Probing the mechanical properties of TNF-&amp;alpha; stimulated endothelial cell with atomic force microscopy [Corrigendum]

Lee, Soo-Youn; Am, Zaske; Novellino, Tommaso

doi:10.2147/ijn.s65775

Cited by 3 publications

(3 citation statements)

References 8 publications

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“…The TNF‐α is a pro‐inflammatory cytokine that regulates the permeability of blood and lymphatic vessels. It was found that elevated TNF‐α secretion affects the mechanical properties of arteries by increasing the stiffness of vascular endothelial cells, 52 promoting intimal proliferation, 53 and stimulating collagen deposition by fibroblast 54 . In addition, TNF‐α can stimulate the expression of IL‐6 and production of adhesion molecules and other mediators that are responsible for endothelial leukocyte binding and the recruitment of leukocytes into the subendothelial tissues, which is important in atherogenesis 55 .…”

Section: Discussionmentioning

confidence: 99%

Abdominal aortic aneurysm and virus infection: A potential causative role for cytomegalovirus infection?

Jabłońska

Zagrapan

Paradowska

et al. 2021

Journal of Medical Virology

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An abdominal aortic aneurysm (AAA) is a multifactorial disease with a variety of genetic and environmental risk factors, but the exact mechanism of AAA formation and progression is still not well understood. The present study investigated the frequency of cytomegalovirus (CMV), Epstein‐Barr virus (EBV), and papillomavirus types 6 and 11 (HPV6 and HPV11), their impact on clinical manifestations of cardiovascular diseases, and their possible association with inflammation in patients with AAA and healthy volunteers. Genotyping of CMV UL75, EBV LMP‐1, and HPV6, and HPV11 E6 was performed by polymerase chain reaction (PCR), while the viral DNA loads were measured by quantitative real‐time PCR. Cytokine levels were determined by enzyme‐linked immunosorbent assays. The CMV UL75 was detected more frequently in the blood of patients with AAA than in the blood of healthy volunteers (32.7% vs. 6.3%, p < .0001). Neither EBV LMP‐1 nor HPV6 E6 was found in blood and aortic wall biopsies, while the HPV11 E6 was detected in 36.4% of AAA walls. The CMV infection in patients with AAA was associated with an increased risk of hypertension and coronary artery disease (OR, 9.057; 95% CI, 1.141–71.862; p = .037; and OR, 2.575; 95% CI, 1.002–6.615; p = .049, respectively). Additionally, CMV‐infected patients with AAA had higher tumor necrosis factor‐α levels compared with noninfected subjects (p = .017). Our findings suggest that CMV infection can stimulate local inflammation in the aorta but is not a direct cause of most abdominal aortic aneurysms.

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Section: Discussionmentioning

confidence: 99%

Abdominal aortic aneurysm and virus infection: A potential causative role for cytomegalovirus infection?

Jabłońska

Zagrapan

Paradowska

et al. 2021

Journal of Medical Virology

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“…8 TNF-α is a potent proinflammatory cytokine, and the plasma concentration of TNF-α is elevated in several pathologies, including cancer. 9 Wang et al reported that TNF-α induced epithelial− mesenchymal transition (EMT) in CRC. 10 In human endothelial cells, TNF-α stimulated ICAM-1 by activation of NADPH oxidase and Src.…”

Section: ■ Introductionmentioning

confidence: 99%

Andrographolide Antagonizes TNF-α-Induced IL-8 via Inhibition of NADPH Oxidase/ROS/NF-κB and Src/MAPKs/AP-1 Axis in Human Colorectal Cancer HCT116 Cells

Yuan

Wang

Liao

et al. 2018

J. Agric. Food Chem.

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Andrographis paniculata Nees is used as a functional food in Japan, Korea, India, and China. Andrographolide, a naturally occurring phytochemical identified in Andrographis paniculata, has been discovered to present anti-inflammatory and anticancer activities. Highly expressed interleukin (IL-8) has been detected in colorectal cancer and is implicated in angiogenesis. However, the effect and molecular mechanisms of IL-8 expression by andrographolide remain obscure in human colorectal cancer cells. The present study was aimed to investigate the effects of andrographolide on TNF-α-induced IL-8 expression and its underlying mechanisms. We found that andrographolide concentration-dependently inhibited TNF-α-induced IL-8 mRNA (2.23 ± 0.15 fold at 20 μM) and protein expression (4.78 ± 0.31 fold at 20 μM) and reduced the IL-8 transcriptional activity (2.59 ± 0.25 fold at 20 μM). TNF-α stimulated the membrane translocation of p47 to activate reactive oxygen species (ROS)-producing NADPH oxidase (NOX). Furthermore, TNF-α induced Src and MAPKs (Erk1/2, p38 MAPK) phosphorylation, as well as NF-κB and AP-1 binding activities. We found that NF-κB and AP-1 were the critical transcription factors for TNF-α-induced IL-8 expression. Specific inhibitors and mutagenesis studies indicated that Src, Erk1/2, and p38 MAPK are related to TNF-α-induced IL-8. NOX-derived ROS and Src/MAPKs (Erk1/2 and p38 MAPK) functioned as upstream activators of NF-κB and AP-1, respectively. Taken together, andrographolide antagonizes TNF-α-induced IL-8 via inhibition of NADPH oxidase/ROS/NF-κB and Src/MAPKs/AP-1 signaling pathways in HCT116 colorectal cancer cells and then suppresses angiogenesis in the tumor microenvironment.

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“…This remodeling results in dynamic alterations of the forces that cells transmit to their matrix and to each other, which are regulated to a large extent through the action of the actomyosin contractile apparatus (Komarova et al, 2017). Pro-inflammatory mediators and secreted cytokines can reinforce (Brandt et al, 2022) or alternatively release the tensile stresses between ECs, thus enhancing vascular permeability (Lee et al, 2011). A decrease in cell-ECM forces can also lead to attenuation in EC monolayer stresses as a result of the crosstalk between adherens junctions and focal adhesions (Komarova et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Borrelia burgdorferi modulates the physical forces and immunity signaling in endothelial cells

Aparicio-Yuste¹,

Muenkel²,

Axarlis³

et al. 2022

iScience

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Probing the mechanical properties of TNF-α stimulated endothelial cell with atomic force microscopy [Corrigendum]

Cited by 3 publications

References 8 publications

Abdominal aortic aneurysm and virus infection: A potential causative role for cytomegalovirus infection?

Abdominal aortic aneurysm and virus infection: A potential causative role for cytomegalovirus infection?

Andrographolide Antagonizes TNF-α-Induced IL-8 via Inhibition of NADPH Oxidase/ROS/NF-κB and Src/MAPKs/AP-1 Axis in Human Colorectal Cancer HCT116 Cells

Borrelia burgdorferi modulates the physical forces and immunity signaling in endothelial cells

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