Abstract:ADP‐glucose pyrophosphorylase (ADPG PPase) catalyzes the rate limiting step in glucan synthesis pathways of bacteria and plants and is an attractive target for protein engineering to increase the production of renewable carbon. Agrobacterium tumefaciens (Ag.t.) ADPG PPase is activated by F6P and pyruvate and inhibited by Pi; these effectors are believed to bind at allosteric site(s) located between the catalytic N‐terminal and C‐terminal domains. We probed the role of highly conserved P288 in the loop region b… Show more
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