Probing the Mode of Antibacterial Action of Silver Nanoparticles Synthesized by Laser Ablation in Water: What Fluorescence and AFM Data Tell Us

Krce, Lucija; Šprung, Matilda; Rončević, Tomislav; Maravić, Ana; Čulić, Vedrana Čikeš; Blažeka, Damjan; Krstulović, Nikša; Aviani, Ivica

doi:10.3390/nano10061040

Cited by 14 publications

(8 citation statements)

References 67 publications

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“…Mechanistically, the prepared nanoparticles may cause bacterial membrane lysis either directly binding to the negatively charged portion of the lipid molecules or through the release of reactive oxygen species [ROS] which may further trigger intracellular damage through secondary messengers leading to a powerful bactericidal action. Similar mechanism has been anticipated for positively charged nanoparticles against various pathologic bacterial strains [31,32]. However, exact molecular nature of antibacterial action of AgNPs and BAC-AgNPs remains to unclear.…”

Section: Antibacterial Assayssupporting

confidence: 53%

Clinical Application of Silver Nanoparticles Coated by Benzalkonium Chloride

Ansari¹,

Alshanberi

2021

Coatings

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The present study investigates the surface modification of AgNPs (synthesized by neem leaves) by benzalkonium chloride (BAC). It was observed that 22 × 109 CFU were formed at 0.25 mM AgNPs concentration. However, it was reduced to 14 × 109 CFU for BAC-coated AgNPs at similar experimental conditions. The enzymatic activity of β-glucosidase was significantly enhanced from 0.0625 mM to 0.5 mM concentration of AgNPs, as well as BAC–AgNPs. However, there was no further change of activity beyond this concentration. ZOI of AgNPs and BAC–AgNPs was measured against E. coli, B. subtilis, P. aeruginosa, and S pneumoniae at 0.25 mM and 0.50 mM concentrations of these bioactive agents. ZOI was 3.45 cm and 3.56 cm for AgNPs and BAC–AgNPs at 0.25 mM of these bioactive agents, respectively, against E. coli. However, these values were 4.28 cm and 4.40 cm, respectively, against B. subtilis. ZOI was obtained at 3.36 cm and 3.47 cm, respectively, against P. aeruginosa under similar experimental concentrations. However, ZOI was achieved at 3.44 cm and 3.62 cm, respectively, against S. pneumonia, under similar experimental conditions. Hence, such research findings can be exploited for potential applications in numerous environmental and biomedical fields.

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Section: Antibacterial Assayssupporting

confidence: 53%

Clinical Application of Silver Nanoparticles Coated by Benzalkonium Chloride

Ansari¹,

Alshanberi

2021

Coatings

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“…Several researchers report that AgNPs promote the induction of ROS [50,57]. The relevant antimicrobial effect of AgNPs is attributed to its potential upregulation of ROS, leading to damage of the cell cytoskeleton, proteins, and nucleic acids that eventually results in the antibacterial effect [50,58] (Figure 1). CuNPs are also well known to be highly cytotoxic by the release of ions and the production of ROS [59].…”

Section: Metal-based Nanoparticles Target Ros Production In Bacteriamentioning

confidence: 99%

Current Knowledge on the Oxidative-Stress-Mediated Antimicrobial Properties of Metal-Based Nanoparticles

et al. 2022

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The emergence of multidrug-resistant (MDR) bacteria in recent years has been alarming and represents a major public health problem. The development of effective antimicrobial agents remains a key challenge. Nanotechnologies have provided opportunities for the use of nanomaterials as components in the development of antibacterial agents. Indeed, metal-based nanoparticles (NPs) show an effective role in targeting and killing bacteria via different mechanisms, such as attraction to the bacterial surface, destabilization of the bacterial cell wall and membrane, and the induction of a toxic mechanism mediated by a burst of oxidative stress (e.g., the production of reactive oxygen species (ROS)). Considering the lack of new antimicrobial drugs with novel mechanisms of action, the induction of oxidative stress represents a valuable and powerful antimicrobial strategy to fight MDR bacteria. Consequently, it is of particular interest to determine and precisely characterize whether NPs are able to induce oxidative stress in such bacteria. This highlights the particular interest that NPs represent for the development of future antibacterial drugs. Therefore, this review aims to provide an update on the latest advances in research focusing on the study and characterization of the induction of oxidative-stress-mediated antimicrobial mechanisms by metal-based NPs.

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“…The production of reactive oxygen species (ROS) is generally considered one of the main mechanisms for AgNPs’ antibacterial activity. However, this is controversial and Krce et al (2020) [ 81 ] have claimed that it is not the case, and rather that the effect on the membrane’s permeability is the main mechanism of antibacterial activity. However, the possibility still exists of ROS being one of the factors leading to the antibacterial activity of AgNPs.…”

Section: Resultsmentioning

confidence: 99%

Green Extracellular Synthesis of Silver Nanoparticles by Pseudomonas alloputida, Their Growth and Biofilm-Formation Inhibitory Activities and Synergic Behavior with Three Classical Antibiotics

et al. 2022

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Bacterial resistance to antibiotics is on the rise and hinders the fight against bacterial infections, which are expected to cause millions of deaths by 2050. New antibiotics are difficult to find, so alternatives are needed. One could be metal-based drugs, such as silver nanoparticles (AgNPs). In general, chemical methods for AgNPs’ production are potentially toxic, and the physical ones expensive, while green approaches are not. In this paper, we present the green synthesis of AgNPs using two Pseudomonas alloputida B003 UAM culture broths, sampled from their exponential and stationary growth phases. AgNPs were physicochemically characterized by transmission electron microscopy (TEM), total reflection X-ray fluorescence (TXRF), infrared spectroscopy (FTIR), dynamic light scattering (DLS), and X-ray diffraction (XRD), showing differential characteristics depending on the synthesis method used. Antibacterial activity was tested in three assays, and we compared the growth and biofilm-formation inhibition of six test bacteria: Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, and Staphylococcus epidermidis. We also monitored nanoparticles’ synergic behavior through the growth inhibition of E. coli and S. aureus by three classical antibiotics: ampicillin, nalidixic acid, and streptomycin. The results indicate that very good AgNP activity was obtained with particularly low MICs for the three tested strains of P. aeruginosa. A good synergistic effect on streptomycin activity was observed for all the nanoparticles. For ampicillin, a synergic effect was detected only against S. aureus. ROS production was found to be related to the AgNPs’ antibacterial activity.

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Probing the Mode of Antibacterial Action of Silver Nanoparticles Synthesized by Laser Ablation in Water: What Fluorescence and AFM Data Tell Us

Cited by 14 publications

References 67 publications

Clinical Application of Silver Nanoparticles Coated by Benzalkonium Chloride

Clinical Application of Silver Nanoparticles Coated by Benzalkonium Chloride

Current Knowledge on the Oxidative-Stress-Mediated Antimicrobial Properties of Metal-Based Nanoparticles

Green Extracellular Synthesis of Silver Nanoparticles by Pseudomonas alloputida, Their Growth and Biofilm-Formation Inhibitory Activities and Synergic Behavior with Three Classical Antibiotics

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