nanosheets to form MXene-PA thin-film nanocomposite (TFN) membranes. Incorporating MXene nanosheets during the IP process tuned the morphology and negative surface charge of the selective layer, resulting in enhanced PFOS rejection from 72% (bare TFC) to more than 96% (0.025 wt % MXene TFN), while the water permeability was also increased from 13.19 (bare TFC) to 29.26 LMH/bar (0.025 wt % MXene TFN). Our results demonstrate that both electrostatic interaction and size exclusion are the main factors governing the PFOS rejection, and both are determined by PA selective layer structural and chemical properties. The lamella structure and interlayer of MXene nanosheets inside the PA layer provided different transport mechanisms for water, ions, and PFAS molecules, resulting in enhanced water permeability and PFAS rejection due to traveling through the membrane by both diffusions through the PA layer and the MXene intralayer channels. MXene nanosheets showed very promising capability as a 2D additive for tuning the structural and chemical properties of the PA layer at the permeability-rejection tradeoff.

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“…155765). In brief, control bacterial cultures and culture with Cr(VI) 100 mg/L at 35°C for 48 h were harvested at 10,000 × g for 10 min followed by resuspended with PBS (pH 7.4) to minimize the reign of pH (Li et al 2020).…”

Section: Antioxidant Assaymentioning

confidence: 99%

Mechanism of Cr(VI) reduction by an indigenous Rhizobium pusense CR02 isolated from chromite mining quarry water (CMQW) at Sukinda Valley, India

Sahoo

Kisku

Varadwaj

et al. 2022

Environ Sci Pollut Res

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Toxicological assessment of CMQW generated due to chromite mining activities at Sukinda, valley has revealed high chromium contamination along with Zn and Fe. The present study focused on mechanism of chromate reduction by an indigenous multi-metal tolerant bacterium Rhizobium pusense CR02 isolated from CMQW. Isolated strain has shown resistance upto (520 mg/L) of Cr(VI) with IC50 value of 385.4 mg/L. The highest reduction rate 8.6×10 -2 /h was recorded with 20 mg/L of initial concentration of Cr(VI). Extracellular (3.06±0.012 U/mL), intracellular (3.60±0.13 U/mL) and membrane (1.89±0.01 U/mL) associated chromate reductases were found to be involved for reduction. The extracellular polymeric substances (EPS) produced by the isolate also enhanced reduction activity of 46.32±1.69 mg/L after 72 h with initial concentration of 50 mg/L. FTIR analysis revealed the involvement of functional groups -OH, -CO and -NH for Cr(VI) biosorption whereas P=O, -CO-NH-and -COOH interacted with Cr(III). Zeta potential with less negative surface charge favored reduction of Cr(VI). Treatment of CMQW by bacterial isolate detoxi ed Cr(VI) minimizing chromosomal aberrations in root cells of Allium cepa L., suggesting the role of Rhizobium pusense CR02 as a promising bio-agent for Cr(VI) detoxi cation. DeclarationsCredit author statement Hrudananda Sahoo: Conceptualization, methodology, execution of all experiments, data analysis, validation, manuscript writing, review and editing. Kanika Kisku, Kumar S. K. Varadwaj, and Prasannajit Acharya: Data analysis, review, and editing. Umesh Chandra Naik: Conceptualization, validation of data, review, and editing.Acknowledgments would like to express our sincere thanks to SERB, Govt. of India (YSS/2015/001089) for the nancial support. We would also acknowledge University Grants Commission (UGC), Govt. of India for providing Fellowship. We would also like to acknowledge CIF, OUAT, Bhubaneswar for ICP-OES and Zeta potential analysis; CSIR-NIIST, Thiruvananthapuram, Kerala for XPS analysis, and ILS, Bhubaneswar for DNA sequencing facilities. We are also highly thankful to the research

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Probing the single and combined toxicity of PFOS and Cr(VI) to soil bacteria and the interaction mechanisms

Cited by 33 publications

References 43 publications

Isolation and Identification of Mercury-Tolerant Bacteria LBA119 from Molybdenum-Lead Mining Soils and Their Removal of Hg2+

Isolation and Identification of Mercury-Tolerant Bacteria LBA119 from Molybdenum-Lead Mining Soils and Their Removal of Hg2+

Functionalized-MXene Thin-Film Nanocomposite Hollow Fiber Membranes for Enhanced PFAS Removal from Water

Mechanism of Cr(VI) reduction by an indigenous Rhizobium pusense CR02 isolated from chromite mining quarry water (CMQW) at Sukinda Valley, India

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